Odontogenic ameloblast-associated (ODAM) is inactivated in toothless/enamelless placental mammals and toothed whales
Abstract Background The gene for odontogenic ameloblast-associated (ODAM) is a member of the secretory calcium-binding phosphoprotein gene family. ODAM is primarily expressed in dental tissues including the enamel organ and the junctional epithelium, and may also have pleiotropic functions that are...
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ftdoajarticles:oai:doaj.org/article:e0e85f562cb744a6afdeb5ca214bc1c5 2023-05-15T15:37:16+02:00 Odontogenic ameloblast-associated (ODAM) is inactivated in toothless/enamelless placental mammals and toothed whales Mark S. Springer Christopher A. Emerling John Gatesy Jason Randall Matthew A. Collin Nikolai Hecker Michael Hiller Frédéric Delsuc 2019-01-01T00:00:00Z https://doi.org/10.1186/s12862-019-1359-6 https://doaj.org/article/e0e85f562cb744a6afdeb5ca214bc1c5 EN eng BMC http://link.springer.com/article/10.1186/s12862-019-1359-6 https://doaj.org/toc/1471-2148 doi:10.1186/s12862-019-1359-6 1471-2148 https://doaj.org/article/e0e85f562cb744a6afdeb5ca214bc1c5 BMC Evolutionary Biology, Vol 19, Iss 1, Pp 1-19 (2019) Edentulism Enamel Junctional epithelium ODAM Pseudogene Evolution QH359-425 article 2019 ftdoajarticles https://doi.org/10.1186/s12862-019-1359-6 2022-12-31T04:37:04Z Abstract Background The gene for odontogenic ameloblast-associated (ODAM) is a member of the secretory calcium-binding phosphoprotein gene family. ODAM is primarily expressed in dental tissues including the enamel organ and the junctional epithelium, and may also have pleiotropic functions that are unrelated to teeth. Here, we leverage the power of natural selection to test competing hypotheses that ODAM is tooth-specific versus pleiotropic. Specifically, we compiled and screened complete protein-coding sequences, plus sequences for flanking intronic regions, for ODAM in 165 placental mammals to determine if this gene contains inactivating mutations in lineages that either lack teeth (baleen whales, pangolins, anteaters) or lack enamel on their teeth (aardvarks, sloths, armadillos), as would be expected if the only essential functions of ODAM are related to tooth development and the adhesion of the gingival junctional epithelium to the enamel tooth surface. Results We discovered inactivating mutations in all species of placental mammals that either lack teeth or lack enamel on their teeth. A surprising result is that ODAM is also inactivated in a few additional lineages including all toothed whales that were examined. We hypothesize that ODAM inactivation is related to the simplified outer enamel surface of toothed whales. An alternate hypothesis is that ODAM inactivation in toothed whales may be related to altered antimicrobial functions of the junctional epithelium in aquatic habitats. Selection analyses on ODAM sequences revealed that the composite dN/dS value for pseudogenic branches is close to 1.0 as expected for a neutrally evolving pseudogene. DN/dS values on transitional branches were used to estimate ODAM inactivation times. In the case of pangolins, ODAM was inactivated ~ 65 million years ago, which is older than the oldest pangolin fossil (Eomanis, 47 Ma) and suggests an even more ancient loss or simplification of teeth in this lineage. Conclusion Our results validate the hypothesis that the only ... Article in Journal/Newspaper baleen whales toothed whales Directory of Open Access Journals: DOAJ Articles BMC Evolutionary Biology 19 1 |
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Edentulism Enamel Junctional epithelium ODAM Pseudogene Evolution QH359-425 |
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Edentulism Enamel Junctional epithelium ODAM Pseudogene Evolution QH359-425 Mark S. Springer Christopher A. Emerling John Gatesy Jason Randall Matthew A. Collin Nikolai Hecker Michael Hiller Frédéric Delsuc Odontogenic ameloblast-associated (ODAM) is inactivated in toothless/enamelless placental mammals and toothed whales |
topic_facet |
Edentulism Enamel Junctional epithelium ODAM Pseudogene Evolution QH359-425 |
description |
Abstract Background The gene for odontogenic ameloblast-associated (ODAM) is a member of the secretory calcium-binding phosphoprotein gene family. ODAM is primarily expressed in dental tissues including the enamel organ and the junctional epithelium, and may also have pleiotropic functions that are unrelated to teeth. Here, we leverage the power of natural selection to test competing hypotheses that ODAM is tooth-specific versus pleiotropic. Specifically, we compiled and screened complete protein-coding sequences, plus sequences for flanking intronic regions, for ODAM in 165 placental mammals to determine if this gene contains inactivating mutations in lineages that either lack teeth (baleen whales, pangolins, anteaters) or lack enamel on their teeth (aardvarks, sloths, armadillos), as would be expected if the only essential functions of ODAM are related to tooth development and the adhesion of the gingival junctional epithelium to the enamel tooth surface. Results We discovered inactivating mutations in all species of placental mammals that either lack teeth or lack enamel on their teeth. A surprising result is that ODAM is also inactivated in a few additional lineages including all toothed whales that were examined. We hypothesize that ODAM inactivation is related to the simplified outer enamel surface of toothed whales. An alternate hypothesis is that ODAM inactivation in toothed whales may be related to altered antimicrobial functions of the junctional epithelium in aquatic habitats. Selection analyses on ODAM sequences revealed that the composite dN/dS value for pseudogenic branches is close to 1.0 as expected for a neutrally evolving pseudogene. DN/dS values on transitional branches were used to estimate ODAM inactivation times. In the case of pangolins, ODAM was inactivated ~ 65 million years ago, which is older than the oldest pangolin fossil (Eomanis, 47 Ma) and suggests an even more ancient loss or simplification of teeth in this lineage. Conclusion Our results validate the hypothesis that the only ... |
format |
Article in Journal/Newspaper |
author |
Mark S. Springer Christopher A. Emerling John Gatesy Jason Randall Matthew A. Collin Nikolai Hecker Michael Hiller Frédéric Delsuc |
author_facet |
Mark S. Springer Christopher A. Emerling John Gatesy Jason Randall Matthew A. Collin Nikolai Hecker Michael Hiller Frédéric Delsuc |
author_sort |
Mark S. Springer |
title |
Odontogenic ameloblast-associated (ODAM) is inactivated in toothless/enamelless placental mammals and toothed whales |
title_short |
Odontogenic ameloblast-associated (ODAM) is inactivated in toothless/enamelless placental mammals and toothed whales |
title_full |
Odontogenic ameloblast-associated (ODAM) is inactivated in toothless/enamelless placental mammals and toothed whales |
title_fullStr |
Odontogenic ameloblast-associated (ODAM) is inactivated in toothless/enamelless placental mammals and toothed whales |
title_full_unstemmed |
Odontogenic ameloblast-associated (ODAM) is inactivated in toothless/enamelless placental mammals and toothed whales |
title_sort |
odontogenic ameloblast-associated (odam) is inactivated in toothless/enamelless placental mammals and toothed whales |
publisher |
BMC |
publishDate |
2019 |
url |
https://doi.org/10.1186/s12862-019-1359-6 https://doaj.org/article/e0e85f562cb744a6afdeb5ca214bc1c5 |
genre |
baleen whales toothed whales |
genre_facet |
baleen whales toothed whales |
op_source |
BMC Evolutionary Biology, Vol 19, Iss 1, Pp 1-19 (2019) |
op_relation |
http://link.springer.com/article/10.1186/s12862-019-1359-6 https://doaj.org/toc/1471-2148 doi:10.1186/s12862-019-1359-6 1471-2148 https://doaj.org/article/e0e85f562cb744a6afdeb5ca214bc1c5 |
op_doi |
https://doi.org/10.1186/s12862-019-1359-6 |
container_title |
BMC Evolutionary Biology |
container_volume |
19 |
container_issue |
1 |
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1766367728128491520 |