Characterization of immunoglobulin G antibodies to Plasmodium falciparum sporozoite surface antigen MB 2 in malaria exposed individuals
Abstract Background MB2 protein is a sporozoite surface antigen on the human malaria parasite Plasmodium falciparum . MB2 was identified by screening a P. falciparum sporozoite cDNA expression library using immune sera from a protected donor immunized via the bites of P. falciparum -infected irradia...
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ftdoajarticles:oai:doaj.org/article:e0c8ec06408144398a6cf5f5f20c7202 2023-05-15T15:16:41+02:00 Characterization of immunoglobulin G antibodies to Plasmodium falciparum sporozoite surface antigen MB 2 in malaria exposed individuals John Chandy C de la Vega Patricia Sacci John B Nguyen Thanh V James Anthony A Kang Angray S 2009-10-01T00:00:00Z https://doi.org/10.1186/1475-2875-8-235 https://doaj.org/article/e0c8ec06408144398a6cf5f5f20c7202 EN eng BMC http://www.malariajournal.com/content/8/1/235 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-8-235 1475-2875 https://doaj.org/article/e0c8ec06408144398a6cf5f5f20c7202 Malaria Journal, Vol 8, Iss 1, p 235 (2009) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2009 ftdoajarticles https://doi.org/10.1186/1475-2875-8-235 2022-12-31T08:35:58Z Abstract Background MB2 protein is a sporozoite surface antigen on the human malaria parasite Plasmodium falciparum . MB2 was identified by screening a P. falciparum sporozoite cDNA expression library using immune sera from a protected donor immunized via the bites of P. falciparum -infected irradiated mosquitoes. It is not known whether natural exposure to P. falciparum also induces the anti-MB2 response and if this response differs from that in protected individuals immunized via the bites of P. falciparum infected irradiated mosquitoes. The anti-MB2 antibody response may be part of a robust protective response against the sporozoite. Methods Fragments of polypeptide regions of MB2 were constructed as recombinant fusions sandwiched between glutathione S-transferase and a hexa histidine tag for bacterial expression. The hexa histidine tag affinity purified proteins were used to immunize rabbits and the polyclonal sera evaluated in an in vitro inhibition of sporozoite invasion assay. The proteins were also used in immunoblots with sera from a limited number of donors immunized via the bites of P. falciparum infected irradiated mosquitoes and plasma and serum obtained from naturally exposed individuals in Kenya. Results Rabbit polyclonal antibodies targeting the non-repeat region of the basic domain of MB2 inhibited sporozoites entry into HepG2-A16 cells in vitro . Analysis of serum from five human volunteers that were immunized via the bites of P. falciparum infected irradiated mosquitoes that developed immunity and were completely protected against subsequent challenge with non-irradiated parasite also had detectable levels of antibody against MB2 basic domain. In contrast, in three volunteers not protected, anti-MB2 antibodies were below the level of detection. Sera from protected volunteers preferentially recognized a non-repeat region of the basic domain of MB2, whereas plasma from naturally-infected individuals also had antibodies that recognize regions of MB2 that contain a repeat motif in immunoblots. ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 8 1 |
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Directory of Open Access Journals: DOAJ Articles |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 John Chandy C de la Vega Patricia Sacci John B Nguyen Thanh V James Anthony A Kang Angray S Characterization of immunoglobulin G antibodies to Plasmodium falciparum sporozoite surface antigen MB 2 in malaria exposed individuals |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background MB2 protein is a sporozoite surface antigen on the human malaria parasite Plasmodium falciparum . MB2 was identified by screening a P. falciparum sporozoite cDNA expression library using immune sera from a protected donor immunized via the bites of P. falciparum -infected irradiated mosquitoes. It is not known whether natural exposure to P. falciparum also induces the anti-MB2 response and if this response differs from that in protected individuals immunized via the bites of P. falciparum infected irradiated mosquitoes. The anti-MB2 antibody response may be part of a robust protective response against the sporozoite. Methods Fragments of polypeptide regions of MB2 were constructed as recombinant fusions sandwiched between glutathione S-transferase and a hexa histidine tag for bacterial expression. The hexa histidine tag affinity purified proteins were used to immunize rabbits and the polyclonal sera evaluated in an in vitro inhibition of sporozoite invasion assay. The proteins were also used in immunoblots with sera from a limited number of donors immunized via the bites of P. falciparum infected irradiated mosquitoes and plasma and serum obtained from naturally exposed individuals in Kenya. Results Rabbit polyclonal antibodies targeting the non-repeat region of the basic domain of MB2 inhibited sporozoites entry into HepG2-A16 cells in vitro . Analysis of serum from five human volunteers that were immunized via the bites of P. falciparum infected irradiated mosquitoes that developed immunity and were completely protected against subsequent challenge with non-irradiated parasite also had detectable levels of antibody against MB2 basic domain. In contrast, in three volunteers not protected, anti-MB2 antibodies were below the level of detection. Sera from protected volunteers preferentially recognized a non-repeat region of the basic domain of MB2, whereas plasma from naturally-infected individuals also had antibodies that recognize regions of MB2 that contain a repeat motif in immunoblots. ... |
format |
Article in Journal/Newspaper |
author |
John Chandy C de la Vega Patricia Sacci John B Nguyen Thanh V James Anthony A Kang Angray S |
author_facet |
John Chandy C de la Vega Patricia Sacci John B Nguyen Thanh V James Anthony A Kang Angray S |
author_sort |
John Chandy C |
title |
Characterization of immunoglobulin G antibodies to Plasmodium falciparum sporozoite surface antigen MB 2 in malaria exposed individuals |
title_short |
Characterization of immunoglobulin G antibodies to Plasmodium falciparum sporozoite surface antigen MB 2 in malaria exposed individuals |
title_full |
Characterization of immunoglobulin G antibodies to Plasmodium falciparum sporozoite surface antigen MB 2 in malaria exposed individuals |
title_fullStr |
Characterization of immunoglobulin G antibodies to Plasmodium falciparum sporozoite surface antigen MB 2 in malaria exposed individuals |
title_full_unstemmed |
Characterization of immunoglobulin G antibodies to Plasmodium falciparum sporozoite surface antigen MB 2 in malaria exposed individuals |
title_sort |
characterization of immunoglobulin g antibodies to plasmodium falciparum sporozoite surface antigen mb 2 in malaria exposed individuals |
publisher |
BMC |
publishDate |
2009 |
url |
https://doi.org/10.1186/1475-2875-8-235 https://doaj.org/article/e0c8ec06408144398a6cf5f5f20c7202 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 8, Iss 1, p 235 (2009) |
op_relation |
http://www.malariajournal.com/content/8/1/235 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-8-235 1475-2875 https://doaj.org/article/e0c8ec06408144398a6cf5f5f20c7202 |
op_doi |
https://doi.org/10.1186/1475-2875-8-235 |
container_title |
Malaria Journal |
container_volume |
8 |
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1 |
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1766346987865636864 |