Genetic diversity and complexity of Plasmodium falciparum infections in the microenvironment among siblings of the same household in North-Central Nigeria
Abstract Background Plasmodium falciparum parasites are known to exhibit extensive genetic diversity in areas of high transmission intensity and infected individuals in such communities often harbour several complex mixtures of parasite clones with different genetic characteristics. However, in the...
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ftdoajarticles:oai:doaj.org/article:e09953a9475c47ae9de7fb8e369b0f1e 2023-05-15T15:18:12+02:00 Genetic diversity and complexity of Plasmodium falciparum infections in the microenvironment among siblings of the same household in North-Central Nigeria Segun Isaac Oyedeji Peter Usman Bassi Samuel Adeola Oyedeji Olusola Ojurongbe Henrietta Oluwatoyin Awobode 2020-09-01T00:00:00Z https://doi.org/10.1186/s12936-020-03415-1 https://doaj.org/article/e09953a9475c47ae9de7fb8e369b0f1e EN eng BMC http://link.springer.com/article/10.1186/s12936-020-03415-1 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03415-1 1475-2875 https://doaj.org/article/e09953a9475c47ae9de7fb8e369b0f1e Malaria Journal, Vol 19, Iss 1, Pp 1-10 (2020) Plasmodium falciparum Genetic diversity Malaria Multiplicity of infection MSP-2 Transmission intensity Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2020 ftdoajarticles https://doi.org/10.1186/s12936-020-03415-1 2022-12-31T12:21:12Z Abstract Background Plasmodium falciparum parasites are known to exhibit extensive genetic diversity in areas of high transmission intensity and infected individuals in such communities often harbour several complex mixtures of parasite clones with different genetic characteristics. However, in the micro-environment, the extent of genetic diversity of P. falciparum parasites remain largely unknown. In this study therefore, the complexity of P. falciparum infections in households was investigated among symptomatic siblings, living under the same roof in north-central Nigeria. Methods Children were enrolled into the study if they were at least two from a household and presented with symptoms of uncomplicated malaria. Clinical malaria was confirmed by light microscopy of Giemsa-stained thick and thin blood films. Genomic DNA was isolated from blood spots on filter paper. Molecular characterization of P. falciparum isolates was done by allele-specific nested PCR of the highly polymorphic merozoite surface protein-2 (msp-2) gene. Results Ninety-three children from 43 households were enrolled into this study. A total of 26 different msp-2 alleles were identified from 215 fragments (range: 180–480 bp). Majority of the isolates [65.6% (n = 61)] were polyclonal infections consisting of 2–6 clones and were significantly more common with the FC27 allelic family (p = 0.036). The multiplicity of infection (MOI) per household ranged from 1.0 to 4.5 while the overall MOI in the study population was 2.31. The pattern of distribution of msp-2 allele types among the households fell into two categories: households where both msp-2 allele types (FC27 and 3D7) were present; households where only one msp-2 allele type (FC27 or 3D7) was present. Majority of the households [88.4% (n = 38)], had both msp-2 allele types but they were disproportionately distributed among the children while in a few households [11.6% (n = 5)], all the children were infected with only one type of msp-2 allele. Conclusion These findings showed that P. ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 19 1 |
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topic |
Plasmodium falciparum Genetic diversity Malaria Multiplicity of infection MSP-2 Transmission intensity Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Plasmodium falciparum Genetic diversity Malaria Multiplicity of infection MSP-2 Transmission intensity Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Segun Isaac Oyedeji Peter Usman Bassi Samuel Adeola Oyedeji Olusola Ojurongbe Henrietta Oluwatoyin Awobode Genetic diversity and complexity of Plasmodium falciparum infections in the microenvironment among siblings of the same household in North-Central Nigeria |
topic_facet |
Plasmodium falciparum Genetic diversity Malaria Multiplicity of infection MSP-2 Transmission intensity Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Plasmodium falciparum parasites are known to exhibit extensive genetic diversity in areas of high transmission intensity and infected individuals in such communities often harbour several complex mixtures of parasite clones with different genetic characteristics. However, in the micro-environment, the extent of genetic diversity of P. falciparum parasites remain largely unknown. In this study therefore, the complexity of P. falciparum infections in households was investigated among symptomatic siblings, living under the same roof in north-central Nigeria. Methods Children were enrolled into the study if they were at least two from a household and presented with symptoms of uncomplicated malaria. Clinical malaria was confirmed by light microscopy of Giemsa-stained thick and thin blood films. Genomic DNA was isolated from blood spots on filter paper. Molecular characterization of P. falciparum isolates was done by allele-specific nested PCR of the highly polymorphic merozoite surface protein-2 (msp-2) gene. Results Ninety-three children from 43 households were enrolled into this study. A total of 26 different msp-2 alleles were identified from 215 fragments (range: 180–480 bp). Majority of the isolates [65.6% (n = 61)] were polyclonal infections consisting of 2–6 clones and were significantly more common with the FC27 allelic family (p = 0.036). The multiplicity of infection (MOI) per household ranged from 1.0 to 4.5 while the overall MOI in the study population was 2.31. The pattern of distribution of msp-2 allele types among the households fell into two categories: households where both msp-2 allele types (FC27 and 3D7) were present; households where only one msp-2 allele type (FC27 or 3D7) was present. Majority of the households [88.4% (n = 38)], had both msp-2 allele types but they were disproportionately distributed among the children while in a few households [11.6% (n = 5)], all the children were infected with only one type of msp-2 allele. Conclusion These findings showed that P. ... |
format |
Article in Journal/Newspaper |
author |
Segun Isaac Oyedeji Peter Usman Bassi Samuel Adeola Oyedeji Olusola Ojurongbe Henrietta Oluwatoyin Awobode |
author_facet |
Segun Isaac Oyedeji Peter Usman Bassi Samuel Adeola Oyedeji Olusola Ojurongbe Henrietta Oluwatoyin Awobode |
author_sort |
Segun Isaac Oyedeji |
title |
Genetic diversity and complexity of Plasmodium falciparum infections in the microenvironment among siblings of the same household in North-Central Nigeria |
title_short |
Genetic diversity and complexity of Plasmodium falciparum infections in the microenvironment among siblings of the same household in North-Central Nigeria |
title_full |
Genetic diversity and complexity of Plasmodium falciparum infections in the microenvironment among siblings of the same household in North-Central Nigeria |
title_fullStr |
Genetic diversity and complexity of Plasmodium falciparum infections in the microenvironment among siblings of the same household in North-Central Nigeria |
title_full_unstemmed |
Genetic diversity and complexity of Plasmodium falciparum infections in the microenvironment among siblings of the same household in North-Central Nigeria |
title_sort |
genetic diversity and complexity of plasmodium falciparum infections in the microenvironment among siblings of the same household in north-central nigeria |
publisher |
BMC |
publishDate |
2020 |
url |
https://doi.org/10.1186/s12936-020-03415-1 https://doaj.org/article/e09953a9475c47ae9de7fb8e369b0f1e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 19, Iss 1, Pp 1-10 (2020) |
op_relation |
http://link.springer.com/article/10.1186/s12936-020-03415-1 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03415-1 1475-2875 https://doaj.org/article/e09953a9475c47ae9de7fb8e369b0f1e |
op_doi |
https://doi.org/10.1186/s12936-020-03415-1 |
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Malaria Journal |
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19 |
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1 |
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1766348423888371712 |