Regulation and expression of sexual differentiation factors in embryonic and extragonadal tissues of Atlantic salmon
Abstract Background The products of cyp19 , dax , foxl2 , mis , sf1 and sox9 have each been associated with sex-determining processes among vertebrates. We provide evidence for expression of these regulators very early in salmonid development and in tissues outside of the hypothalamic-pituitary-adre...
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ftdoajarticles:oai:doaj.org/article:e039f4ec199748488b0c35316b8d4fd4 2023-05-15T15:32:58+02:00 Regulation and expression of sexual differentiation factors in embryonic and extragonadal tissues of Atlantic salmon Phillips Ruth B Rondeau Eric B Robb Adrienne So Stacy Reid Linda de Boer Johan G Yazawa Ryosuke Yasuike Motoshige von Schalburg Kristian R Davidson William S Koop Ben F 2011-01-01T00:00:00Z https://doi.org/10.1186/1471-2164-12-31 https://doaj.org/article/e039f4ec199748488b0c35316b8d4fd4 EN eng BMC http://www.biomedcentral.com/1471-2164/12/31 https://doaj.org/toc/1471-2164 doi:10.1186/1471-2164-12-31 1471-2164 https://doaj.org/article/e039f4ec199748488b0c35316b8d4fd4 BMC Genomics, Vol 12, Iss 1, p 31 (2011) Biotechnology TP248.13-248.65 Genetics QH426-470 article 2011 ftdoajarticles https://doi.org/10.1186/1471-2164-12-31 2022-12-31T12:55:01Z Abstract Background The products of cyp19 , dax , foxl2 , mis , sf1 and sox9 have each been associated with sex-determining processes among vertebrates. We provide evidence for expression of these regulators very early in salmonid development and in tissues outside of the hypothalamic-pituitary-adrenal/gonadal (HPAG) axis. Although the function of these factors in sexual differentiation have been defined, their roles in early development before sexual fate decisions and in tissues beyond the brain or gonad are essentially unknown. Results Bacterial artificial chromosomes containing salmon dax1 and dax2 , foxl2b and mis were isolated and the regulatory regions that control their expression were characterized. Transposon integrations are implicated in the shaping of the dax and foxl2 loci. Splice variants for cyp19b1 and mis in both embryonic and adult tissues were detected and characterized. We found that cyp19b1 transcripts are generated that contain 5'-untranslated regions of different lengths due to cryptic splicing of the 3'-end of intron 1. We also demonstrate that salmon mis transcripts can encode prodomain products that present different C-termini and terminate before translation of the MIS hormone. Regulatory differences in the expression of two distinct aromatases cyp19a and cyp19b1 are exerted, despite transcription of their transactivators (ie; dax1 , foxl2 , sf1 ) occurring much earlier during embryonic development. Conclusions We report the embryonic and extragonadal expression of dax , foxl2 , mis and other differentiation factors that indicate that they have functions that are more general and not restricted to steroidogenesis and gonadogenesis. Spliced cyp19b1 and mis transcripts are generated that may provide regulatory controls for tissue- or development-specific activities. Selection of cyp19b1 transcripts may be regulated by DAX-1, FOXL2 and SF-1 complexes that bind motifs in intron 1, or by signals within exon 2 that recruit splicing factors, or both. The potential translation of proteins ... Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles BMC Genomics 12 1 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Biotechnology TP248.13-248.65 Genetics QH426-470 |
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Biotechnology TP248.13-248.65 Genetics QH426-470 Phillips Ruth B Rondeau Eric B Robb Adrienne So Stacy Reid Linda de Boer Johan G Yazawa Ryosuke Yasuike Motoshige von Schalburg Kristian R Davidson William S Koop Ben F Regulation and expression of sexual differentiation factors in embryonic and extragonadal tissues of Atlantic salmon |
topic_facet |
Biotechnology TP248.13-248.65 Genetics QH426-470 |
description |
Abstract Background The products of cyp19 , dax , foxl2 , mis , sf1 and sox9 have each been associated with sex-determining processes among vertebrates. We provide evidence for expression of these regulators very early in salmonid development and in tissues outside of the hypothalamic-pituitary-adrenal/gonadal (HPAG) axis. Although the function of these factors in sexual differentiation have been defined, their roles in early development before sexual fate decisions and in tissues beyond the brain or gonad are essentially unknown. Results Bacterial artificial chromosomes containing salmon dax1 and dax2 , foxl2b and mis were isolated and the regulatory regions that control their expression were characterized. Transposon integrations are implicated in the shaping of the dax and foxl2 loci. Splice variants for cyp19b1 and mis in both embryonic and adult tissues were detected and characterized. We found that cyp19b1 transcripts are generated that contain 5'-untranslated regions of different lengths due to cryptic splicing of the 3'-end of intron 1. We also demonstrate that salmon mis transcripts can encode prodomain products that present different C-termini and terminate before translation of the MIS hormone. Regulatory differences in the expression of two distinct aromatases cyp19a and cyp19b1 are exerted, despite transcription of their transactivators (ie; dax1 , foxl2 , sf1 ) occurring much earlier during embryonic development. Conclusions We report the embryonic and extragonadal expression of dax , foxl2 , mis and other differentiation factors that indicate that they have functions that are more general and not restricted to steroidogenesis and gonadogenesis. Spliced cyp19b1 and mis transcripts are generated that may provide regulatory controls for tissue- or development-specific activities. Selection of cyp19b1 transcripts may be regulated by DAX-1, FOXL2 and SF-1 complexes that bind motifs in intron 1, or by signals within exon 2 that recruit splicing factors, or both. The potential translation of proteins ... |
format |
Article in Journal/Newspaper |
author |
Phillips Ruth B Rondeau Eric B Robb Adrienne So Stacy Reid Linda de Boer Johan G Yazawa Ryosuke Yasuike Motoshige von Schalburg Kristian R Davidson William S Koop Ben F |
author_facet |
Phillips Ruth B Rondeau Eric B Robb Adrienne So Stacy Reid Linda de Boer Johan G Yazawa Ryosuke Yasuike Motoshige von Schalburg Kristian R Davidson William S Koop Ben F |
author_sort |
Phillips Ruth B |
title |
Regulation and expression of sexual differentiation factors in embryonic and extragonadal tissues of Atlantic salmon |
title_short |
Regulation and expression of sexual differentiation factors in embryonic and extragonadal tissues of Atlantic salmon |
title_full |
Regulation and expression of sexual differentiation factors in embryonic and extragonadal tissues of Atlantic salmon |
title_fullStr |
Regulation and expression of sexual differentiation factors in embryonic and extragonadal tissues of Atlantic salmon |
title_full_unstemmed |
Regulation and expression of sexual differentiation factors in embryonic and extragonadal tissues of Atlantic salmon |
title_sort |
regulation and expression of sexual differentiation factors in embryonic and extragonadal tissues of atlantic salmon |
publisher |
BMC |
publishDate |
2011 |
url |
https://doi.org/10.1186/1471-2164-12-31 https://doaj.org/article/e039f4ec199748488b0c35316b8d4fd4 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
BMC Genomics, Vol 12, Iss 1, p 31 (2011) |
op_relation |
http://www.biomedcentral.com/1471-2164/12/31 https://doaj.org/toc/1471-2164 doi:10.1186/1471-2164-12-31 1471-2164 https://doaj.org/article/e039f4ec199748488b0c35316b8d4fd4 |
op_doi |
https://doi.org/10.1186/1471-2164-12-31 |
container_title |
BMC Genomics |
container_volume |
12 |
container_issue |
1 |
_version_ |
1766363450451165184 |