Helminth species dependent effects on Th1 and Th17 cytokines in active tuberculosis patients and healthy community controls.

Despite that the impact of different helminth species is not well explored, the current dogma states that helminths affect the Th1/Th2 balance which in turn affects the risk of tuberculosis (TB) reactivation and severity of disease. We investigated the influence of helminth species on cytokine profi...

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Published in:PLOS Neglected Tropical Diseases
Main Authors: Gezahegn Bewket, Amare Kiflie, Fitsumbrhan Tajebe, Ebba Abate, Thomas Schön, Robert Blomgran
Format: Article in Journal/Newspaper
Language:English
Published: Public Library of Science (PLoS) 2022
Subjects:
Online Access:https://doi.org/10.1371/journal.pntd.0010721
https://doaj.org/article/e0223949bd6b4f308d20571cdaefcfd2
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spelling ftdoajarticles:oai:doaj.org/article:e0223949bd6b4f308d20571cdaefcfd2 2023-05-15T15:18:26+02:00 Helminth species dependent effects on Th1 and Th17 cytokines in active tuberculosis patients and healthy community controls. Gezahegn Bewket Amare Kiflie Fitsumbrhan Tajebe Ebba Abate Thomas Schön Robert Blomgran 2022-08-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010721 https://doaj.org/article/e0223949bd6b4f308d20571cdaefcfd2 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010721 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010721 https://doaj.org/article/e0223949bd6b4f308d20571cdaefcfd2 PLoS Neglected Tropical Diseases, Vol 16, Iss 8, p e0010721 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010721 2022-12-30T21:59:03Z Despite that the impact of different helminth species is not well explored, the current dogma states that helminths affect the Th1/Th2 balance which in turn affects the risk of tuberculosis (TB) reactivation and severity of disease. We investigated the influence of helminth species on cytokine profiles including IL-17A in TB patients and healthy community controls (CCs). In total, 104 newly diagnosed pulmonary TB patients and 70 HIV negative and QuantiFERON negative CCs in Gondar, Ethiopia were included following helminth screening by stool microscopy. Plasma samples and ex vivo stimulation of peripheral blood mononuclear cells (PBMCs) with purified protein derivative (PPD) and Staphylococcus enterotoxin B (SEB) was used to determine cytokine profiles by cytometric bead array. In CCs, Ascaris lumbricoides or Schistosoma mansoni infections were associated with an impaired Th1-type response (IFN-gamma, IL-6 and TNF-alpha) in PBMCs mainly with SEB stimulations, whereas in TB patients only hookworm infection showed a similar pattern. Among CCs, the IL-17A response in PBMCs stimulated with SEB was higher only for S. mansoni, whereas in TB patients, the elevated systemic IL-17A plasma level was significantly suppressed in hookworm infected TB patients compared to patients without helminth coinfection. Following treatment of TB and helminth infection there was a general decrease in ex vivio IL-10 and TNF-alpha production in unstimulated, PPD or SEB stimulated PBMCs that was the most pronounced and significant in TB patients infected with S. mansoni, whereas the follow-up levels of IFN-gamma and IL-17A was significantly increased only in TB patients without helminth coinfection from PBMCs stimulated mainly with SEB. In summary, in addition to confirming helminth specific effects on the Th1/Th2 response before and after TB treatment, our novel finding is that IL-17A was impaired in helminth infected TB patients especially for hookworm, indicating a helminth species-specific immunoregulatory effect on IL-17A which needs ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 16 8 e0010721
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic Arctic medicine. Tropical medicine
RC955-962
Public aspects of medicine
RA1-1270
spellingShingle Arctic medicine. Tropical medicine
RC955-962
Public aspects of medicine
RA1-1270
Gezahegn Bewket
Amare Kiflie
Fitsumbrhan Tajebe
Ebba Abate
Thomas Schön
Robert Blomgran
Helminth species dependent effects on Th1 and Th17 cytokines in active tuberculosis patients and healthy community controls.
topic_facet Arctic medicine. Tropical medicine
RC955-962
Public aspects of medicine
RA1-1270
description Despite that the impact of different helminth species is not well explored, the current dogma states that helminths affect the Th1/Th2 balance which in turn affects the risk of tuberculosis (TB) reactivation and severity of disease. We investigated the influence of helminth species on cytokine profiles including IL-17A in TB patients and healthy community controls (CCs). In total, 104 newly diagnosed pulmonary TB patients and 70 HIV negative and QuantiFERON negative CCs in Gondar, Ethiopia were included following helminth screening by stool microscopy. Plasma samples and ex vivo stimulation of peripheral blood mononuclear cells (PBMCs) with purified protein derivative (PPD) and Staphylococcus enterotoxin B (SEB) was used to determine cytokine profiles by cytometric bead array. In CCs, Ascaris lumbricoides or Schistosoma mansoni infections were associated with an impaired Th1-type response (IFN-gamma, IL-6 and TNF-alpha) in PBMCs mainly with SEB stimulations, whereas in TB patients only hookworm infection showed a similar pattern. Among CCs, the IL-17A response in PBMCs stimulated with SEB was higher only for S. mansoni, whereas in TB patients, the elevated systemic IL-17A plasma level was significantly suppressed in hookworm infected TB patients compared to patients without helminth coinfection. Following treatment of TB and helminth infection there was a general decrease in ex vivio IL-10 and TNF-alpha production in unstimulated, PPD or SEB stimulated PBMCs that was the most pronounced and significant in TB patients infected with S. mansoni, whereas the follow-up levels of IFN-gamma and IL-17A was significantly increased only in TB patients without helminth coinfection from PBMCs stimulated mainly with SEB. In summary, in addition to confirming helminth specific effects on the Th1/Th2 response before and after TB treatment, our novel finding is that IL-17A was impaired in helminth infected TB patients especially for hookworm, indicating a helminth species-specific immunoregulatory effect on IL-17A which needs ...
format Article in Journal/Newspaper
author Gezahegn Bewket
Amare Kiflie
Fitsumbrhan Tajebe
Ebba Abate
Thomas Schön
Robert Blomgran
author_facet Gezahegn Bewket
Amare Kiflie
Fitsumbrhan Tajebe
Ebba Abate
Thomas Schön
Robert Blomgran
author_sort Gezahegn Bewket
title Helminth species dependent effects on Th1 and Th17 cytokines in active tuberculosis patients and healthy community controls.
title_short Helminth species dependent effects on Th1 and Th17 cytokines in active tuberculosis patients and healthy community controls.
title_full Helminth species dependent effects on Th1 and Th17 cytokines in active tuberculosis patients and healthy community controls.
title_fullStr Helminth species dependent effects on Th1 and Th17 cytokines in active tuberculosis patients and healthy community controls.
title_full_unstemmed Helminth species dependent effects on Th1 and Th17 cytokines in active tuberculosis patients and healthy community controls.
title_sort helminth species dependent effects on th1 and th17 cytokines in active tuberculosis patients and healthy community controls.
publisher Public Library of Science (PLoS)
publishDate 2022
url https://doi.org/10.1371/journal.pntd.0010721
https://doaj.org/article/e0223949bd6b4f308d20571cdaefcfd2
geographic Arctic
geographic_facet Arctic
genre Arctic
genre_facet Arctic
op_source PLoS Neglected Tropical Diseases, Vol 16, Iss 8, p e0010721 (2022)
op_relation https://doi.org/10.1371/journal.pntd.0010721
https://doaj.org/toc/1935-2727
https://doaj.org/toc/1935-2735
1935-2727
1935-2735
doi:10.1371/journal.pntd.0010721
https://doaj.org/article/e0223949bd6b4f308d20571cdaefcfd2
op_doi https://doi.org/10.1371/journal.pntd.0010721
container_title PLOS Neglected Tropical Diseases
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