The regulation of autophagy differentially affects Trypanosoma cruzi metacyclogenesis.
Autophagy is a cellular process required for the removal of aged organelles and cytosolic components through lysosomal degradation. All types of eukaryotic cells from yeasts to mammalian cells have the machinery to activate autophagy as a result of many physiological and pathological situations. The...
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ftdoajarticles:oai:doaj.org/article:e01d56cbfc5a46a4a863ecf255a2a42b 2023-05-15T15:10:27+02:00 The regulation of autophagy differentially affects Trypanosoma cruzi metacyclogenesis. María Cristina Vanrell Antonella Denisse Losinno Juan Agustín Cueto Darío Balcazar Laura Virginia Fraccaroli Carolina Carrillo Patricia Silvia Romano 2017-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006049 https://doaj.org/article/e01d56cbfc5a46a4a863ecf255a2a42b EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5683653?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006049 https://doaj.org/article/e01d56cbfc5a46a4a863ecf255a2a42b PLoS Neglected Tropical Diseases, Vol 11, Iss 11, p e0006049 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006049 2022-12-31T00:59:13Z Autophagy is a cellular process required for the removal of aged organelles and cytosolic components through lysosomal degradation. All types of eukaryotic cells from yeasts to mammalian cells have the machinery to activate autophagy as a result of many physiological and pathological situations. The most frequent stimulus of autophagy is starvation and the result, in this case, is the fast generation of utilizable food (e.g. amino acids and basic nutrients) to maintain the vital biological processes. In some organisms, starvation also triggers other associated processes such as differentiation. The protozoan parasite Trypanosoma cruzi undergoes a series of differentiation processes throughout its complex life cycle. Although not all autophagic genes have been identified in the T. cruzi genome, previous works have demonstrated the presence of essential autophagic-related proteins. Under starvation conditions, TcAtg8, which is the parasite homolog of Atg8/LC3 in other organisms, is located in autophagosome-like vesicles. In this work, we have characterized the autophagic pathway during T. cruzi differentiation from the epimastigote to metacyclic trypomastigote form, a process called metacyclogenesis. We demonstrated that autophagy is stimulated during metacyclogenesis and that the induction of autophagy promotes this process. Moreover, with exception of bafilomycin, other classical autophagy modulators have similar effects on T. cruzi autophagy. We also showed that spermidine and related polyamines can positively regulate parasite autophagy and differentiation. We concluded that both polyamine metabolism and autophagy are key processes during T. cruzi metacyclogenesis that could be exploited as drug targets to avoid the parasite cycle progression. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 11 e0006049 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 María Cristina Vanrell Antonella Denisse Losinno Juan Agustín Cueto Darío Balcazar Laura Virginia Fraccaroli Carolina Carrillo Patricia Silvia Romano The regulation of autophagy differentially affects Trypanosoma cruzi metacyclogenesis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Autophagy is a cellular process required for the removal of aged organelles and cytosolic components through lysosomal degradation. All types of eukaryotic cells from yeasts to mammalian cells have the machinery to activate autophagy as a result of many physiological and pathological situations. The most frequent stimulus of autophagy is starvation and the result, in this case, is the fast generation of utilizable food (e.g. amino acids and basic nutrients) to maintain the vital biological processes. In some organisms, starvation also triggers other associated processes such as differentiation. The protozoan parasite Trypanosoma cruzi undergoes a series of differentiation processes throughout its complex life cycle. Although not all autophagic genes have been identified in the T. cruzi genome, previous works have demonstrated the presence of essential autophagic-related proteins. Under starvation conditions, TcAtg8, which is the parasite homolog of Atg8/LC3 in other organisms, is located in autophagosome-like vesicles. In this work, we have characterized the autophagic pathway during T. cruzi differentiation from the epimastigote to metacyclic trypomastigote form, a process called metacyclogenesis. We demonstrated that autophagy is stimulated during metacyclogenesis and that the induction of autophagy promotes this process. Moreover, with exception of bafilomycin, other classical autophagy modulators have similar effects on T. cruzi autophagy. We also showed that spermidine and related polyamines can positively regulate parasite autophagy and differentiation. We concluded that both polyamine metabolism and autophagy are key processes during T. cruzi metacyclogenesis that could be exploited as drug targets to avoid the parasite cycle progression. |
format |
Article in Journal/Newspaper |
author |
María Cristina Vanrell Antonella Denisse Losinno Juan Agustín Cueto Darío Balcazar Laura Virginia Fraccaroli Carolina Carrillo Patricia Silvia Romano |
author_facet |
María Cristina Vanrell Antonella Denisse Losinno Juan Agustín Cueto Darío Balcazar Laura Virginia Fraccaroli Carolina Carrillo Patricia Silvia Romano |
author_sort |
María Cristina Vanrell |
title |
The regulation of autophagy differentially affects Trypanosoma cruzi metacyclogenesis. |
title_short |
The regulation of autophagy differentially affects Trypanosoma cruzi metacyclogenesis. |
title_full |
The regulation of autophagy differentially affects Trypanosoma cruzi metacyclogenesis. |
title_fullStr |
The regulation of autophagy differentially affects Trypanosoma cruzi metacyclogenesis. |
title_full_unstemmed |
The regulation of autophagy differentially affects Trypanosoma cruzi metacyclogenesis. |
title_sort |
regulation of autophagy differentially affects trypanosoma cruzi metacyclogenesis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0006049 https://doaj.org/article/e01d56cbfc5a46a4a863ecf255a2a42b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 11, p e0006049 (2017) |
op_relation |
http://europepmc.org/articles/PMC5683653?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006049 https://doaj.org/article/e01d56cbfc5a46a4a863ecf255a2a42b |
op_doi |
https://doi.org/10.1371/journal.pntd.0006049 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
11 |
container_start_page |
e0006049 |
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1766341477912281088 |