Diverse Genotypes of Yersinia pestis Caused Plague in Madagascar in 2007.
BACKGROUND:Yersinia pestis is the causative agent of human plague and is endemic in various African, Asian and American countries. In Madagascar, the disease represents a significant public health problem with hundreds of human cases a year. Unfortunately, poor infrastructure makes outbreak investig...
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ftdoajarticles:oai:doaj.org/article:dfc1bd39390b42a88d20ca024c9f9044 2023-05-15T15:06:33+02:00 Diverse Genotypes of Yersinia pestis Caused Plague in Madagascar in 2007. Julia M Riehm Michaela Projahn Amy J Vogler Minoaerisoa Rajerison Genevieve Andersen Carina M Hall Thomas Zimmermann Rahelinirina Soanandrasana Voahangy Andrianaivoarimanana Reinhard K Straubinger Roxanne Nottingham Paul Keim David M Wagner Holger C Scholz 2015-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003844 https://doaj.org/article/dfc1bd39390b42a88d20ca024c9f9044 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4466568?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003844 https://doaj.org/article/dfc1bd39390b42a88d20ca024c9f9044 PLoS Neglected Tropical Diseases, Vol 9, Iss 6, p e0003844 (2015) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2015 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003844 2022-12-31T01:15:50Z BACKGROUND:Yersinia pestis is the causative agent of human plague and is endemic in various African, Asian and American countries. In Madagascar, the disease represents a significant public health problem with hundreds of human cases a year. Unfortunately, poor infrastructure makes outbreak investigations challenging. METHODOLOGY/PRINCIPAL FINDINGS:DNA was extracted directly from 93 clinical samples from patients with a clinical diagnosis of plague in Madagascar in 2007. The extracted DNAs were then genotyped using three molecular genotyping methods, including, single nucleotide polymorphism (SNP) typing, multi-locus variable-number tandem repeat analysis (MLVA), and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) analysis. These methods provided increasing resolution, respectively. The results of these analyses revealed that, in 2007, ten molecular groups, two newly described here and eight previously identified, were responsible for causing human plague in geographically distinct areas of Madagascar. CONCLUSIONS/SIGNIFICANCE:Plague in Madagascar is caused by numerous distinct types of Y. pestis. Genotyping method choice should be based upon the discriminatory power needed, expense, and available data for any desired comparisons. We conclude that genotyping should be a standard tool used in epidemiological investigations of plague outbreaks. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 9 6 e0003844 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Julia M Riehm Michaela Projahn Amy J Vogler Minoaerisoa Rajerison Genevieve Andersen Carina M Hall Thomas Zimmermann Rahelinirina Soanandrasana Voahangy Andrianaivoarimanana Reinhard K Straubinger Roxanne Nottingham Paul Keim David M Wagner Holger C Scholz Diverse Genotypes of Yersinia pestis Caused Plague in Madagascar in 2007. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Yersinia pestis is the causative agent of human plague and is endemic in various African, Asian and American countries. In Madagascar, the disease represents a significant public health problem with hundreds of human cases a year. Unfortunately, poor infrastructure makes outbreak investigations challenging. METHODOLOGY/PRINCIPAL FINDINGS:DNA was extracted directly from 93 clinical samples from patients with a clinical diagnosis of plague in Madagascar in 2007. The extracted DNAs were then genotyped using three molecular genotyping methods, including, single nucleotide polymorphism (SNP) typing, multi-locus variable-number tandem repeat analysis (MLVA), and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) analysis. These methods provided increasing resolution, respectively. The results of these analyses revealed that, in 2007, ten molecular groups, two newly described here and eight previously identified, were responsible for causing human plague in geographically distinct areas of Madagascar. CONCLUSIONS/SIGNIFICANCE:Plague in Madagascar is caused by numerous distinct types of Y. pestis. Genotyping method choice should be based upon the discriminatory power needed, expense, and available data for any desired comparisons. We conclude that genotyping should be a standard tool used in epidemiological investigations of plague outbreaks. |
format |
Article in Journal/Newspaper |
author |
Julia M Riehm Michaela Projahn Amy J Vogler Minoaerisoa Rajerison Genevieve Andersen Carina M Hall Thomas Zimmermann Rahelinirina Soanandrasana Voahangy Andrianaivoarimanana Reinhard K Straubinger Roxanne Nottingham Paul Keim David M Wagner Holger C Scholz |
author_facet |
Julia M Riehm Michaela Projahn Amy J Vogler Minoaerisoa Rajerison Genevieve Andersen Carina M Hall Thomas Zimmermann Rahelinirina Soanandrasana Voahangy Andrianaivoarimanana Reinhard K Straubinger Roxanne Nottingham Paul Keim David M Wagner Holger C Scholz |
author_sort |
Julia M Riehm |
title |
Diverse Genotypes of Yersinia pestis Caused Plague in Madagascar in 2007. |
title_short |
Diverse Genotypes of Yersinia pestis Caused Plague in Madagascar in 2007. |
title_full |
Diverse Genotypes of Yersinia pestis Caused Plague in Madagascar in 2007. |
title_fullStr |
Diverse Genotypes of Yersinia pestis Caused Plague in Madagascar in 2007. |
title_full_unstemmed |
Diverse Genotypes of Yersinia pestis Caused Plague in Madagascar in 2007. |
title_sort |
diverse genotypes of yersinia pestis caused plague in madagascar in 2007. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pntd.0003844 https://doaj.org/article/dfc1bd39390b42a88d20ca024c9f9044 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 9, Iss 6, p e0003844 (2015) |
op_relation |
http://europepmc.org/articles/PMC4466568?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003844 https://doaj.org/article/dfc1bd39390b42a88d20ca024c9f9044 |
op_doi |
https://doi.org/10.1371/journal.pntd.0003844 |
container_title |
PLOS Neglected Tropical Diseases |
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9 |
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6 |
container_start_page |
e0003844 |
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