Bothrops lanceolatus snake venom impairs mitochondrial respiration and induces DNA release in human heart preparation.
Introduction Envenomations by Bothrops snakebites can induce overwhelming systemic inflammation ultimately leading to multiple organ system failure and death. Release of damage-associated molecular pattern molecules (DAMPs), in particular of mitochondrial origin, has been implicated in the pathophys...
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ftdoajarticles:oai:doaj.org/article:dfb1b09b15764c1cb5e96365fe4a5250 2023-05-15T15:07:23+02:00 Bothrops lanceolatus snake venom impairs mitochondrial respiration and induces DNA release in human heart preparation. Mariola Cano-Sanchez Kais Ben-Hassen Olivier Pierre Louis Fabienne Dantin Papa Gueye Francois Roques Hossein Mehdaoui Dabor Resiere Remi Neviere 2022-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010523 https://doaj.org/article/dfb1b09b15764c1cb5e96365fe4a5250 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010523 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010523 https://doaj.org/article/dfb1b09b15764c1cb5e96365fe4a5250 PLoS Neglected Tropical Diseases, Vol 16, Iss 6, p e0010523 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010523 2022-12-31T02:23:58Z Introduction Envenomations by Bothrops snakebites can induce overwhelming systemic inflammation ultimately leading to multiple organ system failure and death. Release of damage-associated molecular pattern molecules (DAMPs), in particular of mitochondrial origin, has been implicated in the pathophysiology of the deregulated innate immune response. Objective To test whether whole Bothrops lanceolatus venom would induce mitochondrial dysfunction and DAMPs release in human heart preparations. Methods Human atrial trabeculae were obtained during cannulation for cardiopulmonary bypass from patients who were undergoing routine coronary artery bypass surgery. Cardiac fibers were incubated with vehicle and whole Bothrops lanceolatus venom for 24hr before high-resolution respirometry, mitochondrial membrane permeability evaluation and quantification of mitochondrial DNA. Results Compared with vehicle, incubation of human cardiac muscle with whole Bothrops lanceolatus venom for 24hr impaired respiratory control ratio and mitochondrial membrane permeability. Levels of mitochondrial DNA increased in the medium of cardiac cell preparation incubated with venom of Bothrops lanceolatus. Conclusion Our study suggests that whole venom of Bothrops lanceolatus impairs mitochondrial oxidative phosphorylation capacity and increases mitochondrial membrane permeability. Cardiac mitochondrial dysfunction associated with mitochondrial DAMPs release may alter myocardium function and engage the innate immune response, which may both participate to the cardiotoxicity occurring in patients with severe envenomation. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 16 6 e0010523 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Mariola Cano-Sanchez Kais Ben-Hassen Olivier Pierre Louis Fabienne Dantin Papa Gueye Francois Roques Hossein Mehdaoui Dabor Resiere Remi Neviere Bothrops lanceolatus snake venom impairs mitochondrial respiration and induces DNA release in human heart preparation. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Introduction Envenomations by Bothrops snakebites can induce overwhelming systemic inflammation ultimately leading to multiple organ system failure and death. Release of damage-associated molecular pattern molecules (DAMPs), in particular of mitochondrial origin, has been implicated in the pathophysiology of the deregulated innate immune response. Objective To test whether whole Bothrops lanceolatus venom would induce mitochondrial dysfunction and DAMPs release in human heart preparations. Methods Human atrial trabeculae were obtained during cannulation for cardiopulmonary bypass from patients who were undergoing routine coronary artery bypass surgery. Cardiac fibers were incubated with vehicle and whole Bothrops lanceolatus venom for 24hr before high-resolution respirometry, mitochondrial membrane permeability evaluation and quantification of mitochondrial DNA. Results Compared with vehicle, incubation of human cardiac muscle with whole Bothrops lanceolatus venom for 24hr impaired respiratory control ratio and mitochondrial membrane permeability. Levels of mitochondrial DNA increased in the medium of cardiac cell preparation incubated with venom of Bothrops lanceolatus. Conclusion Our study suggests that whole venom of Bothrops lanceolatus impairs mitochondrial oxidative phosphorylation capacity and increases mitochondrial membrane permeability. Cardiac mitochondrial dysfunction associated with mitochondrial DAMPs release may alter myocardium function and engage the innate immune response, which may both participate to the cardiotoxicity occurring in patients with severe envenomation. |
format |
Article in Journal/Newspaper |
author |
Mariola Cano-Sanchez Kais Ben-Hassen Olivier Pierre Louis Fabienne Dantin Papa Gueye Francois Roques Hossein Mehdaoui Dabor Resiere Remi Neviere |
author_facet |
Mariola Cano-Sanchez Kais Ben-Hassen Olivier Pierre Louis Fabienne Dantin Papa Gueye Francois Roques Hossein Mehdaoui Dabor Resiere Remi Neviere |
author_sort |
Mariola Cano-Sanchez |
title |
Bothrops lanceolatus snake venom impairs mitochondrial respiration and induces DNA release in human heart preparation. |
title_short |
Bothrops lanceolatus snake venom impairs mitochondrial respiration and induces DNA release in human heart preparation. |
title_full |
Bothrops lanceolatus snake venom impairs mitochondrial respiration and induces DNA release in human heart preparation. |
title_fullStr |
Bothrops lanceolatus snake venom impairs mitochondrial respiration and induces DNA release in human heart preparation. |
title_full_unstemmed |
Bothrops lanceolatus snake venom impairs mitochondrial respiration and induces DNA release in human heart preparation. |
title_sort |
bothrops lanceolatus snake venom impairs mitochondrial respiration and induces dna release in human heart preparation. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0010523 https://doaj.org/article/dfb1b09b15764c1cb5e96365fe4a5250 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 16, Iss 6, p e0010523 (2022) |
op_relation |
https://doi.org/10.1371/journal.pntd.0010523 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010523 https://doaj.org/article/dfb1b09b15764c1cb5e96365fe4a5250 |
op_doi |
https://doi.org/10.1371/journal.pntd.0010523 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
16 |
container_issue |
6 |
container_start_page |
e0010523 |
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1766338903471554560 |