Assessment of biomedical and pharmacological activities of sea anemones Stichodactyla mertensii and Stichodactyla gigantea from Gulf of Mannar Biosphere Reserve, southeast coast of India
Cnidarians comprise an old and diverse animal phylum, and possess a wide variety of biologically active substances. Sea anemones contain a diversity of interesting biologically active compounds including some potent toxins. In the present work, the sea anemones Stichodactyla mertensii and Stichodact...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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Online Access: | https://doi.org/10.1590/S1678-91992012000100007 https://doaj.org/article/df6d0b2ca4fe4c9084cce3cfe28d30f9 |
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ftdoajarticles:oai:doaj.org/article:df6d0b2ca4fe4c9084cce3cfe28d30f9 2023-05-15T15:13:56+02:00 Assessment of biomedical and pharmacological activities of sea anemones Stichodactyla mertensii and Stichodactyla gigantea from Gulf of Mannar Biosphere Reserve, southeast coast of India S Thangaraj S Bragadeeswaran 2012-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992012000100007 https://doaj.org/article/df6d0b2ca4fe4c9084cce3cfe28d30f9 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100007 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992012000100007 1678-9199 https://doaj.org/article/df6d0b2ca4fe4c9084cce3cfe28d30f9 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 18, Iss 1, Pp 53-61 (2012) aqueous extract neurotoxicity mouse bioassay analgesic activity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2012 ftdoajarticles https://doi.org/10.1590/S1678-91992012000100007 2022-12-31T11:33:11Z Cnidarians comprise an old and diverse animal phylum, and possess a wide variety of biologically active substances. Sea anemones contain a diversity of interesting biologically active compounds including some potent toxins. In the present work, the sea anemones Stichodactyla mertensii and Stichodactyla gigantea, collected from the Mandapam coast, are characterized biomedically and pharmacologically. The crude protein was obtained by using methanol and aqueous extracts. The respective protein contents of S. mertensii and S. gigantea were found to be 2.10 µg/mL and 1.87 µg/mL. The methanol and aqueous extracts of S. mertensii and S. gigantea yielded six and nine bands by SDS-PAGE on 12% gel. In the hemolytic assay, both extracts exhibited hemolytic effect on chicken, goat, cow and human erythrocytes ('A', 'B' and 'O'). The neurotoxic effects of these crude extracts were determined in vivo using the sea shore crab Ocypode macrocera and mortality was observed. The mouse bioassay for lethality was performed on male albino mice. The crude extract of S. mertensii showed higher lethality (58 seconds at 1 mL-dose) than that of S. gigantea (2 minutes and 10 seconds at 0.75 mL-dose). The analgesic activity test was also carried out on albino mice by Eddy's hot plate and tail-flick methods. The extracts showed moderate analgesic effect by both hot-plate and tail-flick methods. These characteristics emphasize the need for the isolation and molecular characterization of new active toxins in S. mertensii and S. gigantea. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 18 1 53 61 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
aqueous extract neurotoxicity mouse bioassay analgesic activity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
aqueous extract neurotoxicity mouse bioassay analgesic activity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 S Thangaraj S Bragadeeswaran Assessment of biomedical and pharmacological activities of sea anemones Stichodactyla mertensii and Stichodactyla gigantea from Gulf of Mannar Biosphere Reserve, southeast coast of India |
topic_facet |
aqueous extract neurotoxicity mouse bioassay analgesic activity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Cnidarians comprise an old and diverse animal phylum, and possess a wide variety of biologically active substances. Sea anemones contain a diversity of interesting biologically active compounds including some potent toxins. In the present work, the sea anemones Stichodactyla mertensii and Stichodactyla gigantea, collected from the Mandapam coast, are characterized biomedically and pharmacologically. The crude protein was obtained by using methanol and aqueous extracts. The respective protein contents of S. mertensii and S. gigantea were found to be 2.10 µg/mL and 1.87 µg/mL. The methanol and aqueous extracts of S. mertensii and S. gigantea yielded six and nine bands by SDS-PAGE on 12% gel. In the hemolytic assay, both extracts exhibited hemolytic effect on chicken, goat, cow and human erythrocytes ('A', 'B' and 'O'). The neurotoxic effects of these crude extracts were determined in vivo using the sea shore crab Ocypode macrocera and mortality was observed. The mouse bioassay for lethality was performed on male albino mice. The crude extract of S. mertensii showed higher lethality (58 seconds at 1 mL-dose) than that of S. gigantea (2 minutes and 10 seconds at 0.75 mL-dose). The analgesic activity test was also carried out on albino mice by Eddy's hot plate and tail-flick methods. The extracts showed moderate analgesic effect by both hot-plate and tail-flick methods. These characteristics emphasize the need for the isolation and molecular characterization of new active toxins in S. mertensii and S. gigantea. |
format |
Article in Journal/Newspaper |
author |
S Thangaraj S Bragadeeswaran |
author_facet |
S Thangaraj S Bragadeeswaran |
author_sort |
S Thangaraj |
title |
Assessment of biomedical and pharmacological activities of sea anemones Stichodactyla mertensii and Stichodactyla gigantea from Gulf of Mannar Biosphere Reserve, southeast coast of India |
title_short |
Assessment of biomedical and pharmacological activities of sea anemones Stichodactyla mertensii and Stichodactyla gigantea from Gulf of Mannar Biosphere Reserve, southeast coast of India |
title_full |
Assessment of biomedical and pharmacological activities of sea anemones Stichodactyla mertensii and Stichodactyla gigantea from Gulf of Mannar Biosphere Reserve, southeast coast of India |
title_fullStr |
Assessment of biomedical and pharmacological activities of sea anemones Stichodactyla mertensii and Stichodactyla gigantea from Gulf of Mannar Biosphere Reserve, southeast coast of India |
title_full_unstemmed |
Assessment of biomedical and pharmacological activities of sea anemones Stichodactyla mertensii and Stichodactyla gigantea from Gulf of Mannar Biosphere Reserve, southeast coast of India |
title_sort |
assessment of biomedical and pharmacological activities of sea anemones stichodactyla mertensii and stichodactyla gigantea from gulf of mannar biosphere reserve, southeast coast of india |
publisher |
SciELO |
publishDate |
2012 |
url |
https://doi.org/10.1590/S1678-91992012000100007 https://doaj.org/article/df6d0b2ca4fe4c9084cce3cfe28d30f9 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 18, Iss 1, Pp 53-61 (2012) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100007 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992012000100007 1678-9199 https://doaj.org/article/df6d0b2ca4fe4c9084cce3cfe28d30f9 |
op_doi |
https://doi.org/10.1590/S1678-91992012000100007 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
18 |
container_issue |
1 |
container_start_page |
53 |
op_container_end_page |
61 |
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