Absence of mutations associated with resistance to benzimidazole in the beta-tubulin gene of Ascaris suum

• Published in: Revista da Sociedade Brasileira de Medicina Tropical
• Main Authors: Adalid Palma, Gabriela Matamoros, Denis Escobar, Ana Lourdes Sánchez, Gustavo Fontecha
• Format: Article in Journal/Newspaper
• Language: English
• Published: Sociedade Brasileira de Medicina Tropical (SBMT) 2020
• Subjects: Ascaris suum; Benzimidazole; Beta-tubulin gene; Arctic medicine. Tropical medicine; RC955-962; Arctic
• Online Access: https://doi.org/10.1590/0037-8682-0155-2019; https://doaj.org/article/df1d58eb723548438b3f72fe50a91346

Abstract INTRODUCTION: Benzimidazoles are commonly used for the control of veterinary nematodes. Resistance to benzimidazoles has been associated with three single nucleotide polymorphisms in the β-tubulin gene of common nematodes. However, these mutations are infrequent in the genus Ascaris spp. METHODS: In order to determine mutations associated with benzimidazole resistance in Ascaris suum, worms were collected from slaughtered pigs and a partial region of the β-tubulin gene was sequenced. RESULTS: All parasites showed the wildtype genotype for codons 167, 198, and 200 of the β-tubulin gene. CONCLUSIONS: This is the first report of genetic sequences associated with benzimidazole resistance in A. suum.