Anaplasma phagocytophilum prevalence in ticks and rodents in an urban and natural habitat in South-Western Slovakia
Abstract Background Ixodes ricinus is the principal vector of Anaplasma phagocytophilum, the ethiological agent of granulocytic anaplasmosis in Europe. Anaplasmosis is an emerging zoonotic disease with a natural enzootic cycle. The reservoir competence of rodents is unclear. Monitoring of A. phagocy...
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ftdoajarticles:oai:doaj.org/article:df153671ef814d9882c7e289a359f3b3 2023-07-02T03:32:57+02:00 Anaplasma phagocytophilum prevalence in ticks and rodents in an urban and natural habitat in South-Western Slovakia Zuzana Svitálková Danka Haruštiaková Lenka Mahríková Lenka Berthová Mirko Slovák Elena Kocianová Mária Kazimírová 2015-05-01T00:00:00Z https://doi.org/10.1186/s13071-015-0880-8 https://doaj.org/article/df153671ef814d9882c7e289a359f3b3 EN eng BMC https://doi.org/10.1186/s13071-015-0880-8 https://doaj.org/toc/1756-3305 doi:10.1186/s13071-015-0880-8 1756-3305 https://doaj.org/article/df153671ef814d9882c7e289a359f3b3 Parasites & Vectors, Vol 8, Iss 1, Pp 1-12 (2015) Anaplasma phagocytophilum Ixodes ricinus Rodents Tick-borne pathogen Infectious and parasitic diseases RC109-216 article 2015 ftdoajarticles https://doi.org/10.1186/s13071-015-0880-8 2023-06-11T00:37:42Z Abstract Background Ixodes ricinus is the principal vector of Anaplasma phagocytophilum, the ethiological agent of granulocytic anaplasmosis in Europe. Anaplasmosis is an emerging zoonotic disease with a natural enzootic cycle. The reservoir competence of rodents is unclear. Monitoring of A. phagocytophilum prevalence in I. ricinus and rodents in various habitat types of Slovakia may contribute to the knowledge about the epidemiology of anaplasmosis in Central Europe. Methods Over 4400 questing ixodid ticks, 1000 rodent-attached ticks and tissue samples of 606 rodents were screened for A. phagocytophilum DNA by real-time PCR targeting the msp2 gene. Ticks and rodents were captured along six transects in an urban/suburban and natural habitat in south-western Slovakia during 2011–2014. Estimates of wildlife (roe deer, red deer, fallow deer, mouflon, wild boar) densities in the study area were taken from hunter’s yearly reports. Spatial and temporal differences in A. phagocytophilum prevalence in questing I. ricinus and relationships with relative abundance of ticks and wildlife were analysed. Results Overall prevalence of A. phagocytophilum in questing I. ricinus was significantly higher in the urban/suburban habitat (7.2 %; 95 % CI: 6.1–8.3 %) compared to the natural habitat (3.1 %; 95 % CI: 2.5–3.9 %) (χ 2 = 37.451; P < 0.001). Significant local differences in prevalence of infected questing ticks were found among transects within each habitat as well as among years and between seasons. The trapped rodents belonged to six species. Apodemus flavicollis and Myodes glareolus prevailed in both habitats, Microtus arvalis was present only in the natural habitat. I. ricinus comprised 96.3 % of the rodent-attached ticks, the rest were Haemaphysalis concinna, Ixodes trianguliceps and Dermacentor reticulatus. Only 0.5 % of rodent skin and 0.6 % of rodent-attached ticks (only I. ricinus) were infected with A. phagocytophilum. Prevalence of A. phagocytophilum in questing I. ricinus did not correlate significantly with ... Article in Journal/Newspaper Microtus arvalis Directory of Open Access Journals: DOAJ Articles Parasites & Vectors 8 1 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Anaplasma phagocytophilum Ixodes ricinus Rodents Tick-borne pathogen Infectious and parasitic diseases RC109-216 |
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Anaplasma phagocytophilum Ixodes ricinus Rodents Tick-borne pathogen Infectious and parasitic diseases RC109-216 Zuzana Svitálková Danka Haruštiaková Lenka Mahríková Lenka Berthová Mirko Slovák Elena Kocianová Mária Kazimírová Anaplasma phagocytophilum prevalence in ticks and rodents in an urban and natural habitat in South-Western Slovakia |
topic_facet |
Anaplasma phagocytophilum Ixodes ricinus Rodents Tick-borne pathogen Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Ixodes ricinus is the principal vector of Anaplasma phagocytophilum, the ethiological agent of granulocytic anaplasmosis in Europe. Anaplasmosis is an emerging zoonotic disease with a natural enzootic cycle. The reservoir competence of rodents is unclear. Monitoring of A. phagocytophilum prevalence in I. ricinus and rodents in various habitat types of Slovakia may contribute to the knowledge about the epidemiology of anaplasmosis in Central Europe. Methods Over 4400 questing ixodid ticks, 1000 rodent-attached ticks and tissue samples of 606 rodents were screened for A. phagocytophilum DNA by real-time PCR targeting the msp2 gene. Ticks and rodents were captured along six transects in an urban/suburban and natural habitat in south-western Slovakia during 2011–2014. Estimates of wildlife (roe deer, red deer, fallow deer, mouflon, wild boar) densities in the study area were taken from hunter’s yearly reports. Spatial and temporal differences in A. phagocytophilum prevalence in questing I. ricinus and relationships with relative abundance of ticks and wildlife were analysed. Results Overall prevalence of A. phagocytophilum in questing I. ricinus was significantly higher in the urban/suburban habitat (7.2 %; 95 % CI: 6.1–8.3 %) compared to the natural habitat (3.1 %; 95 % CI: 2.5–3.9 %) (χ 2 = 37.451; P < 0.001). Significant local differences in prevalence of infected questing ticks were found among transects within each habitat as well as among years and between seasons. The trapped rodents belonged to six species. Apodemus flavicollis and Myodes glareolus prevailed in both habitats, Microtus arvalis was present only in the natural habitat. I. ricinus comprised 96.3 % of the rodent-attached ticks, the rest were Haemaphysalis concinna, Ixodes trianguliceps and Dermacentor reticulatus. Only 0.5 % of rodent skin and 0.6 % of rodent-attached ticks (only I. ricinus) were infected with A. phagocytophilum. Prevalence of A. phagocytophilum in questing I. ricinus did not correlate significantly with ... |
format |
Article in Journal/Newspaper |
author |
Zuzana Svitálková Danka Haruštiaková Lenka Mahríková Lenka Berthová Mirko Slovák Elena Kocianová Mária Kazimírová |
author_facet |
Zuzana Svitálková Danka Haruštiaková Lenka Mahríková Lenka Berthová Mirko Slovák Elena Kocianová Mária Kazimírová |
author_sort |
Zuzana Svitálková |
title |
Anaplasma phagocytophilum prevalence in ticks and rodents in an urban and natural habitat in South-Western Slovakia |
title_short |
Anaplasma phagocytophilum prevalence in ticks and rodents in an urban and natural habitat in South-Western Slovakia |
title_full |
Anaplasma phagocytophilum prevalence in ticks and rodents in an urban and natural habitat in South-Western Slovakia |
title_fullStr |
Anaplasma phagocytophilum prevalence in ticks and rodents in an urban and natural habitat in South-Western Slovakia |
title_full_unstemmed |
Anaplasma phagocytophilum prevalence in ticks and rodents in an urban and natural habitat in South-Western Slovakia |
title_sort |
anaplasma phagocytophilum prevalence in ticks and rodents in an urban and natural habitat in south-western slovakia |
publisher |
BMC |
publishDate |
2015 |
url |
https://doi.org/10.1186/s13071-015-0880-8 https://doaj.org/article/df153671ef814d9882c7e289a359f3b3 |
genre |
Microtus arvalis |
genre_facet |
Microtus arvalis |
op_source |
Parasites & Vectors, Vol 8, Iss 1, Pp 1-12 (2015) |
op_relation |
https://doi.org/10.1186/s13071-015-0880-8 https://doaj.org/toc/1756-3305 doi:10.1186/s13071-015-0880-8 1756-3305 https://doaj.org/article/df153671ef814d9882c7e289a359f3b3 |
op_doi |
https://doi.org/10.1186/s13071-015-0880-8 |
container_title |
Parasites & Vectors |
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8 |
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1 |
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1770272657536712704 |