Molecular detection of Trypanosoma sp. and Blastocrithidia sp. (Trypanosomatidae) in phlebotomine sand flies (Psychodidae) in the Federal District of Brazil

• Published in: Revista da Sociedade Brasileira de Medicina Tropical
• Main Authors: Tauana de Sousa Ferreira, Thaís Tâmara Castro Minuzzi-Souza, Andrey José de Andrade, Thais Oliveira Coelho, Douglas de Almeida Rocha, Marcos Takashi Obara, Mariana Hecht, Nadjar Nitz, Rodrigo Gurgel-Gonçalves
• Format: Article in Journal/Newspaper
• Language: English
• Published: Sociedade Brasileira de Medicina Tropical (SBMT) 2015
• Subjects: PCR; Trypanosomatids; Phlebotominae; Arctic medicine. Tropical medicine; RC955-962; Arctic
• Online Access: https://doi.org/10.1590/0037-8682-0076-2015; https://doaj.org/article/df01b2a0ba944971b493bbacc4ccc256

Abstract: INTRODUCTION : This study describes the occurrence of trypanosomatids in phlebotomines in Brasília, Brazil. METHODS : Two hundred and ten females of 13 sand fly species were analyzed by polymerase chain reaction (PCR) using different molecular markers (D7 24Sα rRNA, kDNA, and ITS1) and sequencing. RESULTS : PCR revealed trypanosomatid-positive samples from Nyssomyia whitmani and Evandromyia evandroi, which were negative by kDNA and ITS1 Leishmania-specific PCRs. DNA sequence analysis of D7 24Sα rRNA amplicons indicated the occurrence of Blastocrithidia sp. and Trypanosoma sp. in Nyssomyia whitmani and Evandromyia evandroi, respectively. CONCLUSIONS : Two trypanosomatid species other than Leishmania sp. were found to circulate in sand flies in Central Brazil.