Apoptosis stalks Plasmodium falciparum maintained in continuous culture condition
Abstract Background Growth kinetic of Plasmodium falciparum in culture or in the host fall short of expected growth rate considering that there are 4 x 10 6 /µL red blood cell (RBCs) available for invasion and about 16 merozoites growing in each infected RBC. This study determined whether apoptotic...
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ftdoajarticles:oai:doaj.org/article:deb37522e05747df964aa0aa8d7c4845 2023-05-15T15:09:40+02:00 Apoptosis stalks Plasmodium falciparum maintained in continuous culture condition Waitumbi John N Mutai Beth K 2010-12-01T00:00:00Z https://doi.org/10.1186/1475-2875-9-S3-S6 https://doaj.org/article/deb37522e05747df964aa0aa8d7c4845 EN eng BMC https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-S3-S6 1475-2875 https://doaj.org/article/deb37522e05747df964aa0aa8d7c4845 Malaria Journal, Vol 9, Iss Suppl 3, p S6 (2010) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2010 ftdoajarticles https://doi.org/10.1186/1475-2875-9-S3-S6 2022-12-30T22:45:55Z Abstract Background Growth kinetic of Plasmodium falciparum in culture or in the host fall short of expected growth rate considering that there are 4 x 10 6 /µL red blood cell (RBCs) available for invasion and about 16 merozoites growing in each infected RBC. This study determined whether apoptotic machinery is operable to keep the parasite population under check. Methods A synchronized culture of P. falciparum (Dd2 strain) was initiated at 0.5% ring stage parasitaemia and kept under conditions not limiting for RBCs and nutrient by adjusting hematocrit to 5% at each schizogony and changing growth media daily. Parasite growth pattern and morphology was evaluated by blood smear microscopy and flow-cytometry using SYBR green. The apoptotic processes were evaluated for evidence of: DNA fragmentation by TUNEL, collapse of mitochondria membrane potential (ΔΨ m ) by TMRE, expression of metacaspse gene by RT-qPCR and by probing parasite proteins with anti-caspase antibodies. Results From the seeding parasitaemia of 0.5%, the parasites doubled every 48 hours to a parasitaemia of 4%. Thereafter, the growth stagnated and the culture consistently crashed at about 6% parasitaemia. ΔΨ m potential collapsed as the parasite density increased and DNA fragmentation increased steadily from 0.2% to ~6%. The expression of metacaspase gene and protein was observed in all stages, but their abundance was variable among the stages. Conclusion These findings suggest existence of P. falciparum quorum sensing that keep the parasite population under check. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 9 S3 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Waitumbi John N Mutai Beth K Apoptosis stalks Plasmodium falciparum maintained in continuous culture condition |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Growth kinetic of Plasmodium falciparum in culture or in the host fall short of expected growth rate considering that there are 4 x 10 6 /µL red blood cell (RBCs) available for invasion and about 16 merozoites growing in each infected RBC. This study determined whether apoptotic machinery is operable to keep the parasite population under check. Methods A synchronized culture of P. falciparum (Dd2 strain) was initiated at 0.5% ring stage parasitaemia and kept under conditions not limiting for RBCs and nutrient by adjusting hematocrit to 5% at each schizogony and changing growth media daily. Parasite growth pattern and morphology was evaluated by blood smear microscopy and flow-cytometry using SYBR green. The apoptotic processes were evaluated for evidence of: DNA fragmentation by TUNEL, collapse of mitochondria membrane potential (ΔΨ m ) by TMRE, expression of metacaspse gene by RT-qPCR and by probing parasite proteins with anti-caspase antibodies. Results From the seeding parasitaemia of 0.5%, the parasites doubled every 48 hours to a parasitaemia of 4%. Thereafter, the growth stagnated and the culture consistently crashed at about 6% parasitaemia. ΔΨ m potential collapsed as the parasite density increased and DNA fragmentation increased steadily from 0.2% to ~6%. The expression of metacaspase gene and protein was observed in all stages, but their abundance was variable among the stages. Conclusion These findings suggest existence of P. falciparum quorum sensing that keep the parasite population under check. |
format |
Article in Journal/Newspaper |
author |
Waitumbi John N Mutai Beth K |
author_facet |
Waitumbi John N Mutai Beth K |
author_sort |
Waitumbi John N |
title |
Apoptosis stalks Plasmodium falciparum maintained in continuous culture condition |
title_short |
Apoptosis stalks Plasmodium falciparum maintained in continuous culture condition |
title_full |
Apoptosis stalks Plasmodium falciparum maintained in continuous culture condition |
title_fullStr |
Apoptosis stalks Plasmodium falciparum maintained in continuous culture condition |
title_full_unstemmed |
Apoptosis stalks Plasmodium falciparum maintained in continuous culture condition |
title_sort |
apoptosis stalks plasmodium falciparum maintained in continuous culture condition |
publisher |
BMC |
publishDate |
2010 |
url |
https://doi.org/10.1186/1475-2875-9-S3-S6 https://doaj.org/article/deb37522e05747df964aa0aa8d7c4845 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 9, Iss Suppl 3, p S6 (2010) |
op_relation |
https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-S3-S6 1475-2875 https://doaj.org/article/deb37522e05747df964aa0aa8d7c4845 |
op_doi |
https://doi.org/10.1186/1475-2875-9-S3-S6 |
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Malaria Journal |
container_volume |
9 |
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S3 |
_version_ |
1766340813681328128 |