Genetic diversity of human respiratory syncytial virus isolated among children with acute respiratory infections in Southern Cameroon during three consecutive epidemic seasons, 2011–2013
Abstract Background Human respiratory syncytial virus (HRSV) is the main viral cause of severe lower respiratory tract disease in infants and young children. The aim of this study was to describe for the first time the genetic variability of HRSV in Cameroonian patients living in Yaounde for three c...
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ftdoajarticles:oai:doaj.org/article:de9ed8637eac4124a0282da1504162c0 2023-05-15T15:09:35+02:00 Genetic diversity of human respiratory syncytial virus isolated among children with acute respiratory infections in Southern Cameroon during three consecutive epidemic seasons, 2011–2013 Sebastien Kenmoe Marie-Astrid Vernet Fabien Miszczak Julia Dina Matthieu Schoenhals Véronique Penlap Beng Astrid Vabret Richard Njouom 2018-04-01T00:00:00Z https://doi.org/10.1186/s41182-018-0088-7 https://doaj.org/article/de9ed8637eac4124a0282da1504162c0 EN eng BMC http://link.springer.com/article/10.1186/s41182-018-0088-7 https://doaj.org/toc/1349-4147 doi:10.1186/s41182-018-0088-7 1349-4147 https://doaj.org/article/de9ed8637eac4124a0282da1504162c0 Tropical Medicine and Health, Vol 46, Iss 1, Pp 1-8 (2018) Cameroon Genetic characterization Human respiratory syncytial virus Molecular epidemiology Arctic medicine. Tropical medicine RC955-962 article 2018 ftdoajarticles https://doi.org/10.1186/s41182-018-0088-7 2022-12-31T00:17:43Z Abstract Background Human respiratory syncytial virus (HRSV) is the main viral cause of severe lower respiratory tract disease in infants and young children. The aim of this study was to describe for the first time the genetic variability of HRSV in Cameroonian patients living in Yaounde for three consecutive epidemic seasons. Methods HRSV-positive nasopharyngeal samples detected in children less than 15 years in Yaounde were collected from September 2011 to December 2013. Semi-nested RT-PCR, sequencing, and phylogenetic analyses of the second hypervariable region of the G gene were performed. Results A total of 57 HRSV-positive samples were collected during the study period. Among these, 46 (80.7%) could be amplified in the G gene. HRSV group A (HRSV-A) and group B (HRSV-B) co-circulated in this population at 17.4 and 82.6%, respectively. HRSV-A strains clustered in the NA-1 genotype while HRSV-B strains clustered in the BA-9 genotype. HRSV-A strains accounted for 33.3% (2/6), 4.3% (1/23), and 29.4% (5/17) of the viruses isolated in 2011, 2012, and 2013, respectively. Conclusions This study reports molecular epidemiology data of HRSV in Cameroon for the first time. Additional studies are required to clarify evolutionary patterns of HRSV throughout sub-Saharan Africa to support antiviral and vaccine development. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Tropical Medicine and Health 46 1 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Cameroon Genetic characterization Human respiratory syncytial virus Molecular epidemiology Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Cameroon Genetic characterization Human respiratory syncytial virus Molecular epidemiology Arctic medicine. Tropical medicine RC955-962 Sebastien Kenmoe Marie-Astrid Vernet Fabien Miszczak Julia Dina Matthieu Schoenhals Véronique Penlap Beng Astrid Vabret Richard Njouom Genetic diversity of human respiratory syncytial virus isolated among children with acute respiratory infections in Southern Cameroon during three consecutive epidemic seasons, 2011–2013 |
topic_facet |
Cameroon Genetic characterization Human respiratory syncytial virus Molecular epidemiology Arctic medicine. Tropical medicine RC955-962 |
description |
Abstract Background Human respiratory syncytial virus (HRSV) is the main viral cause of severe lower respiratory tract disease in infants and young children. The aim of this study was to describe for the first time the genetic variability of HRSV in Cameroonian patients living in Yaounde for three consecutive epidemic seasons. Methods HRSV-positive nasopharyngeal samples detected in children less than 15 years in Yaounde were collected from September 2011 to December 2013. Semi-nested RT-PCR, sequencing, and phylogenetic analyses of the second hypervariable region of the G gene were performed. Results A total of 57 HRSV-positive samples were collected during the study period. Among these, 46 (80.7%) could be amplified in the G gene. HRSV group A (HRSV-A) and group B (HRSV-B) co-circulated in this population at 17.4 and 82.6%, respectively. HRSV-A strains clustered in the NA-1 genotype while HRSV-B strains clustered in the BA-9 genotype. HRSV-A strains accounted for 33.3% (2/6), 4.3% (1/23), and 29.4% (5/17) of the viruses isolated in 2011, 2012, and 2013, respectively. Conclusions This study reports molecular epidemiology data of HRSV in Cameroon for the first time. Additional studies are required to clarify evolutionary patterns of HRSV throughout sub-Saharan Africa to support antiviral and vaccine development. |
format |
Article in Journal/Newspaper |
author |
Sebastien Kenmoe Marie-Astrid Vernet Fabien Miszczak Julia Dina Matthieu Schoenhals Véronique Penlap Beng Astrid Vabret Richard Njouom |
author_facet |
Sebastien Kenmoe Marie-Astrid Vernet Fabien Miszczak Julia Dina Matthieu Schoenhals Véronique Penlap Beng Astrid Vabret Richard Njouom |
author_sort |
Sebastien Kenmoe |
title |
Genetic diversity of human respiratory syncytial virus isolated among children with acute respiratory infections in Southern Cameroon during three consecutive epidemic seasons, 2011–2013 |
title_short |
Genetic diversity of human respiratory syncytial virus isolated among children with acute respiratory infections in Southern Cameroon during three consecutive epidemic seasons, 2011–2013 |
title_full |
Genetic diversity of human respiratory syncytial virus isolated among children with acute respiratory infections in Southern Cameroon during three consecutive epidemic seasons, 2011–2013 |
title_fullStr |
Genetic diversity of human respiratory syncytial virus isolated among children with acute respiratory infections in Southern Cameroon during three consecutive epidemic seasons, 2011–2013 |
title_full_unstemmed |
Genetic diversity of human respiratory syncytial virus isolated among children with acute respiratory infections in Southern Cameroon during three consecutive epidemic seasons, 2011–2013 |
title_sort |
genetic diversity of human respiratory syncytial virus isolated among children with acute respiratory infections in southern cameroon during three consecutive epidemic seasons, 2011–2013 |
publisher |
BMC |
publishDate |
2018 |
url |
https://doi.org/10.1186/s41182-018-0088-7 https://doaj.org/article/de9ed8637eac4124a0282da1504162c0 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Tropical Medicine and Health, Vol 46, Iss 1, Pp 1-8 (2018) |
op_relation |
http://link.springer.com/article/10.1186/s41182-018-0088-7 https://doaj.org/toc/1349-4147 doi:10.1186/s41182-018-0088-7 1349-4147 https://doaj.org/article/de9ed8637eac4124a0282da1504162c0 |
op_doi |
https://doi.org/10.1186/s41182-018-0088-7 |
container_title |
Tropical Medicine and Health |
container_volume |
46 |
container_issue |
1 |
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1766340744234139648 |