Influenza virus infection and postviral bacterial pneumonia pathogenesis induced by different subtypes of influenza virus in mice
Secondary bacterial infections after influenza virus infection further increase morbidity and mortality due to influenza. Despite of seasonal influenza vaccination, antiviral drugs and antibiotics are widely used in viral/bacterial pneumonia therapy. Therefore, further comprehensive study of the inf...
Published in: | Medical Immunology (Russia) |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | Russian |
Published: |
St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2020
|
Subjects: | |
Online Access: | https://doi.org/10.15789/1563-0625-IVI-1840 https://doaj.org/article/de8b54f6a44a4adf9274be541b89aa58 |
id |
ftdoajarticles:oai:doaj.org/article:de8b54f6a44a4adf9274be541b89aa58 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:de8b54f6a44a4adf9274be541b89aa58 2024-09-15T17:39:17+00:00 Influenza virus infection and postviral bacterial pneumonia pathogenesis induced by different subtypes of influenza virus in mice A. A. Poromov N. R. Makhmudova I. N. Falynskova E. A. Glubokova N. P. Kartashova I. T. Fedyakina O. A. Svitich I. A. Leneva 2020-01-01T00:00:00Z https://doi.org/10.15789/1563-0625-IVI-1840 https://doaj.org/article/de8b54f6a44a4adf9274be541b89aa58 RU rus St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists https://www.mimmun.ru/mimmun/article/view/1840 https://doaj.org/toc/1563-0625 https://doaj.org/toc/2313-741X 1563-0625 2313-741X doi:10.15789/1563-0625-IVI-1840 https://doaj.org/article/de8b54f6a44a4adf9274be541b89aa58 Медицинская иммунология, Vol 22, Iss 1, Pp 99-110 (2020) influenza staphylococcus aureus secondary bacterial pneumonia oseltamivr cefuroxime cytokine Immunologic diseases. Allergy RC581-607 article 2020 ftdoajarticles https://doi.org/10.15789/1563-0625-IVI-1840 2024-08-05T17:48:49Z Secondary bacterial infections after influenza virus infection further increase morbidity and mortality due to influenza. Despite of seasonal influenza vaccination, antiviral drugs and antibiotics are widely used in viral/bacterial pneumonia therapy. Therefore, further comprehensive study of the infection pathogenesis is relevant. Murine models for influenza virus infection were reproduced with different virus subtypes A/California/04/2009MA (pandemic H1N1 2009), A/Puerto Rico/8/34 (H1N1) and A/Aichi/2/69 (H3N2), Anadyr/177/2009 (H1N1) and for post-influenza bacterial pneumonia caused by the Gram-positive Staphylococcus aureus. After the infection occurs, its pathogenic features were detected by daily monitoring the mortality (survival) and morbidity rate (body weight loss) and, in addition, viral pathogenesis also was evaluated by assessing virus replication (viral titer) and humoral immune responses (production of pro- and anti-inflammatory cytokines) in respiratory tract of infected mice including during antiviral (oseltamivir) and antibacterial (cefuroxime) therapy. Mortality and virus titer in the infected mice did not differ significantly between the groups of different influenza A virus subtypes. However, production of cytokines (IL-10, IFNg, TNFa) and weight gain proved to be different. Mortality of the mice reached 100% after secondary bacterial infection, whereas IFNg and TNFa levels in mice lung increased reached maximal values in the treated groups. Viral subtype A/California/04/2009MA of influenza A was most pathogenic in mouse model of secondary bacterial pneumonia. Antiviral and antibacterial treatment caused a decrease in mortality, reduced viral titers in lungs, and retain body weight gain of mice. According to these points, the treatment groups did not significantly differ from each other. At the same time, it should be noted that the cytokine production significantly decreased in the treated groups, and IL-10 and IFNg levels in lungs were different, that may be due to therapeutic mechanisms of ... Article in Journal/Newspaper Anadyr Anadyr' Directory of Open Access Journals: DOAJ Articles Medical Immunology (Russia) 22 1 99 110 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
Russian |
topic |
influenza staphylococcus aureus secondary bacterial pneumonia oseltamivr cefuroxime cytokine Immunologic diseases. Allergy RC581-607 |
spellingShingle |
influenza staphylococcus aureus secondary bacterial pneumonia oseltamivr cefuroxime cytokine Immunologic diseases. Allergy RC581-607 A. A. Poromov N. R. Makhmudova I. N. Falynskova E. A. Glubokova N. P. Kartashova I. T. Fedyakina O. A. Svitich I. A. Leneva Influenza virus infection and postviral bacterial pneumonia pathogenesis induced by different subtypes of influenza virus in mice |
topic_facet |
influenza staphylococcus aureus secondary bacterial pneumonia oseltamivr cefuroxime cytokine Immunologic diseases. Allergy RC581-607 |
description |
Secondary bacterial infections after influenza virus infection further increase morbidity and mortality due to influenza. Despite of seasonal influenza vaccination, antiviral drugs and antibiotics are widely used in viral/bacterial pneumonia therapy. Therefore, further comprehensive study of the infection pathogenesis is relevant. Murine models for influenza virus infection were reproduced with different virus subtypes A/California/04/2009MA (pandemic H1N1 2009), A/Puerto Rico/8/34 (H1N1) and A/Aichi/2/69 (H3N2), Anadyr/177/2009 (H1N1) and for post-influenza bacterial pneumonia caused by the Gram-positive Staphylococcus aureus. After the infection occurs, its pathogenic features were detected by daily monitoring the mortality (survival) and morbidity rate (body weight loss) and, in addition, viral pathogenesis also was evaluated by assessing virus replication (viral titer) and humoral immune responses (production of pro- and anti-inflammatory cytokines) in respiratory tract of infected mice including during antiviral (oseltamivir) and antibacterial (cefuroxime) therapy. Mortality and virus titer in the infected mice did not differ significantly between the groups of different influenza A virus subtypes. However, production of cytokines (IL-10, IFNg, TNFa) and weight gain proved to be different. Mortality of the mice reached 100% after secondary bacterial infection, whereas IFNg and TNFa levels in mice lung increased reached maximal values in the treated groups. Viral subtype A/California/04/2009MA of influenza A was most pathogenic in mouse model of secondary bacterial pneumonia. Antiviral and antibacterial treatment caused a decrease in mortality, reduced viral titers in lungs, and retain body weight gain of mice. According to these points, the treatment groups did not significantly differ from each other. At the same time, it should be noted that the cytokine production significantly decreased in the treated groups, and IL-10 and IFNg levels in lungs were different, that may be due to therapeutic mechanisms of ... |
format |
Article in Journal/Newspaper |
author |
A. A. Poromov N. R. Makhmudova I. N. Falynskova E. A. Glubokova N. P. Kartashova I. T. Fedyakina O. A. Svitich I. A. Leneva |
author_facet |
A. A. Poromov N. R. Makhmudova I. N. Falynskova E. A. Glubokova N. P. Kartashova I. T. Fedyakina O. A. Svitich I. A. Leneva |
author_sort |
A. A. Poromov |
title |
Influenza virus infection and postviral bacterial pneumonia pathogenesis induced by different subtypes of influenza virus in mice |
title_short |
Influenza virus infection and postviral bacterial pneumonia pathogenesis induced by different subtypes of influenza virus in mice |
title_full |
Influenza virus infection and postviral bacterial pneumonia pathogenesis induced by different subtypes of influenza virus in mice |
title_fullStr |
Influenza virus infection and postviral bacterial pneumonia pathogenesis induced by different subtypes of influenza virus in mice |
title_full_unstemmed |
Influenza virus infection and postviral bacterial pneumonia pathogenesis induced by different subtypes of influenza virus in mice |
title_sort |
influenza virus infection and postviral bacterial pneumonia pathogenesis induced by different subtypes of influenza virus in mice |
publisher |
St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
publishDate |
2020 |
url |
https://doi.org/10.15789/1563-0625-IVI-1840 https://doaj.org/article/de8b54f6a44a4adf9274be541b89aa58 |
genre |
Anadyr Anadyr' |
genre_facet |
Anadyr Anadyr' |
op_source |
Медицинская иммунология, Vol 22, Iss 1, Pp 99-110 (2020) |
op_relation |
https://www.mimmun.ru/mimmun/article/view/1840 https://doaj.org/toc/1563-0625 https://doaj.org/toc/2313-741X 1563-0625 2313-741X doi:10.15789/1563-0625-IVI-1840 https://doaj.org/article/de8b54f6a44a4adf9274be541b89aa58 |
op_doi |
https://doi.org/10.15789/1563-0625-IVI-1840 |
container_title |
Medical Immunology (Russia) |
container_volume |
22 |
container_issue |
1 |
container_start_page |
99 |
op_container_end_page |
110 |
_version_ |
1810478853009702912 |