An alternative method for correcting fluorescence quenching
Under high light intensity, phytoplankton protect their photosystems from bleaching through non-photochemical quenching processes. The consequence of this is suppression of fluorescence emission, which must be corrected when measuring in situ yield with fluorometers. We present data from the Souther...
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ftdoajarticles:oai:doaj.org/article:de73be1d08094651abc76b4573a52dfc 2023-05-15T16:05:43+02:00 An alternative method for correcting fluorescence quenching L. Biermann C. Guinet M. Bester A. Brierley L. Boehme 2015-01-01T00:00:00Z https://doi.org/10.5194/os-11-83-2015 https://doaj.org/article/de73be1d08094651abc76b4573a52dfc EN eng Copernicus Publications http://www.ocean-sci.net/11/83/2015/os-11-83-2015.pdf https://doaj.org/toc/1812-0784 https://doaj.org/toc/1812-0792 1812-0784 1812-0792 doi:10.5194/os-11-83-2015 https://doaj.org/article/de73be1d08094651abc76b4573a52dfc Ocean Science, Vol 11, Iss 1, Pp 83-91 (2015) Geography. Anthropology. Recreation G Environmental sciences GE1-350 article 2015 ftdoajarticles https://doi.org/10.5194/os-11-83-2015 2022-12-30T20:49:03Z Under high light intensity, phytoplankton protect their photosystems from bleaching through non-photochemical quenching processes. The consequence of this is suppression of fluorescence emission, which must be corrected when measuring in situ yield with fluorometers. We present data from the Southern Ocean, collected over five austral summers by 19 southern elephant seals tagged with fluorometers. Conventionally, fluorescence data collected during the day (quenched) were corrected using the limit of the mixed layer, assuming that phytoplankton are uniformly mixed from the surface to this depth. However, distinct deep fluorescence maxima were measured in approximately 30% of the night (unquenched) data. To account for the evidence that chlorophyll is not uniformly mixed in the upper layer, we propose correcting from the limit of the euphotic zone, defined as the depth at which photosynthetically available radiation is ~ 1% of the surface value. Mixed layer depth exceeded euphotic depth over 80% of the time. Under these conditions, quenching was corrected from the depth of the remotely derived euphotic zone Z eu , and compared with fluorescence corrected from the depth of the density-derived mixed layer. Deep fluorescence maxima were evident in only 10% of the day data when correcting from mixed layer depth. This was doubled to 21% when correcting from Z eu , more closely matching the unquenched (night) data. Furthermore, correcting from Z eu served to conserve non-uniform chlorophyll features found between the 1% light level and mixed layer depth. Article in Journal/Newspaper Elephant Seals Southern Elephant Seals Southern Ocean Directory of Open Access Journals: DOAJ Articles Austral Southern Ocean Ocean Science 11 1 83 91 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Geography. Anthropology. Recreation G Environmental sciences GE1-350 |
spellingShingle |
Geography. Anthropology. Recreation G Environmental sciences GE1-350 L. Biermann C. Guinet M. Bester A. Brierley L. Boehme An alternative method for correcting fluorescence quenching |
topic_facet |
Geography. Anthropology. Recreation G Environmental sciences GE1-350 |
description |
Under high light intensity, phytoplankton protect their photosystems from bleaching through non-photochemical quenching processes. The consequence of this is suppression of fluorescence emission, which must be corrected when measuring in situ yield with fluorometers. We present data from the Southern Ocean, collected over five austral summers by 19 southern elephant seals tagged with fluorometers. Conventionally, fluorescence data collected during the day (quenched) were corrected using the limit of the mixed layer, assuming that phytoplankton are uniformly mixed from the surface to this depth. However, distinct deep fluorescence maxima were measured in approximately 30% of the night (unquenched) data. To account for the evidence that chlorophyll is not uniformly mixed in the upper layer, we propose correcting from the limit of the euphotic zone, defined as the depth at which photosynthetically available radiation is ~ 1% of the surface value. Mixed layer depth exceeded euphotic depth over 80% of the time. Under these conditions, quenching was corrected from the depth of the remotely derived euphotic zone Z eu , and compared with fluorescence corrected from the depth of the density-derived mixed layer. Deep fluorescence maxima were evident in only 10% of the day data when correcting from mixed layer depth. This was doubled to 21% when correcting from Z eu , more closely matching the unquenched (night) data. Furthermore, correcting from Z eu served to conserve non-uniform chlorophyll features found between the 1% light level and mixed layer depth. |
format |
Article in Journal/Newspaper |
author |
L. Biermann C. Guinet M. Bester A. Brierley L. Boehme |
author_facet |
L. Biermann C. Guinet M. Bester A. Brierley L. Boehme |
author_sort |
L. Biermann |
title |
An alternative method for correcting fluorescence quenching |
title_short |
An alternative method for correcting fluorescence quenching |
title_full |
An alternative method for correcting fluorescence quenching |
title_fullStr |
An alternative method for correcting fluorescence quenching |
title_full_unstemmed |
An alternative method for correcting fluorescence quenching |
title_sort |
alternative method for correcting fluorescence quenching |
publisher |
Copernicus Publications |
publishDate |
2015 |
url |
https://doi.org/10.5194/os-11-83-2015 https://doaj.org/article/de73be1d08094651abc76b4573a52dfc |
geographic |
Austral Southern Ocean |
geographic_facet |
Austral Southern Ocean |
genre |
Elephant Seals Southern Elephant Seals Southern Ocean |
genre_facet |
Elephant Seals Southern Elephant Seals Southern Ocean |
op_source |
Ocean Science, Vol 11, Iss 1, Pp 83-91 (2015) |
op_relation |
http://www.ocean-sci.net/11/83/2015/os-11-83-2015.pdf https://doaj.org/toc/1812-0784 https://doaj.org/toc/1812-0792 1812-0784 1812-0792 doi:10.5194/os-11-83-2015 https://doaj.org/article/de73be1d08094651abc76b4573a52dfc |
op_doi |
https://doi.org/10.5194/os-11-83-2015 |
container_title |
Ocean Science |
container_volume |
11 |
container_issue |
1 |
container_start_page |
83 |
op_container_end_page |
91 |
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1766401621161410560 |