Validation of oral fluid samples to monitor serological changes to Plasmodium falciparum : An observational study in southern Zambia
Abstract Background In formerly endemic areas where malaria transmission has declined, levels of population immunity to Plasmodium falciparum provide information on continued malaria transmission and potentially susceptible populations. Traditional techniques for measuring serological responses to P...
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ftdoajarticles:oai:doaj.org/article:de4e815bb83f4af1957faac2437f8f28 2023-05-15T15:10:45+02:00 Validation of oral fluid samples to monitor serological changes to Plasmodium falciparum : An observational study in southern Zambia Mharakurwa Sungano Hamapumbu Harry Kobayashi Tamaki Chishimba Sandra Chidi Alexis P Thuma Philip E Moss William J 2011-06-01T00:00:00Z https://doi.org/10.1186/1475-2875-10-162 https://doaj.org/article/de4e815bb83f4af1957faac2437f8f28 EN eng BMC http://www.malariajournal.com/content/10/1/162 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-10-162 1475-2875 https://doaj.org/article/de4e815bb83f4af1957faac2437f8f28 Malaria Journal, Vol 10, Iss 1, p 162 (2011) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2011 ftdoajarticles https://doi.org/10.1186/1475-2875-10-162 2022-12-30T21:40:16Z Abstract Background In formerly endemic areas where malaria transmission has declined, levels of population immunity to Plasmodium falciparum provide information on continued malaria transmission and potentially susceptible populations. Traditional techniques for measuring serological responses to P. falciparum antigens use plasma or dried blood spots (DBS). These invasive procedures pose a biohazard and may be unacceptable to communities if performed frequently. The use of oral fluid (OF) samples to detect antibodies to P. falciparum antigens may be a more acceptable strategy to monitor changes in population immunity. Methods An enzyme immunoassay was optimized to detect antibodies to whole, asexual stage P. falciparum antigens. Optical density (OD) values from paired DBS and OF samples collected as part of a community-based survey of malaria parasitaemia were compared. Results Oral fluid and dried blood spot samples were collected from 53 participants in Southern Province, Zambia. Their ages ranged from 1 to 80 years and 45% were female. A statistically significant correlation (r = 0.79; P < 0.01) was observed between OD values from OF and DBS samples. The OF assay identified all DBS-confirmed positive and negative samples, resulting in 100% sensitivity and specificity. Conclusions Oral fluid is a valid alternative specimen for monitoring changes in antibodies to P. falciparum antigens. As OF collection is often more acceptable to communities, poses less of a biohazard than blood samples and can be performed by community volunteers, serological surveys using OF samples provide a strategy for monitoring population immunity in regions of declining malaria transmission. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 10 1 162 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Mharakurwa Sungano Hamapumbu Harry Kobayashi Tamaki Chishimba Sandra Chidi Alexis P Thuma Philip E Moss William J Validation of oral fluid samples to monitor serological changes to Plasmodium falciparum : An observational study in southern Zambia |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background In formerly endemic areas where malaria transmission has declined, levels of population immunity to Plasmodium falciparum provide information on continued malaria transmission and potentially susceptible populations. Traditional techniques for measuring serological responses to P. falciparum antigens use plasma or dried blood spots (DBS). These invasive procedures pose a biohazard and may be unacceptable to communities if performed frequently. The use of oral fluid (OF) samples to detect antibodies to P. falciparum antigens may be a more acceptable strategy to monitor changes in population immunity. Methods An enzyme immunoassay was optimized to detect antibodies to whole, asexual stage P. falciparum antigens. Optical density (OD) values from paired DBS and OF samples collected as part of a community-based survey of malaria parasitaemia were compared. Results Oral fluid and dried blood spot samples were collected from 53 participants in Southern Province, Zambia. Their ages ranged from 1 to 80 years and 45% were female. A statistically significant correlation (r = 0.79; P < 0.01) was observed between OD values from OF and DBS samples. The OF assay identified all DBS-confirmed positive and negative samples, resulting in 100% sensitivity and specificity. Conclusions Oral fluid is a valid alternative specimen for monitoring changes in antibodies to P. falciparum antigens. As OF collection is often more acceptable to communities, poses less of a biohazard than blood samples and can be performed by community volunteers, serological surveys using OF samples provide a strategy for monitoring population immunity in regions of declining malaria transmission. |
format |
Article in Journal/Newspaper |
author |
Mharakurwa Sungano Hamapumbu Harry Kobayashi Tamaki Chishimba Sandra Chidi Alexis P Thuma Philip E Moss William J |
author_facet |
Mharakurwa Sungano Hamapumbu Harry Kobayashi Tamaki Chishimba Sandra Chidi Alexis P Thuma Philip E Moss William J |
author_sort |
Mharakurwa Sungano |
title |
Validation of oral fluid samples to monitor serological changes to Plasmodium falciparum : An observational study in southern Zambia |
title_short |
Validation of oral fluid samples to monitor serological changes to Plasmodium falciparum : An observational study in southern Zambia |
title_full |
Validation of oral fluid samples to monitor serological changes to Plasmodium falciparum : An observational study in southern Zambia |
title_fullStr |
Validation of oral fluid samples to monitor serological changes to Plasmodium falciparum : An observational study in southern Zambia |
title_full_unstemmed |
Validation of oral fluid samples to monitor serological changes to Plasmodium falciparum : An observational study in southern Zambia |
title_sort |
validation of oral fluid samples to monitor serological changes to plasmodium falciparum : an observational study in southern zambia |
publisher |
BMC |
publishDate |
2011 |
url |
https://doi.org/10.1186/1475-2875-10-162 https://doaj.org/article/de4e815bb83f4af1957faac2437f8f28 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 10, Iss 1, p 162 (2011) |
op_relation |
http://www.malariajournal.com/content/10/1/162 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-10-162 1475-2875 https://doaj.org/article/de4e815bb83f4af1957faac2437f8f28 |
op_doi |
https://doi.org/10.1186/1475-2875-10-162 |
container_title |
Malaria Journal |
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10 |
container_issue |
1 |
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162 |
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1766341715850952704 |