Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1
Abstract Background Since 2008, the aquaculture production of Crassostrea gigas was heavily affected by mass mortalities associated to Ostreid herpesvirus 1 (OsHV-1) microvariants worldwide. Transcriptomic studies revealed the major antiviral pathways of the oyster immune response while other findin...
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ftdoajarticles:oai:doaj.org/article:de4dd03527dc465ea64eaa99e8a0f055 2023-05-15T15:58:22+02:00 Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1 Umberto Rosani Miriam Abbadi Timothy Green Chang-Ming Bai Edoardo Turolla Giuseppe Arcangeli K. Mathias Wegner Paola Venier 2020-09-01T00:00:00Z https://doi.org/10.1186/s12864-020-07026-7 https://doaj.org/article/de4dd03527dc465ea64eaa99e8a0f055 EN eng BMC http://link.springer.com/article/10.1186/s12864-020-07026-7 https://doaj.org/toc/1471-2164 doi:10.1186/s12864-020-07026-7 1471-2164 https://doaj.org/article/de4dd03527dc465ea64eaa99e8a0f055 BMC Genomics, Vol 21, Iss 1, Pp 1-18 (2020) Oyster miRNA OsHV-1 ADAR C. gigas miRNAome Biotechnology TP248.13-248.65 Genetics QH426-470 article 2020 ftdoajarticles https://doi.org/10.1186/s12864-020-07026-7 2022-12-31T15:55:50Z Abstract Background Since 2008, the aquaculture production of Crassostrea gigas was heavily affected by mass mortalities associated to Ostreid herpesvirus 1 (OsHV-1) microvariants worldwide. Transcriptomic studies revealed the major antiviral pathways of the oyster immune response while other findings suggested that also small non-coding RNAs (sncRNA) such as microRNAs might act as key regulators of the oyster response against OsHV-1. To explore the explicit connection between small non-coding and protein-coding transcripts, we performed paired whole transcriptome analysis of sncRNA and messenger RNA (mRNA) in six oysters selected for different intensities of OsHV-1 infection. Results The mRNA profiles of the naturally infected oysters were mostly governed by the transcriptional activity of OsHV-1, with several differentially expressed genes mapping to the interferon, toll, apoptosis, and pro-PO pathways. In contrast, miRNA profiles suggested more complex regulatory mechanisms, with 15 differentially expressed miRNAs (DE-miRNA) pointing to a possible modulation of the host response during OsHV-1 infection. We predicted 68 interactions between DE-miRNAs and oyster 3′-UTRs, but only few of them involved antiviral genes. The sncRNA reads assigned to OsHV-1 rather resembled mRNA degradation products, suggesting the absence of genuine viral miRNAs. Conclusions We provided data describing the miRNAome during OsHV-1 infection in C. gigas. This information can be used to understand the role of miRNAs in healthy and diseased oysters, to identify new targets for functional studies and, eventually to disentangle cause and effect relationships during viral infections in marine mollusks. Article in Journal/Newspaper Crassostrea gigas Directory of Open Access Journals: DOAJ Articles BMC Genomics 21 1 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Oyster miRNA OsHV-1 ADAR C. gigas miRNAome Biotechnology TP248.13-248.65 Genetics QH426-470 |
spellingShingle |
Oyster miRNA OsHV-1 ADAR C. gigas miRNAome Biotechnology TP248.13-248.65 Genetics QH426-470 Umberto Rosani Miriam Abbadi Timothy Green Chang-Ming Bai Edoardo Turolla Giuseppe Arcangeli K. Mathias Wegner Paola Venier Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1 |
topic_facet |
Oyster miRNA OsHV-1 ADAR C. gigas miRNAome Biotechnology TP248.13-248.65 Genetics QH426-470 |
description |
Abstract Background Since 2008, the aquaculture production of Crassostrea gigas was heavily affected by mass mortalities associated to Ostreid herpesvirus 1 (OsHV-1) microvariants worldwide. Transcriptomic studies revealed the major antiviral pathways of the oyster immune response while other findings suggested that also small non-coding RNAs (sncRNA) such as microRNAs might act as key regulators of the oyster response against OsHV-1. To explore the explicit connection between small non-coding and protein-coding transcripts, we performed paired whole transcriptome analysis of sncRNA and messenger RNA (mRNA) in six oysters selected for different intensities of OsHV-1 infection. Results The mRNA profiles of the naturally infected oysters were mostly governed by the transcriptional activity of OsHV-1, with several differentially expressed genes mapping to the interferon, toll, apoptosis, and pro-PO pathways. In contrast, miRNA profiles suggested more complex regulatory mechanisms, with 15 differentially expressed miRNAs (DE-miRNA) pointing to a possible modulation of the host response during OsHV-1 infection. We predicted 68 interactions between DE-miRNAs and oyster 3′-UTRs, but only few of them involved antiviral genes. The sncRNA reads assigned to OsHV-1 rather resembled mRNA degradation products, suggesting the absence of genuine viral miRNAs. Conclusions We provided data describing the miRNAome during OsHV-1 infection in C. gigas. This information can be used to understand the role of miRNAs in healthy and diseased oysters, to identify new targets for functional studies and, eventually to disentangle cause and effect relationships during viral infections in marine mollusks. |
format |
Article in Journal/Newspaper |
author |
Umberto Rosani Miriam Abbadi Timothy Green Chang-Ming Bai Edoardo Turolla Giuseppe Arcangeli K. Mathias Wegner Paola Venier |
author_facet |
Umberto Rosani Miriam Abbadi Timothy Green Chang-Ming Bai Edoardo Turolla Giuseppe Arcangeli K. Mathias Wegner Paola Venier |
author_sort |
Umberto Rosani |
title |
Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1 |
title_short |
Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1 |
title_full |
Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1 |
title_fullStr |
Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1 |
title_full_unstemmed |
Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1 |
title_sort |
parallel analysis of mirnas and mrnas suggests distinct regulatory networks in crassostrea gigas infected by ostreid herpesvirus 1 |
publisher |
BMC |
publishDate |
2020 |
url |
https://doi.org/10.1186/s12864-020-07026-7 https://doaj.org/article/de4dd03527dc465ea64eaa99e8a0f055 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
BMC Genomics, Vol 21, Iss 1, Pp 1-18 (2020) |
op_relation |
http://link.springer.com/article/10.1186/s12864-020-07026-7 https://doaj.org/toc/1471-2164 doi:10.1186/s12864-020-07026-7 1471-2164 https://doaj.org/article/de4dd03527dc465ea64eaa99e8a0f055 |
op_doi |
https://doi.org/10.1186/s12864-020-07026-7 |
container_title |
BMC Genomics |
container_volume |
21 |
container_issue |
1 |
_version_ |
1766394098587009024 |