The use of multiplex PCR for the detection of atypical pathogens in Egyptian children with CAP: a high rate of Bordetella pertussis in early infancy
Abstract Background Atypical pathogen infections played an important role in community-acquired pneumonia (CAP) in children. Pathogen-specific clinical symptoms are often lacking, and it is difficult to detect atypical pathogens by culture methods. The use of multiplex polymerase chain reaction (PCR...
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ftdoajarticles:oai:doaj.org/article:ddbe4af279a5467ba625a7e71c280895 2023-05-15T15:12:29+02:00 The use of multiplex PCR for the detection of atypical pathogens in Egyptian children with CAP: a high rate of Bordetella pertussis in early infancy Noussa R. El Basha Hala H. Shaaban Hassan A. El Atroush May M. Sherif Amani A. El Kholy 2019-01-01T00:00:00Z https://doi.org/10.1186/s42506-018-0003-4 https://doaj.org/article/ddbe4af279a5467ba625a7e71c280895 EN eng SpringerOpen http://link.springer.com/article/10.1186/s42506-018-0003-4 https://doaj.org/toc/2090-262X doi:10.1186/s42506-018-0003-4 2090-262X https://doaj.org/article/ddbe4af279a5467ba625a7e71c280895 Journal of the Egyptian Public Health Association, Vol 94, Iss 1, Pp 1-7 (2019) Community-acquired pneumonia Children Atypical pathogen Pertussis Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1186/s42506-018-0003-4 2022-12-31T09:53:30Z Abstract Background Atypical pathogen infections played an important role in community-acquired pneumonia (CAP) in children. Pathogen-specific clinical symptoms are often lacking, and it is difficult to detect atypical pathogens by culture methods. The use of multiplex polymerase chain reaction (PCR) methods enables testing for many pathogens simultaneously in a single analysis. Aim To determine the role of atypical pathogens in children hospitalized with CAP. Patients and methods This cross-sectional study was conducted throughout a 2-year period from August 2015 to September 2017. It included 400 Egyptian children hospitalized with clinical diagnosis of CAP at a tertiary hospital in Cairo, Egypt. Sputum samples were collected from lower respiratory tract of all enrolled patients by mucus trap catheter for identification of Bordetella pertussis, Mycoplasma pneumoniae, Chlamydia pneumoniae, and Legionella pneumophilia by using multiplex real-time PCR. Results Among the 400 CAP patients enrolled in this study, atypical pathogens were detected in 12/400 (3%) patients. Bordetella pertussis was detected in 2% of cases, and it was responsible for CAP in 8/104 (7.69%) infants in the age stratum ≤ 4 months; compared with pertussis-negative cases, pertussis-positive cases were younger and incompletely vaccinated (P values were 0.001 and 0.007, respectively). Mycoplasma pneumoniae was detected in 1% of cases, all were among the age stratum > 4 months ≤ 59 months in 4/272 (1.47%) children. Conclusion In early infancy, Bordetella pertussis causes a significant proportion of hospitalized CAP cases; all were ≤ 4 months old and incompletely vaccinated. This finding could suggest the role of maternal immunization in developing countries. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of the Egyptian Public Health Association 94 1 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Community-acquired pneumonia Children Atypical pathogen Pertussis Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Community-acquired pneumonia Children Atypical pathogen Pertussis Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Noussa R. El Basha Hala H. Shaaban Hassan A. El Atroush May M. Sherif Amani A. El Kholy The use of multiplex PCR for the detection of atypical pathogens in Egyptian children with CAP: a high rate of Bordetella pertussis in early infancy |
topic_facet |
Community-acquired pneumonia Children Atypical pathogen Pertussis Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Abstract Background Atypical pathogen infections played an important role in community-acquired pneumonia (CAP) in children. Pathogen-specific clinical symptoms are often lacking, and it is difficult to detect atypical pathogens by culture methods. The use of multiplex polymerase chain reaction (PCR) methods enables testing for many pathogens simultaneously in a single analysis. Aim To determine the role of atypical pathogens in children hospitalized with CAP. Patients and methods This cross-sectional study was conducted throughout a 2-year period from August 2015 to September 2017. It included 400 Egyptian children hospitalized with clinical diagnosis of CAP at a tertiary hospital in Cairo, Egypt. Sputum samples were collected from lower respiratory tract of all enrolled patients by mucus trap catheter for identification of Bordetella pertussis, Mycoplasma pneumoniae, Chlamydia pneumoniae, and Legionella pneumophilia by using multiplex real-time PCR. Results Among the 400 CAP patients enrolled in this study, atypical pathogens were detected in 12/400 (3%) patients. Bordetella pertussis was detected in 2% of cases, and it was responsible for CAP in 8/104 (7.69%) infants in the age stratum ≤ 4 months; compared with pertussis-negative cases, pertussis-positive cases were younger and incompletely vaccinated (P values were 0.001 and 0.007, respectively). Mycoplasma pneumoniae was detected in 1% of cases, all were among the age stratum > 4 months ≤ 59 months in 4/272 (1.47%) children. Conclusion In early infancy, Bordetella pertussis causes a significant proportion of hospitalized CAP cases; all were ≤ 4 months old and incompletely vaccinated. This finding could suggest the role of maternal immunization in developing countries. |
format |
Article in Journal/Newspaper |
author |
Noussa R. El Basha Hala H. Shaaban Hassan A. El Atroush May M. Sherif Amani A. El Kholy |
author_facet |
Noussa R. El Basha Hala H. Shaaban Hassan A. El Atroush May M. Sherif Amani A. El Kholy |
author_sort |
Noussa R. El Basha |
title |
The use of multiplex PCR for the detection of atypical pathogens in Egyptian children with CAP: a high rate of Bordetella pertussis in early infancy |
title_short |
The use of multiplex PCR for the detection of atypical pathogens in Egyptian children with CAP: a high rate of Bordetella pertussis in early infancy |
title_full |
The use of multiplex PCR for the detection of atypical pathogens in Egyptian children with CAP: a high rate of Bordetella pertussis in early infancy |
title_fullStr |
The use of multiplex PCR for the detection of atypical pathogens in Egyptian children with CAP: a high rate of Bordetella pertussis in early infancy |
title_full_unstemmed |
The use of multiplex PCR for the detection of atypical pathogens in Egyptian children with CAP: a high rate of Bordetella pertussis in early infancy |
title_sort |
use of multiplex pcr for the detection of atypical pathogens in egyptian children with cap: a high rate of bordetella pertussis in early infancy |
publisher |
SpringerOpen |
publishDate |
2019 |
url |
https://doi.org/10.1186/s42506-018-0003-4 https://doaj.org/article/ddbe4af279a5467ba625a7e71c280895 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of the Egyptian Public Health Association, Vol 94, Iss 1, Pp 1-7 (2019) |
op_relation |
http://link.springer.com/article/10.1186/s42506-018-0003-4 https://doaj.org/toc/2090-262X doi:10.1186/s42506-018-0003-4 2090-262X https://doaj.org/article/ddbe4af279a5467ba625a7e71c280895 |
op_doi |
https://doi.org/10.1186/s42506-018-0003-4 |
container_title |
Journal of the Egyptian Public Health Association |
container_volume |
94 |
container_issue |
1 |
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1766343151651389440 |