Performance evaluation of the highly sensitive histidine‐rich protein 2 rapid test for plasmodium falciparum malaria in North-West Tanzania
Abstract Background Precise detection of Plasmodium infections in community surveys is essential for effective malaria control. Microscopy and rapid diagnostic tests (RDTs) are the major techniques used to identify malaria infections in the field-based surveys. Although microscopy is still considere...
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ftdoajarticles:oai:doaj.org/article:dd7d8f6991ac4bca902386c275f7c058 2023-05-15T15:17:29+02:00 Performance evaluation of the highly sensitive histidine‐rich protein 2 rapid test for plasmodium falciparum malaria in North-West Tanzania Alphaxard Manjurano Justin J. Omolo Eric Lyimo Donald Miyaye Coleman Kishamawe Lucas E. Matemba Julius J. Massaga John Changalucha Paul E. Kazyoba 2021-01-01T00:00:00Z https://doi.org/10.1186/s12936-020-03568-z https://doaj.org/article/dd7d8f6991ac4bca902386c275f7c058 EN eng BMC https://doi.org/10.1186/s12936-020-03568-z https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03568-z 1475-2875 https://doaj.org/article/dd7d8f6991ac4bca902386c275f7c058 Malaria Journal, Vol 20, Iss 1, Pp 1-9 (2021) HSPf SD Bioline Pf Microscopy Sensitivity Specificity Plasmodium falciparum Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2021 ftdoajarticles https://doi.org/10.1186/s12936-020-03568-z 2022-12-31T06:27:42Z Abstract Background Precise detection of Plasmodium infections in community surveys is essential for effective malaria control. Microscopy and rapid diagnostic tests (RDTs) are the major techniques used to identify malaria infections in the field-based surveys. Although microscopy is still considered as the gold standard, RDTs are increasingly becoming versatile due to their rapid and adequate performance characteristics. Methods A malaria prevalence cross-sectional survey was carried out in north-western Tanzania in 2016, aimed at appraising the performance of high sensitivity Plasmodium falciparum (HSPf) tests compared to SD Bioline Pf and microscopy in detecting P. falciparum infections. A total of 397 individuals aged five years and above were tested for P. falciparum infections. The sensitivity, specificity, positive, and negative predictive values (PPV and NPV) of microscopy, Pf RDT and HSPf RDT was determined using PCR as the gold standard method. Results The prevalence of P. falciparum infections determined by microscopy, SD Bioline Pf, HSPf and PCR was 21.9, 27.7, 33.3 and 43.2%, respectively. The new HSPf RDT had significantly higher sensitivity (98.2%) and specificity (91.6%) compared to the routinely used SD Bioline Pf RDT(P < 0.001). The positive predictive value (PPV) was 81.8% and the negative predictive value (NPV) was 99.2% for the routinely used SD Bioline Pf RDT. Moreover, HSPf RDT had sensitivity of 69% and specificity of 76.8% compared to microscopy. The PPV was 45.5% and the NPV was 89.8% for microscopy. Furthermore, the analytical sensitivity test indicated that the newly developed HSPf RDT had lower detection limits compared to routinely used SD Bioline RDT. Conclusions HSPf RDT had better performance when compared to both microscopy and the currently used malaria RDTs. The false negativity could be associated with the low parasite density of the samples. False positivity may be related to the limitations of the expertise of microscopists or persistent antigenicity from previous ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 20 1 |
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HSPf SD Bioline Pf Microscopy Sensitivity Specificity Plasmodium falciparum Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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HSPf SD Bioline Pf Microscopy Sensitivity Specificity Plasmodium falciparum Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Alphaxard Manjurano Justin J. Omolo Eric Lyimo Donald Miyaye Coleman Kishamawe Lucas E. Matemba Julius J. Massaga John Changalucha Paul E. Kazyoba Performance evaluation of the highly sensitive histidine‐rich protein 2 rapid test for plasmodium falciparum malaria in North-West Tanzania |
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HSPf SD Bioline Pf Microscopy Sensitivity Specificity Plasmodium falciparum Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Precise detection of Plasmodium infections in community surveys is essential for effective malaria control. Microscopy and rapid diagnostic tests (RDTs) are the major techniques used to identify malaria infections in the field-based surveys. Although microscopy is still considered as the gold standard, RDTs are increasingly becoming versatile due to their rapid and adequate performance characteristics. Methods A malaria prevalence cross-sectional survey was carried out in north-western Tanzania in 2016, aimed at appraising the performance of high sensitivity Plasmodium falciparum (HSPf) tests compared to SD Bioline Pf and microscopy in detecting P. falciparum infections. A total of 397 individuals aged five years and above were tested for P. falciparum infections. The sensitivity, specificity, positive, and negative predictive values (PPV and NPV) of microscopy, Pf RDT and HSPf RDT was determined using PCR as the gold standard method. Results The prevalence of P. falciparum infections determined by microscopy, SD Bioline Pf, HSPf and PCR was 21.9, 27.7, 33.3 and 43.2%, respectively. The new HSPf RDT had significantly higher sensitivity (98.2%) and specificity (91.6%) compared to the routinely used SD Bioline Pf RDT(P < 0.001). The positive predictive value (PPV) was 81.8% and the negative predictive value (NPV) was 99.2% for the routinely used SD Bioline Pf RDT. Moreover, HSPf RDT had sensitivity of 69% and specificity of 76.8% compared to microscopy. The PPV was 45.5% and the NPV was 89.8% for microscopy. Furthermore, the analytical sensitivity test indicated that the newly developed HSPf RDT had lower detection limits compared to routinely used SD Bioline RDT. Conclusions HSPf RDT had better performance when compared to both microscopy and the currently used malaria RDTs. The false negativity could be associated with the low parasite density of the samples. False positivity may be related to the limitations of the expertise of microscopists or persistent antigenicity from previous ... |
format |
Article in Journal/Newspaper |
author |
Alphaxard Manjurano Justin J. Omolo Eric Lyimo Donald Miyaye Coleman Kishamawe Lucas E. Matemba Julius J. Massaga John Changalucha Paul E. Kazyoba |
author_facet |
Alphaxard Manjurano Justin J. Omolo Eric Lyimo Donald Miyaye Coleman Kishamawe Lucas E. Matemba Julius J. Massaga John Changalucha Paul E. Kazyoba |
author_sort |
Alphaxard Manjurano |
title |
Performance evaluation of the highly sensitive histidine‐rich protein 2 rapid test for plasmodium falciparum malaria in North-West Tanzania |
title_short |
Performance evaluation of the highly sensitive histidine‐rich protein 2 rapid test for plasmodium falciparum malaria in North-West Tanzania |
title_full |
Performance evaluation of the highly sensitive histidine‐rich protein 2 rapid test for plasmodium falciparum malaria in North-West Tanzania |
title_fullStr |
Performance evaluation of the highly sensitive histidine‐rich protein 2 rapid test for plasmodium falciparum malaria in North-West Tanzania |
title_full_unstemmed |
Performance evaluation of the highly sensitive histidine‐rich protein 2 rapid test for plasmodium falciparum malaria in North-West Tanzania |
title_sort |
performance evaluation of the highly sensitive histidine‐rich protein 2 rapid test for plasmodium falciparum malaria in north-west tanzania |
publisher |
BMC |
publishDate |
2021 |
url |
https://doi.org/10.1186/s12936-020-03568-z https://doaj.org/article/dd7d8f6991ac4bca902386c275f7c058 |
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Arctic |
geographic_facet |
Arctic |
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Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 20, Iss 1, Pp 1-9 (2021) |
op_relation |
https://doi.org/10.1186/s12936-020-03568-z https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03568-z 1475-2875 https://doaj.org/article/dd7d8f6991ac4bca902386c275f7c058 |
op_doi |
https://doi.org/10.1186/s12936-020-03568-z |
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Malaria Journal |
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20 |
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1766347725749616640 |