Microbiota identified from preserved Anopheles
Abstract Background Mosquito species from the Anopheles gambiae complex and the Anopheles funestus group are dominant African malaria vectors. Mosquito microbiota play vital roles in physiology and vector competence. Recent research has focused on investigating the mosquito microbiota, especially in...
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ftdoajarticles:oai:doaj.org/article:dce491edb9fe4cf98f546efedc8d3b39 2023-05-15T15:13:30+02:00 Microbiota identified from preserved Anopheles Bianca E Silva Zvifadzo Matsena Zingoni Lizette L. Koekemoer Yael L. Dahan-Moss 2021-05-01T00:00:00Z https://doi.org/10.1186/s12936-021-03754-7 https://doaj.org/article/dce491edb9fe4cf98f546efedc8d3b39 EN eng BMC https://doi.org/10.1186/s12936-021-03754-7 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-021-03754-7 1475-2875 https://doaj.org/article/dce491edb9fe4cf98f546efedc8d3b39 Malaria Journal, Vol 20, Iss 1, Pp 1-18 (2021) Anopheles arabiensis Anopheles funestus Culturomics Next-generation sequencing RNAlater ® Silica Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2021 ftdoajarticles https://doi.org/10.1186/s12936-021-03754-7 2022-12-31T06:11:18Z Abstract Background Mosquito species from the Anopheles gambiae complex and the Anopheles funestus group are dominant African malaria vectors. Mosquito microbiota play vital roles in physiology and vector competence. Recent research has focused on investigating the mosquito microbiota, especially in wild populations. Wild mosquitoes are preserved and transported to a laboratory for analyses. Thus far, microbial characterization post-preservation has been investigated in only Aedes vexans and Culex pipiens. Investigating the efficacy of cost-effective preservatives has also been limited to AllProtect reagent, ethanol and nucleic acid preservation buffer. This study characterized the microbiota of African Anopheles vectors: Anopheles arabiensis (member of the An. gambiae complex) and An. funestus (member of the An. funestus group), preserved on silica desiccant and RNAlater ® solution. Methods Microbial composition and diversity were characterized using culture-dependent (midgut dissections, culturomics, MALDI-TOF MS) and culture-independent techniques (abdominal dissections, DNA extraction, next-generation sequencing) from laboratory (colonized) and field-collected mosquitoes. Colonized mosquitoes were either fresh (non-preserved) or preserved for 4 and 12 weeks on silica or in RNAlater ®. Microbiota were also characterized from field-collected An. arabiensis preserved on silica for 8, 12 and 16 weeks. Results Elizabethkingia anophelis and Serratia oryzae were common between both vector species, while Enterobacter cloacae and Staphylococcus epidermidis were specific to females and males, respectively. Microbial diversity was not influenced by sex, condition (fresh or preserved), preservative, or preservation time-period; however, the type of bacterial identification technique affected all microbial diversity indices. Conclusions This study broadly characterized the microbiota of An. arabiensis and An. funestus. Silica- and RNAlater ®-preservation were appropriate when paired with culture-dependent and ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 20 1 |
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topic |
Anopheles arabiensis Anopheles funestus Culturomics Next-generation sequencing RNAlater ® Silica Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Anopheles arabiensis Anopheles funestus Culturomics Next-generation sequencing RNAlater ® Silica Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Bianca E Silva Zvifadzo Matsena Zingoni Lizette L. Koekemoer Yael L. Dahan-Moss Microbiota identified from preserved Anopheles |
topic_facet |
Anopheles arabiensis Anopheles funestus Culturomics Next-generation sequencing RNAlater ® Silica Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Mosquito species from the Anopheles gambiae complex and the Anopheles funestus group are dominant African malaria vectors. Mosquito microbiota play vital roles in physiology and vector competence. Recent research has focused on investigating the mosquito microbiota, especially in wild populations. Wild mosquitoes are preserved and transported to a laboratory for analyses. Thus far, microbial characterization post-preservation has been investigated in only Aedes vexans and Culex pipiens. Investigating the efficacy of cost-effective preservatives has also been limited to AllProtect reagent, ethanol and nucleic acid preservation buffer. This study characterized the microbiota of African Anopheles vectors: Anopheles arabiensis (member of the An. gambiae complex) and An. funestus (member of the An. funestus group), preserved on silica desiccant and RNAlater ® solution. Methods Microbial composition and diversity were characterized using culture-dependent (midgut dissections, culturomics, MALDI-TOF MS) and culture-independent techniques (abdominal dissections, DNA extraction, next-generation sequencing) from laboratory (colonized) and field-collected mosquitoes. Colonized mosquitoes were either fresh (non-preserved) or preserved for 4 and 12 weeks on silica or in RNAlater ®. Microbiota were also characterized from field-collected An. arabiensis preserved on silica for 8, 12 and 16 weeks. Results Elizabethkingia anophelis and Serratia oryzae were common between both vector species, while Enterobacter cloacae and Staphylococcus epidermidis were specific to females and males, respectively. Microbial diversity was not influenced by sex, condition (fresh or preserved), preservative, or preservation time-period; however, the type of bacterial identification technique affected all microbial diversity indices. Conclusions This study broadly characterized the microbiota of An. arabiensis and An. funestus. Silica- and RNAlater ®-preservation were appropriate when paired with culture-dependent and ... |
format |
Article in Journal/Newspaper |
author |
Bianca E Silva Zvifadzo Matsena Zingoni Lizette L. Koekemoer Yael L. Dahan-Moss |
author_facet |
Bianca E Silva Zvifadzo Matsena Zingoni Lizette L. Koekemoer Yael L. Dahan-Moss |
author_sort |
Bianca E Silva |
title |
Microbiota identified from preserved Anopheles |
title_short |
Microbiota identified from preserved Anopheles |
title_full |
Microbiota identified from preserved Anopheles |
title_fullStr |
Microbiota identified from preserved Anopheles |
title_full_unstemmed |
Microbiota identified from preserved Anopheles |
title_sort |
microbiota identified from preserved anopheles |
publisher |
BMC |
publishDate |
2021 |
url |
https://doi.org/10.1186/s12936-021-03754-7 https://doaj.org/article/dce491edb9fe4cf98f546efedc8d3b39 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 20, Iss 1, Pp 1-18 (2021) |
op_relation |
https://doi.org/10.1186/s12936-021-03754-7 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-021-03754-7 1475-2875 https://doaj.org/article/dce491edb9fe4cf98f546efedc8d3b39 |
op_doi |
https://doi.org/10.1186/s12936-021-03754-7 |
container_title |
Malaria Journal |
container_volume |
20 |
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1 |
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1766344056002052096 |