Comparative analysis of the venom proteome of four important Malaysian snake species
BackgroundNaja kaouthia, Ophiophagus hannah, Bungarus fasciatusandCalloselasma rhodostomaare four venomous snakes indigenous to Malaysia. In the present study, their proteomic profile by two-dimensional gel electrophoresis (2-DE) have been separated and compared.ResultsThe 2-DE of venoms of the four...
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ftdoajarticles:oai:doaj.org/article:dcdc1e660deb421aafd5e8633a509621 2023-05-15T15:04:10+02:00 Comparative analysis of the venom proteome of four important Malaysian snake species Jaya Vejayan Too Lay Khoon Halijah Ibrahim 2014-03-01T00:00:00Z https://doi.org/10.1186/1678-9199-20-6 https://doaj.org/article/dcdc1e660deb421aafd5e8633a509621 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200317&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/1678-9199-20-6 https://doaj.org/article/dcdc1e660deb421aafd5e8633a509621 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 20, Iss 0 (2014) Mass spectrometry Snake venoms Proteomics Two-dimensional gel electrophoresis Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2014 ftdoajarticles https://doi.org/10.1186/1678-9199-20-6 2022-12-31T03:37:20Z BackgroundNaja kaouthia, Ophiophagus hannah, Bungarus fasciatusandCalloselasma rhodostomaare four venomous snakes indigenous to Malaysia. In the present study, their proteomic profile by two-dimensional gel electrophoresis (2-DE) have been separated and compared.ResultsThe 2-DE of venoms of the four species snake demonstrated complexity and obvious interspecies differences in proteome profiles. A total of 63 proteins were identified in the four species: C. rhodostoma -26, N. kaouthia -16, O. hannah -15 and B. fasciatus -6.ConclusionsDespite the identifications of major proteins in the four snake species, a large number of protein spots from the 2-DE were unidentified even though the spots displayed high-quality MALDI-TOF-MS spectra. Those identified included phospholipase A2 proteins in all four venoms, long neurotoxins in both cobra species and the C. rhodostomavenom found with the most varied types of peptidases, i.e. metalloproteinase kistomin, halystase and L-amino acid oxidase. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Hannah ENVELOPE(-60.613,-60.613,-62.654,-62.654) Journal of Venomous Animals and Toxins including Tropical Diseases 20 1 6 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Mass spectrometry Snake venoms Proteomics Two-dimensional gel electrophoresis Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
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Mass spectrometry Snake venoms Proteomics Two-dimensional gel electrophoresis Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Jaya Vejayan Too Lay Khoon Halijah Ibrahim Comparative analysis of the venom proteome of four important Malaysian snake species |
topic_facet |
Mass spectrometry Snake venoms Proteomics Two-dimensional gel electrophoresis Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
BackgroundNaja kaouthia, Ophiophagus hannah, Bungarus fasciatusandCalloselasma rhodostomaare four venomous snakes indigenous to Malaysia. In the present study, their proteomic profile by two-dimensional gel electrophoresis (2-DE) have been separated and compared.ResultsThe 2-DE of venoms of the four species snake demonstrated complexity and obvious interspecies differences in proteome profiles. A total of 63 proteins were identified in the four species: C. rhodostoma -26, N. kaouthia -16, O. hannah -15 and B. fasciatus -6.ConclusionsDespite the identifications of major proteins in the four snake species, a large number of protein spots from the 2-DE were unidentified even though the spots displayed high-quality MALDI-TOF-MS spectra. Those identified included phospholipase A2 proteins in all four venoms, long neurotoxins in both cobra species and the C. rhodostomavenom found with the most varied types of peptidases, i.e. metalloproteinase kistomin, halystase and L-amino acid oxidase. |
format |
Article in Journal/Newspaper |
author |
Jaya Vejayan Too Lay Khoon Halijah Ibrahim |
author_facet |
Jaya Vejayan Too Lay Khoon Halijah Ibrahim |
author_sort |
Jaya Vejayan |
title |
Comparative analysis of the venom proteome of four important Malaysian snake species |
title_short |
Comparative analysis of the venom proteome of four important Malaysian snake species |
title_full |
Comparative analysis of the venom proteome of four important Malaysian snake species |
title_fullStr |
Comparative analysis of the venom proteome of four important Malaysian snake species |
title_full_unstemmed |
Comparative analysis of the venom proteome of four important Malaysian snake species |
title_sort |
comparative analysis of the venom proteome of four important malaysian snake species |
publisher |
SciELO |
publishDate |
2014 |
url |
https://doi.org/10.1186/1678-9199-20-6 https://doaj.org/article/dcdc1e660deb421aafd5e8633a509621 |
long_lat |
ENVELOPE(-60.613,-60.613,-62.654,-62.654) |
geographic |
Arctic Hannah |
geographic_facet |
Arctic Hannah |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 20, Iss 0 (2014) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200317&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/1678-9199-20-6 https://doaj.org/article/dcdc1e660deb421aafd5e8633a509621 |
op_doi |
https://doi.org/10.1186/1678-9199-20-6 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
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20 |
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1 |
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6 |
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1766335979533107200 |