Genetic diversity of Plasmodium vivax in Kolkata, India
Abstract Background Plasmodium vivax malaria accounts for approximately 60% of malaria cases in Kolkata, India. There has been limited information on the genotypic polymorphism of P. vivax in this malaria endemic area. Three highly polymorphic and single copy genes were selected for a study of genet...
Published in: | Malaria Journal |
---|---|
Main Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2006
|
Subjects: | |
Online Access: | https://doi.org/10.1186/1475-2875-5-71 https://doaj.org/article/dc7c7fcd3d874ea29ce78f920203c55f |
id |
ftdoajarticles:oai:doaj.org/article:dc7c7fcd3d874ea29ce78f920203c55f |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:dc7c7fcd3d874ea29ce78f920203c55f 2023-05-15T15:08:42+02:00 Genetic diversity of Plasmodium vivax in Kolkata, India Day Nick PJ Addy Manjulika Maji Ardhendu Tonomsing Naowarat Nontprasert Apichart Chotivanich Kesinee Nandy Amitabha Imwong Mallika Kim Jung-Ryong White Nicholas J Pukrittayakamee Sasithon 2006-08-01T00:00:00Z https://doi.org/10.1186/1475-2875-5-71 https://doaj.org/article/dc7c7fcd3d874ea29ce78f920203c55f EN eng BMC http://www.malariajournal.com/content/5/1/71 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-5-71 1475-2875 https://doaj.org/article/dc7c7fcd3d874ea29ce78f920203c55f Malaria Journal, Vol 5, Iss 1, p 71 (2006) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2006 ftdoajarticles https://doi.org/10.1186/1475-2875-5-71 2022-12-31T02:28:16Z Abstract Background Plasmodium vivax malaria accounts for approximately 60% of malaria cases in Kolkata, India. There has been limited information on the genotypic polymorphism of P. vivax in this malaria endemic area. Three highly polymorphic and single copy genes were selected for a study of genetic diversity in Kolkata strains. Methods Blood from 151 patients with P. vivax infection diagnosed in Kolkata between April 2003 and September 2004 was genotyped at three polymorphic loci: the P. vivax circumsporozoite protein ( pvcs ), the merozoite surface protein 1 ( pvmsp 1) and the merozoite surface protein 3-alpha ( pvmsp 3-alpha). Results Analysis of these three genetic markers revealed that P. vivax populations in Kolkata are highly diverse. A large number of distinguishable alleles were found from three genetic markers: 11 for pvcs , 35 for pvmsp 1 and 37 for pvmsp 3-alpha. These were, in general, randomly distributed amongst the isolates. Among the 151 isolates, 142 unique genotypes were detected the commonest genotype at a frequency of less than 2% (3/151). The overall rate of mixed genotype infections was 10.6%. Conclusion These results indicate that the P. vivax parasite population is highly diverse in Kolkata, despite the low level of transmission. The genotyping protocols used in this study may be useful for differentiating re-infection from relapse and recrudescence in studies assessing of malarial drug efficacy in vivax malaria. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 5 1 71 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Day Nick PJ Addy Manjulika Maji Ardhendu Tonomsing Naowarat Nontprasert Apichart Chotivanich Kesinee Nandy Amitabha Imwong Mallika Kim Jung-Ryong White Nicholas J Pukrittayakamee Sasithon Genetic diversity of Plasmodium vivax in Kolkata, India |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Plasmodium vivax malaria accounts for approximately 60% of malaria cases in Kolkata, India. There has been limited information on the genotypic polymorphism of P. vivax in this malaria endemic area. Three highly polymorphic and single copy genes were selected for a study of genetic diversity in Kolkata strains. Methods Blood from 151 patients with P. vivax infection diagnosed in Kolkata between April 2003 and September 2004 was genotyped at three polymorphic loci: the P. vivax circumsporozoite protein ( pvcs ), the merozoite surface protein 1 ( pvmsp 1) and the merozoite surface protein 3-alpha ( pvmsp 3-alpha). Results Analysis of these three genetic markers revealed that P. vivax populations in Kolkata are highly diverse. A large number of distinguishable alleles were found from three genetic markers: 11 for pvcs , 35 for pvmsp 1 and 37 for pvmsp 3-alpha. These were, in general, randomly distributed amongst the isolates. Among the 151 isolates, 142 unique genotypes were detected the commonest genotype at a frequency of less than 2% (3/151). The overall rate of mixed genotype infections was 10.6%. Conclusion These results indicate that the P. vivax parasite population is highly diverse in Kolkata, despite the low level of transmission. The genotyping protocols used in this study may be useful for differentiating re-infection from relapse and recrudescence in studies assessing of malarial drug efficacy in vivax malaria. |
format |
Article in Journal/Newspaper |
author |
Day Nick PJ Addy Manjulika Maji Ardhendu Tonomsing Naowarat Nontprasert Apichart Chotivanich Kesinee Nandy Amitabha Imwong Mallika Kim Jung-Ryong White Nicholas J Pukrittayakamee Sasithon |
author_facet |
Day Nick PJ Addy Manjulika Maji Ardhendu Tonomsing Naowarat Nontprasert Apichart Chotivanich Kesinee Nandy Amitabha Imwong Mallika Kim Jung-Ryong White Nicholas J Pukrittayakamee Sasithon |
author_sort |
Day Nick PJ |
title |
Genetic diversity of Plasmodium vivax in Kolkata, India |
title_short |
Genetic diversity of Plasmodium vivax in Kolkata, India |
title_full |
Genetic diversity of Plasmodium vivax in Kolkata, India |
title_fullStr |
Genetic diversity of Plasmodium vivax in Kolkata, India |
title_full_unstemmed |
Genetic diversity of Plasmodium vivax in Kolkata, India |
title_sort |
genetic diversity of plasmodium vivax in kolkata, india |
publisher |
BMC |
publishDate |
2006 |
url |
https://doi.org/10.1186/1475-2875-5-71 https://doaj.org/article/dc7c7fcd3d874ea29ce78f920203c55f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 5, Iss 1, p 71 (2006) |
op_relation |
http://www.malariajournal.com/content/5/1/71 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-5-71 1475-2875 https://doaj.org/article/dc7c7fcd3d874ea29ce78f920203c55f |
op_doi |
https://doi.org/10.1186/1475-2875-5-71 |
container_title |
Malaria Journal |
container_volume |
5 |
container_issue |
1 |
container_start_page |
71 |
_version_ |
1766340015549317120 |