Characterization of a Trichinella spiralis putative serine protease. Study of its potential as sero-diagnostic tool.
Trichinellosis is a serious zoonositc parasitosis worldwide. Because its clinical manifestations aren't specific, the diagnosis of trichinellosis is not easy to be made. Trichinella spiralis muscle larva (ML) excretory-secretory (ES) antigens are the most widely applied diagnostic antigens for...
Published in: | PLOS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2018
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0006485 https://doaj.org/article/dc417efdfc50455683b131603f18ed32 |
id |
ftdoajarticles:oai:doaj.org/article:dc417efdfc50455683b131603f18ed32 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:dc417efdfc50455683b131603f18ed32 2023-05-15T15:17:12+02:00 Characterization of a Trichinella spiralis putative serine protease. Study of its potential as sero-diagnostic tool. Ge Ge Sun Yan Yan Song Peng Jiang Hua Na Ren Shu Wei Yan Yue Han Ruo Dan Liu Xi Zhang Zhong Quan Wang Jing Cui 2018-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006485 https://doaj.org/article/dc417efdfc50455683b131603f18ed32 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5967804?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006485 https://doaj.org/article/dc417efdfc50455683b131603f18ed32 PLoS Neglected Tropical Diseases, Vol 12, Iss 5, p e0006485 (2018) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2018 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006485 2022-12-31T01:32:52Z Trichinellosis is a serious zoonositc parasitosis worldwide. Because its clinical manifestations aren't specific, the diagnosis of trichinellosis is not easy to be made. Trichinella spiralis muscle larva (ML) excretory-secretory (ES) antigens are the most widely applied diagnostic antigens for human trichinellosis, but the major drawback of the ES antigens for assaying anti-Trichinella antibodies is the false negative in the early Trichinella infection period. The aim of this study was to characterize the T. spiralis putative serine protease (TsSP) and to investigate its potential use for diagnosis of trichinellosis.The full-length TsSP sequence was cloned and expressed, and recombinant TsSP (rTsSP) was purified by Ni-NTA-Sefinose Column. On Western blotting analysis the rTsSP was recognized by T. spiralis-infected mouse serum, and the natural TsSP was identified in T. spiralis ML crude and ES antigens by using anti-rTsSP serum. Expression of TsSP was detected at various T. spiralis developmental stages (newborn larvae, muscle larvae, intestinal infective larvae and adult worms). Immunolocalization identified the TsSP principally in cuticles and stichosomes of the nematode. The sensitivity of rTsSP-ELISA and ES-ELISA was 98.11% (52/53) and 88.68% (47/53) respectively (P > 0.05) when the sera from trichinellosis patients were examined. However, while twenty-one serum samples of trichinellosis patients' sera at 19 days post-infection (dpi) were tested, the sensitivity (95.24%) of rTsSP-ELISA was distinctly higher than 71.43% of ES-ELISA (P < 0.05). The specificity (99.53%) of rTsSP-ELISA was remarkably higher than 91.98% of ES-ELISA (P < 0.01). Only one out of 20 serum samples of cysticercosis patients cross-reacted with the rTsSP. Specific anti-Trichinella IgG in infected mice was first detected by rTsSP-ELISA as soon as 7 dpi and antibody positive rate reached 100% on 10 dpi, whereas the ES-ELISA did not permit detection of 100% of infected mice before 16 dpi.The rTsSP is a potential early diagnostic ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 12 5 e0006485 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Ge Ge Sun Yan Yan Song Peng Jiang Hua Na Ren Shu Wei Yan Yue Han Ruo Dan Liu Xi Zhang Zhong Quan Wang Jing Cui Characterization of a Trichinella spiralis putative serine protease. Study of its potential as sero-diagnostic tool. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Trichinellosis is a serious zoonositc parasitosis worldwide. Because its clinical manifestations aren't specific, the diagnosis of trichinellosis is not easy to be made. Trichinella spiralis muscle larva (ML) excretory-secretory (ES) antigens are the most widely applied diagnostic antigens for human trichinellosis, but the major drawback of the ES antigens for assaying anti-Trichinella antibodies is the false negative in the early Trichinella infection period. The aim of this study was to characterize the T. spiralis putative serine protease (TsSP) and to investigate its potential use for diagnosis of trichinellosis.The full-length TsSP sequence was cloned and expressed, and recombinant TsSP (rTsSP) was purified by Ni-NTA-Sefinose Column. On Western blotting analysis the rTsSP was recognized by T. spiralis-infected mouse serum, and the natural TsSP was identified in T. spiralis ML crude and ES antigens by using anti-rTsSP serum. Expression of TsSP was detected at various T. spiralis developmental stages (newborn larvae, muscle larvae, intestinal infective larvae and adult worms). Immunolocalization identified the TsSP principally in cuticles and stichosomes of the nematode. The sensitivity of rTsSP-ELISA and ES-ELISA was 98.11% (52/53) and 88.68% (47/53) respectively (P > 0.05) when the sera from trichinellosis patients were examined. However, while twenty-one serum samples of trichinellosis patients' sera at 19 days post-infection (dpi) were tested, the sensitivity (95.24%) of rTsSP-ELISA was distinctly higher than 71.43% of ES-ELISA (P < 0.05). The specificity (99.53%) of rTsSP-ELISA was remarkably higher than 91.98% of ES-ELISA (P < 0.01). Only one out of 20 serum samples of cysticercosis patients cross-reacted with the rTsSP. Specific anti-Trichinella IgG in infected mice was first detected by rTsSP-ELISA as soon as 7 dpi and antibody positive rate reached 100% on 10 dpi, whereas the ES-ELISA did not permit detection of 100% of infected mice before 16 dpi.The rTsSP is a potential early diagnostic ... |
format |
Article in Journal/Newspaper |
author |
Ge Ge Sun Yan Yan Song Peng Jiang Hua Na Ren Shu Wei Yan Yue Han Ruo Dan Liu Xi Zhang Zhong Quan Wang Jing Cui |
author_facet |
Ge Ge Sun Yan Yan Song Peng Jiang Hua Na Ren Shu Wei Yan Yue Han Ruo Dan Liu Xi Zhang Zhong Quan Wang Jing Cui |
author_sort |
Ge Ge Sun |
title |
Characterization of a Trichinella spiralis putative serine protease. Study of its potential as sero-diagnostic tool. |
title_short |
Characterization of a Trichinella spiralis putative serine protease. Study of its potential as sero-diagnostic tool. |
title_full |
Characterization of a Trichinella spiralis putative serine protease. Study of its potential as sero-diagnostic tool. |
title_fullStr |
Characterization of a Trichinella spiralis putative serine protease. Study of its potential as sero-diagnostic tool. |
title_full_unstemmed |
Characterization of a Trichinella spiralis putative serine protease. Study of its potential as sero-diagnostic tool. |
title_sort |
characterization of a trichinella spiralis putative serine protease. study of its potential as sero-diagnostic tool. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pntd.0006485 https://doaj.org/article/dc417efdfc50455683b131603f18ed32 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 12, Iss 5, p e0006485 (2018) |
op_relation |
http://europepmc.org/articles/PMC5967804?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006485 https://doaj.org/article/dc417efdfc50455683b131603f18ed32 |
op_doi |
https://doi.org/10.1371/journal.pntd.0006485 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
12 |
container_issue |
5 |
container_start_page |
e0006485 |
_version_ |
1766347464896413696 |