MicroRNA Transcriptome Profiling in Heart of Trypanosoma cruzi-Infected Mice: Parasitological and Cardiological Outcomes.
Chagas disease is caused by the parasite Trypanosoma cruzi, and it begins with a short acute phase characterized by high parasitemia followed by a life-long chronic phase with scarce parasitism. Cardiac involvement is the most prominent manifestation, as 30% of infected subjects will develop abnorma...
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ftdoajarticles:oai:doaj.org/article:dc2fa22fe36243688c361143187f0173 2023-05-15T15:13:42+02:00 MicroRNA Transcriptome Profiling in Heart of Trypanosoma cruzi-Infected Mice: Parasitological and Cardiological Outcomes. Isabela Cunha Navarro Frederico Moraes Ferreira Helder I Nakaya Monique Andrade Baron Gláucia Vilar-Pereira Isabela Resende Pereira Ana Maria Gonçalves Silva Juliana Monte Real Thales De Brito Christophe Chevillard Joseli Lannes-Vieira Jorge Kalil Edecio Cunha-Neto Ludmila Rodrigues Pinto Ferreira 2015-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003828 https://doaj.org/article/dc2fa22fe36243688c361143187f0173 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4473529?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003828 https://doaj.org/article/dc2fa22fe36243688c361143187f0173 PLoS Neglected Tropical Diseases, Vol 9, Iss 6, p e0003828 (2015) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2015 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003828 2022-12-30T22:09:21Z Chagas disease is caused by the parasite Trypanosoma cruzi, and it begins with a short acute phase characterized by high parasitemia followed by a life-long chronic phase with scarce parasitism. Cardiac involvement is the most prominent manifestation, as 30% of infected subjects will develop abnormal ventricular repolarization with myocarditis, fibrosis and cardiomyocyte hypertrophy by undefined mechanisms. Nevertheless, follow-up studies in chagasic patients, as well as studies with murine models, suggest that the intensity of clinical symptoms and pathophysiological events that occur during the acute phase of disease are associated with the severity of cardiac disease observed during the chronic phase. In the present study we investigated the role of microRNAs (miRNAs) in the disease progression in response to T. cruzi infection, as alterations in miRNA levels are known to be associated with many cardiovascular disorders. We screened 641 rodent miRNAs in heart samples of mice during an acute infection with the Colombiana T.cruzi strain and identified multiple miRNAs significantly altered upon infection. Seventeen miRNAs were found significantly deregulated in all three analyzed time points post infection. Among these, six miRNAs had their expression correlated with clinical parameters relevant to the disease, such as parasitemia and maximal heart rate-corrected QT (QTc) interval. Computational analyses identified that the gene targets for these six miRNAs were involved in networks and signaling pathways related to increased ventricular depolarization and repolarization times, important factors for QTc interval prolongation. The data presented here will guide further studies about the contribution of microRNAs to Chagas heart disease pathogenesis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 9 6 e0003828 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Isabela Cunha Navarro Frederico Moraes Ferreira Helder I Nakaya Monique Andrade Baron Gláucia Vilar-Pereira Isabela Resende Pereira Ana Maria Gonçalves Silva Juliana Monte Real Thales De Brito Christophe Chevillard Joseli Lannes-Vieira Jorge Kalil Edecio Cunha-Neto Ludmila Rodrigues Pinto Ferreira MicroRNA Transcriptome Profiling in Heart of Trypanosoma cruzi-Infected Mice: Parasitological and Cardiological Outcomes. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Chagas disease is caused by the parasite Trypanosoma cruzi, and it begins with a short acute phase characterized by high parasitemia followed by a life-long chronic phase with scarce parasitism. Cardiac involvement is the most prominent manifestation, as 30% of infected subjects will develop abnormal ventricular repolarization with myocarditis, fibrosis and cardiomyocyte hypertrophy by undefined mechanisms. Nevertheless, follow-up studies in chagasic patients, as well as studies with murine models, suggest that the intensity of clinical symptoms and pathophysiological events that occur during the acute phase of disease are associated with the severity of cardiac disease observed during the chronic phase. In the present study we investigated the role of microRNAs (miRNAs) in the disease progression in response to T. cruzi infection, as alterations in miRNA levels are known to be associated with many cardiovascular disorders. We screened 641 rodent miRNAs in heart samples of mice during an acute infection with the Colombiana T.cruzi strain and identified multiple miRNAs significantly altered upon infection. Seventeen miRNAs were found significantly deregulated in all three analyzed time points post infection. Among these, six miRNAs had their expression correlated with clinical parameters relevant to the disease, such as parasitemia and maximal heart rate-corrected QT (QTc) interval. Computational analyses identified that the gene targets for these six miRNAs were involved in networks and signaling pathways related to increased ventricular depolarization and repolarization times, important factors for QTc interval prolongation. The data presented here will guide further studies about the contribution of microRNAs to Chagas heart disease pathogenesis. |
format |
Article in Journal/Newspaper |
author |
Isabela Cunha Navarro Frederico Moraes Ferreira Helder I Nakaya Monique Andrade Baron Gláucia Vilar-Pereira Isabela Resende Pereira Ana Maria Gonçalves Silva Juliana Monte Real Thales De Brito Christophe Chevillard Joseli Lannes-Vieira Jorge Kalil Edecio Cunha-Neto Ludmila Rodrigues Pinto Ferreira |
author_facet |
Isabela Cunha Navarro Frederico Moraes Ferreira Helder I Nakaya Monique Andrade Baron Gláucia Vilar-Pereira Isabela Resende Pereira Ana Maria Gonçalves Silva Juliana Monte Real Thales De Brito Christophe Chevillard Joseli Lannes-Vieira Jorge Kalil Edecio Cunha-Neto Ludmila Rodrigues Pinto Ferreira |
author_sort |
Isabela Cunha Navarro |
title |
MicroRNA Transcriptome Profiling in Heart of Trypanosoma cruzi-Infected Mice: Parasitological and Cardiological Outcomes. |
title_short |
MicroRNA Transcriptome Profiling in Heart of Trypanosoma cruzi-Infected Mice: Parasitological and Cardiological Outcomes. |
title_full |
MicroRNA Transcriptome Profiling in Heart of Trypanosoma cruzi-Infected Mice: Parasitological and Cardiological Outcomes. |
title_fullStr |
MicroRNA Transcriptome Profiling in Heart of Trypanosoma cruzi-Infected Mice: Parasitological and Cardiological Outcomes. |
title_full_unstemmed |
MicroRNA Transcriptome Profiling in Heart of Trypanosoma cruzi-Infected Mice: Parasitological and Cardiological Outcomes. |
title_sort |
microrna transcriptome profiling in heart of trypanosoma cruzi-infected mice: parasitological and cardiological outcomes. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pntd.0003828 https://doaj.org/article/dc2fa22fe36243688c361143187f0173 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 9, Iss 6, p e0003828 (2015) |
op_relation |
http://europepmc.org/articles/PMC4473529?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003828 https://doaj.org/article/dc2fa22fe36243688c361143187f0173 |
op_doi |
https://doi.org/10.1371/journal.pntd.0003828 |
container_title |
PLOS Neglected Tropical Diseases |
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9 |
container_issue |
6 |
container_start_page |
e0003828 |
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1766344219609268224 |