Laboratory Replication of Ostreid Herpes Virus (OsHV-1) Using Pacific Oyster Tissue Explants
Pacific oysters ( Crassostrea or Magallana gigas ) are one of the most economically important aquaculture species globally. Over the past two decades, ostreid herpesvirus (OsHV-1) has become a major pathogen of cultured Pacific oysters, resulting in widespread mortality with a global distribution. E...
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ftdoajarticles:oai:doaj.org/article:dbfc63dd81c94a49aaad1cfb25f95d39 2024-09-15T18:29:05+00:00 Laboratory Replication of Ostreid Herpes Virus (OsHV-1) Using Pacific Oyster Tissue Explants Robert W. A. Potts Tim Regan Stuart Ross Kelly Bateman Chantelle Hooper Richard Paley Ross D. Houston Tim P. Bean 2024-08-01T00:00:00Z https://doi.org/10.3390/v16081343 https://doaj.org/article/dbfc63dd81c94a49aaad1cfb25f95d39 EN eng MDPI AG https://www.mdpi.com/1999-4915/16/8/1343 https://doaj.org/toc/1999-4915 doi:10.3390/v16081343 1999-4915 https://doaj.org/article/dbfc63dd81c94a49aaad1cfb25f95d39 Viruses, Vol 16, Iss 8, p 1343 (2024) oyster tissue culture explants Microbiology QR1-502 article 2024 ftdoajarticles https://doi.org/10.3390/v16081343 2024-09-02T15:34:37Z Pacific oysters ( Crassostrea or Magallana gigas ) are one of the most economically important aquaculture species globally. Over the past two decades, ostreid herpesvirus (OsHV-1) has become a major pathogen of cultured Pacific oysters, resulting in widespread mortality with a global distribution. Experimental use of OsHV-1 is challenging for many reasons, including both complexity of host–pathogen dynamics and a lack of functioning model systems. The goal of this study was to improve the tools available for working with OsHV-1 in both whole animals and in tissue explants established from oysters maintained in controlled laboratory conditions. Tissue explants were taken from oysters originating from two different sources that have different levels of mortality in experimental OsHV-1 infections and were exposed to OsHV-1. A whole-animal infection experiment was run concurrently as a comparison. Quantitative PCR and electron microscopy were used to confirm that the explants were capable of replicating OsHV-1. Furthermore, the quantitative PCR results suggest that the source of the oysters was significant in determining the outcome of infection in the explants, supporting the validity of the explant model for OsHV-1 infection. This tissue explant approach for studying OsHV-1 allows for the control of confounding factors in the disease outcome that is not possible in whole-animal experiments, providing a new tool for the study of OsHV-1 in Pacific oysters. Article in Journal/Newspaper Pacific oyster Directory of Open Access Journals: DOAJ Articles Viruses 16 8 1343 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
oyster tissue culture explants Microbiology QR1-502 |
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oyster tissue culture explants Microbiology QR1-502 Robert W. A. Potts Tim Regan Stuart Ross Kelly Bateman Chantelle Hooper Richard Paley Ross D. Houston Tim P. Bean Laboratory Replication of Ostreid Herpes Virus (OsHV-1) Using Pacific Oyster Tissue Explants |
topic_facet |
oyster tissue culture explants Microbiology QR1-502 |
description |
Pacific oysters ( Crassostrea or Magallana gigas ) are one of the most economically important aquaculture species globally. Over the past two decades, ostreid herpesvirus (OsHV-1) has become a major pathogen of cultured Pacific oysters, resulting in widespread mortality with a global distribution. Experimental use of OsHV-1 is challenging for many reasons, including both complexity of host–pathogen dynamics and a lack of functioning model systems. The goal of this study was to improve the tools available for working with OsHV-1 in both whole animals and in tissue explants established from oysters maintained in controlled laboratory conditions. Tissue explants were taken from oysters originating from two different sources that have different levels of mortality in experimental OsHV-1 infections and were exposed to OsHV-1. A whole-animal infection experiment was run concurrently as a comparison. Quantitative PCR and electron microscopy were used to confirm that the explants were capable of replicating OsHV-1. Furthermore, the quantitative PCR results suggest that the source of the oysters was significant in determining the outcome of infection in the explants, supporting the validity of the explant model for OsHV-1 infection. This tissue explant approach for studying OsHV-1 allows for the control of confounding factors in the disease outcome that is not possible in whole-animal experiments, providing a new tool for the study of OsHV-1 in Pacific oysters. |
format |
Article in Journal/Newspaper |
author |
Robert W. A. Potts Tim Regan Stuart Ross Kelly Bateman Chantelle Hooper Richard Paley Ross D. Houston Tim P. Bean |
author_facet |
Robert W. A. Potts Tim Regan Stuart Ross Kelly Bateman Chantelle Hooper Richard Paley Ross D. Houston Tim P. Bean |
author_sort |
Robert W. A. Potts |
title |
Laboratory Replication of Ostreid Herpes Virus (OsHV-1) Using Pacific Oyster Tissue Explants |
title_short |
Laboratory Replication of Ostreid Herpes Virus (OsHV-1) Using Pacific Oyster Tissue Explants |
title_full |
Laboratory Replication of Ostreid Herpes Virus (OsHV-1) Using Pacific Oyster Tissue Explants |
title_fullStr |
Laboratory Replication of Ostreid Herpes Virus (OsHV-1) Using Pacific Oyster Tissue Explants |
title_full_unstemmed |
Laboratory Replication of Ostreid Herpes Virus (OsHV-1) Using Pacific Oyster Tissue Explants |
title_sort |
laboratory replication of ostreid herpes virus (oshv-1) using pacific oyster tissue explants |
publisher |
MDPI AG |
publishDate |
2024 |
url |
https://doi.org/10.3390/v16081343 https://doaj.org/article/dbfc63dd81c94a49aaad1cfb25f95d39 |
genre |
Pacific oyster |
genre_facet |
Pacific oyster |
op_source |
Viruses, Vol 16, Iss 8, p 1343 (2024) |
op_relation |
https://www.mdpi.com/1999-4915/16/8/1343 https://doaj.org/toc/1999-4915 doi:10.3390/v16081343 1999-4915 https://doaj.org/article/dbfc63dd81c94a49aaad1cfb25f95d39 |
op_doi |
https://doi.org/10.3390/v16081343 |
container_title |
Viruses |
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16 |
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8 |
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1343 |
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1810470496359153664 |