A robust pooled testing approach to expand COVID-19 screening capacity.
Limited testing capacity for COVID-19 has hampered the pandemic response. Pooling is a testing method wherein samples from specimens (e.g., swabs) from multiple subjects are combined into a pool and screened with a single test. If the pool tests positive, then new samples from the collected specimen...
Published in: | PLOS ONE |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2021
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pone.0246285 https://doaj.org/article/dbdb1dd0ceaa4c2babc33a74905eaba4 |
id |
ftdoajarticles:oai:doaj.org/article:dbdb1dd0ceaa4c2babc33a74905eaba4 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:dbdb1dd0ceaa4c2babc33a74905eaba4 2023-05-15T16:52:07+02:00 A robust pooled testing approach to expand COVID-19 screening capacity. Douglas R Bish Ebru K Bish Hussein El-Hajj Hrayer Aprahamian 2021-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0246285 https://doaj.org/article/dbdb1dd0ceaa4c2babc33a74905eaba4 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pone.0246285 https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0246285 https://doaj.org/article/dbdb1dd0ceaa4c2babc33a74905eaba4 PLoS ONE, Vol 16, Iss 2, p e0246285 (2021) Medicine R Science Q article 2021 ftdoajarticles https://doi.org/10.1371/journal.pone.0246285 2022-12-31T12:59:02Z Limited testing capacity for COVID-19 has hampered the pandemic response. Pooling is a testing method wherein samples from specimens (e.g., swabs) from multiple subjects are combined into a pool and screened with a single test. If the pool tests positive, then new samples from the collected specimens are individually tested, while if the pool tests negative, the subjects are classified as negative for the disease. Pooling can substantially expand COVID-19 testing capacity and throughput, without requiring additional resources. We develop a mathematical model to determine the best pool size for different risk groups, based on each group's estimated COVID-19 prevalence. Our approach takes into consideration the sensitivity and specificity of the test, and a dynamic and uncertain prevalence, and provides a robust pool size for each group. For practical relevance, we also develop a companion COVID-19 pooling design tool (through a spread sheet). To demonstrate the potential value of pooling, we study COVID-19 screening using testing data from Iceland for the period, February-28-2020 to June-14-2020, for subjects stratified into high- and low-risk groups. We implement the robust pooling strategy within a sequential framework, which updates pool sizes each week, for each risk group, based on prior week's testing data. Robust pooling reduces the number of tests, over individual testing, by 88.5% to 90.2%, and 54.2% to 61.9%, respectively, for the low-risk and high-risk groups (based on test sensitivity values in the range [0.71, 0.98] as reported in the literature). This results in much shorter times, on average, to get the test results compared to individual testing (due to the higher testing throughput), and also allows for expanded screening to cover more individuals. Thus, robust pooling can potentially be a valuable strategy for COVID-19 screening. Article in Journal/Newspaper Iceland Directory of Open Access Journals: DOAJ Articles PLOS ONE 16 2 e0246285 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Douglas R Bish Ebru K Bish Hussein El-Hajj Hrayer Aprahamian A robust pooled testing approach to expand COVID-19 screening capacity. |
topic_facet |
Medicine R Science Q |
description |
Limited testing capacity for COVID-19 has hampered the pandemic response. Pooling is a testing method wherein samples from specimens (e.g., swabs) from multiple subjects are combined into a pool and screened with a single test. If the pool tests positive, then new samples from the collected specimens are individually tested, while if the pool tests negative, the subjects are classified as negative for the disease. Pooling can substantially expand COVID-19 testing capacity and throughput, without requiring additional resources. We develop a mathematical model to determine the best pool size for different risk groups, based on each group's estimated COVID-19 prevalence. Our approach takes into consideration the sensitivity and specificity of the test, and a dynamic and uncertain prevalence, and provides a robust pool size for each group. For practical relevance, we also develop a companion COVID-19 pooling design tool (through a spread sheet). To demonstrate the potential value of pooling, we study COVID-19 screening using testing data from Iceland for the period, February-28-2020 to June-14-2020, for subjects stratified into high- and low-risk groups. We implement the robust pooling strategy within a sequential framework, which updates pool sizes each week, for each risk group, based on prior week's testing data. Robust pooling reduces the number of tests, over individual testing, by 88.5% to 90.2%, and 54.2% to 61.9%, respectively, for the low-risk and high-risk groups (based on test sensitivity values in the range [0.71, 0.98] as reported in the literature). This results in much shorter times, on average, to get the test results compared to individual testing (due to the higher testing throughput), and also allows for expanded screening to cover more individuals. Thus, robust pooling can potentially be a valuable strategy for COVID-19 screening. |
format |
Article in Journal/Newspaper |
author |
Douglas R Bish Ebru K Bish Hussein El-Hajj Hrayer Aprahamian |
author_facet |
Douglas R Bish Ebru K Bish Hussein El-Hajj Hrayer Aprahamian |
author_sort |
Douglas R Bish |
title |
A robust pooled testing approach to expand COVID-19 screening capacity. |
title_short |
A robust pooled testing approach to expand COVID-19 screening capacity. |
title_full |
A robust pooled testing approach to expand COVID-19 screening capacity. |
title_fullStr |
A robust pooled testing approach to expand COVID-19 screening capacity. |
title_full_unstemmed |
A robust pooled testing approach to expand COVID-19 screening capacity. |
title_sort |
robust pooled testing approach to expand covid-19 screening capacity. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pone.0246285 https://doaj.org/article/dbdb1dd0ceaa4c2babc33a74905eaba4 |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
PLoS ONE, Vol 16, Iss 2, p e0246285 (2021) |
op_relation |
https://doi.org/10.1371/journal.pone.0246285 https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0246285 https://doaj.org/article/dbdb1dd0ceaa4c2babc33a74905eaba4 |
op_doi |
https://doi.org/10.1371/journal.pone.0246285 |
container_title |
PLOS ONE |
container_volume |
16 |
container_issue |
2 |
container_start_page |
e0246285 |
_version_ |
1766042239262261248 |