Differential Gene Expression and Infection Profiles of Cutaneous and Mucosal Leishmania braziliensis Isolates from the Same Patient.
Leishmaniasis is a complex disease in which clinical outcome depends on factors such as parasite species, host genetics and immunity and vector species. In Brazil, Leishmania (Viannia) braziliensis is a major etiological agent of cutaneous (CL) and mucosal leishmaniasis (MCL), a disfiguring form of...
Published in: | PLOS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2015
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0004018 https://doaj.org/article/dbb7bfe6f6ac4a3b9e56b7ede247314e |
id |
ftdoajarticles:oai:doaj.org/article:dbb7bfe6f6ac4a3b9e56b7ede247314e |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:dbb7bfe6f6ac4a3b9e56b7ede247314e 2023-05-15T15:14:46+02:00 Differential Gene Expression and Infection Profiles of Cutaneous and Mucosal Leishmania braziliensis Isolates from the Same Patient. Eliza V C Alves-Ferreira Juliano S Toledo Arthur H C De Oliveira Tiago R Ferreira Patricia C Ruy Camila F Pinzan Ramon F Santos Viviane Boaventura David Rojo Ángelez López-Gonzálvez Jose C Rosa Coral Barbas Manoel Barral-Netto Aldina Barral Angela K Cruz 2015-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004018 https://doaj.org/article/dbb7bfe6f6ac4a3b9e56b7ede247314e EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4569073?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004018 https://doaj.org/article/dbb7bfe6f6ac4a3b9e56b7ede247314e PLoS Neglected Tropical Diseases, Vol 9, Iss 9, p e0004018 (2015) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2015 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004018 2022-12-31T14:13:26Z Leishmaniasis is a complex disease in which clinical outcome depends on factors such as parasite species, host genetics and immunity and vector species. In Brazil, Leishmania (Viannia) braziliensis is a major etiological agent of cutaneous (CL) and mucosal leishmaniasis (MCL), a disfiguring form of the disease, which occurs in ~10% of L. braziliensis-infected patients. Thus, clinical isolates from patients with CL and MCL may be a relevant source of information to uncover parasite factors contributing to pathogenesis. In this study, we investigated two pairs of L. (V.) braziliensis isolates from mucosal (LbrM) and cutaneous (LbrC) sites of the same patient to identify factors distinguishing parasites that migrate from those that remain at the primary site of infection.We observed no major genomic divergences among the clinical isolates by molecular karyotype and genomic sequencing. RT-PCR revealed that the isolates lacked Leishmania RNA virus (LRV). However, the isolates exhibited distinct in vivo pathogenesis in BALB/c mice; the LbrC isolates were more virulent than the LbrM isolates. Metabolomic analysis revealed significantly increased levels of 14 metabolites in LbrC parasites and 31 metabolites in LbrM parasites that were mainly related to inflammation and chemotaxis. A proteome comparative analysis revealed the overexpression of LbrPGF2S (prostaglandin f2-alpha synthase) and HSP70 in both LbrC isolates. Overexpression of LbrPGF2S in LbrC and LbrM promastigotes led to an increase in infected macrophages and the number of amastigotes per cell at 24-48 h post-infection (p.i.).Despite sharing high similarity at the genome structure and ploidy levels, the parasites exhibited divergent expressed genomes. The proteome and metabolome results indicated differential profiles between the cutaneous and mucosal isolates, primarily related to inflammation and chemotaxis. BALB/c infection revealed that the cutaneous isolates were more virulent than the mucosal parasites. Furthermore, our data suggest that the LbrPGF2S ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 9 9 e0004018 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Eliza V C Alves-Ferreira Juliano S Toledo Arthur H C De Oliveira Tiago R Ferreira Patricia C Ruy Camila F Pinzan Ramon F Santos Viviane Boaventura David Rojo Ángelez López-Gonzálvez Jose C Rosa Coral Barbas Manoel Barral-Netto Aldina Barral Angela K Cruz Differential Gene Expression and Infection Profiles of Cutaneous and Mucosal Leishmania braziliensis Isolates from the Same Patient. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Leishmaniasis is a complex disease in which clinical outcome depends on factors such as parasite species, host genetics and immunity and vector species. In Brazil, Leishmania (Viannia) braziliensis is a major etiological agent of cutaneous (CL) and mucosal leishmaniasis (MCL), a disfiguring form of the disease, which occurs in ~10% of L. braziliensis-infected patients. Thus, clinical isolates from patients with CL and MCL may be a relevant source of information to uncover parasite factors contributing to pathogenesis. In this study, we investigated two pairs of L. (V.) braziliensis isolates from mucosal (LbrM) and cutaneous (LbrC) sites of the same patient to identify factors distinguishing parasites that migrate from those that remain at the primary site of infection.We observed no major genomic divergences among the clinical isolates by molecular karyotype and genomic sequencing. RT-PCR revealed that the isolates lacked Leishmania RNA virus (LRV). However, the isolates exhibited distinct in vivo pathogenesis in BALB/c mice; the LbrC isolates were more virulent than the LbrM isolates. Metabolomic analysis revealed significantly increased levels of 14 metabolites in LbrC parasites and 31 metabolites in LbrM parasites that were mainly related to inflammation and chemotaxis. A proteome comparative analysis revealed the overexpression of LbrPGF2S (prostaglandin f2-alpha synthase) and HSP70 in both LbrC isolates. Overexpression of LbrPGF2S in LbrC and LbrM promastigotes led to an increase in infected macrophages and the number of amastigotes per cell at 24-48 h post-infection (p.i.).Despite sharing high similarity at the genome structure and ploidy levels, the parasites exhibited divergent expressed genomes. The proteome and metabolome results indicated differential profiles between the cutaneous and mucosal isolates, primarily related to inflammation and chemotaxis. BALB/c infection revealed that the cutaneous isolates were more virulent than the mucosal parasites. Furthermore, our data suggest that the LbrPGF2S ... |
format |
Article in Journal/Newspaper |
author |
Eliza V C Alves-Ferreira Juliano S Toledo Arthur H C De Oliveira Tiago R Ferreira Patricia C Ruy Camila F Pinzan Ramon F Santos Viviane Boaventura David Rojo Ángelez López-Gonzálvez Jose C Rosa Coral Barbas Manoel Barral-Netto Aldina Barral Angela K Cruz |
author_facet |
Eliza V C Alves-Ferreira Juliano S Toledo Arthur H C De Oliveira Tiago R Ferreira Patricia C Ruy Camila F Pinzan Ramon F Santos Viviane Boaventura David Rojo Ángelez López-Gonzálvez Jose C Rosa Coral Barbas Manoel Barral-Netto Aldina Barral Angela K Cruz |
author_sort |
Eliza V C Alves-Ferreira |
title |
Differential Gene Expression and Infection Profiles of Cutaneous and Mucosal Leishmania braziliensis Isolates from the Same Patient. |
title_short |
Differential Gene Expression and Infection Profiles of Cutaneous and Mucosal Leishmania braziliensis Isolates from the Same Patient. |
title_full |
Differential Gene Expression and Infection Profiles of Cutaneous and Mucosal Leishmania braziliensis Isolates from the Same Patient. |
title_fullStr |
Differential Gene Expression and Infection Profiles of Cutaneous and Mucosal Leishmania braziliensis Isolates from the Same Patient. |
title_full_unstemmed |
Differential Gene Expression and Infection Profiles of Cutaneous and Mucosal Leishmania braziliensis Isolates from the Same Patient. |
title_sort |
differential gene expression and infection profiles of cutaneous and mucosal leishmania braziliensis isolates from the same patient. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pntd.0004018 https://doaj.org/article/dbb7bfe6f6ac4a3b9e56b7ede247314e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 9, Iss 9, p e0004018 (2015) |
op_relation |
http://europepmc.org/articles/PMC4569073?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004018 https://doaj.org/article/dbb7bfe6f6ac4a3b9e56b7ede247314e |
op_doi |
https://doi.org/10.1371/journal.pntd.0004018 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
9 |
container_issue |
9 |
container_start_page |
e0004018 |
_version_ |
1766345181754294272 |