Chromosome Distribution of Highly Conserved Tandemly Arranged Repetitive DNAs in the Siberian Sturgeon ( Acipenser baerii )
Polyploid genomes present a challenge for cytogenetic and genomic studies, due to the high number of similar size chromosomes and the simultaneous presence of hardly distinguishable paralogous elements. The karyotype of the Siberian sturgeon ( Acipenser baerii ) contains around 250 chromosomes and i...
Published in: | Genes |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
MDPI AG
2020
|
Subjects: | |
Online Access: | https://doi.org/10.3390/genes11111375 https://doaj.org/article/dba4e9eaafc247eca0c6485c68d647ba |
id |
ftdoajarticles:oai:doaj.org/article:dba4e9eaafc247eca0c6485c68d647ba |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:dba4e9eaafc247eca0c6485c68d647ba 2023-05-15T13:01:37+02:00 Chromosome Distribution of Highly Conserved Tandemly Arranged Repetitive DNAs in the Siberian Sturgeon ( Acipenser baerii ) Larisa S. Biltueva Dmitry Yu. Prokopov Svetlana A. Romanenko Elena A. Interesova Manfred Schartl Vladimir A. Trifonov 2020-11-01T00:00:00Z https://doi.org/10.3390/genes11111375 https://doaj.org/article/dba4e9eaafc247eca0c6485c68d647ba EN eng MDPI AG https://www.mdpi.com/2073-4425/11/11/1375 https://doaj.org/toc/2073-4425 doi:10.3390/genes11111375 2073-4425 https://doaj.org/article/dba4e9eaafc247eca0c6485c68d647ba Genes, Vol 11, Iss 1375, p 1375 (2020) Acipenser baerii sturgeon karyotype whole-genome duplication paralogs polyploidy acipenserid minisatellite Genetics QH426-470 article 2020 ftdoajarticles https://doi.org/10.3390/genes11111375 2022-12-31T14:24:01Z Polyploid genomes present a challenge for cytogenetic and genomic studies, due to the high number of similar size chromosomes and the simultaneous presence of hardly distinguishable paralogous elements. The karyotype of the Siberian sturgeon ( Acipenser baerii ) contains around 250 chromosomes and is remarkable for the presence of paralogs from two rounds of whole-genome duplications (WGD). In this study, we applied the sterlet-derived acipenserid satDNA-based whole chromosome-specific probes to analyze the Siberian sturgeon karyotype. We demonstrate that the last genome duplication event in the Siberian sturgeon was accompanied by the simultaneous expansion of several repetitive DNA families. Some of the repetitive probes serve as good cytogenetic markers distinguishing paralogous chromosomes and detecting ancestral syntenic regions, which underwent fusions and fissions. The tendency of minisatellite specificity for chromosome size groups previously observed in the sterlet genome is also visible in the Siberian sturgeon. We provide an initial physical chromosome map of the Siberian sturgeon genome supported by molecular markers. The application of these data will facilitate genomic studies in other recent polyploid sturgeon species. Article in Journal/Newspaper Acipenser baerii Siberian sturgeon Directory of Open Access Journals: DOAJ Articles Genes 11 11 1375 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Acipenser baerii sturgeon karyotype whole-genome duplication paralogs polyploidy acipenserid minisatellite Genetics QH426-470 |
spellingShingle |
Acipenser baerii sturgeon karyotype whole-genome duplication paralogs polyploidy acipenserid minisatellite Genetics QH426-470 Larisa S. Biltueva Dmitry Yu. Prokopov Svetlana A. Romanenko Elena A. Interesova Manfred Schartl Vladimir A. Trifonov Chromosome Distribution of Highly Conserved Tandemly Arranged Repetitive DNAs in the Siberian Sturgeon ( Acipenser baerii ) |
topic_facet |
Acipenser baerii sturgeon karyotype whole-genome duplication paralogs polyploidy acipenserid minisatellite Genetics QH426-470 |
description |
Polyploid genomes present a challenge for cytogenetic and genomic studies, due to the high number of similar size chromosomes and the simultaneous presence of hardly distinguishable paralogous elements. The karyotype of the Siberian sturgeon ( Acipenser baerii ) contains around 250 chromosomes and is remarkable for the presence of paralogs from two rounds of whole-genome duplications (WGD). In this study, we applied the sterlet-derived acipenserid satDNA-based whole chromosome-specific probes to analyze the Siberian sturgeon karyotype. We demonstrate that the last genome duplication event in the Siberian sturgeon was accompanied by the simultaneous expansion of several repetitive DNA families. Some of the repetitive probes serve as good cytogenetic markers distinguishing paralogous chromosomes and detecting ancestral syntenic regions, which underwent fusions and fissions. The tendency of minisatellite specificity for chromosome size groups previously observed in the sterlet genome is also visible in the Siberian sturgeon. We provide an initial physical chromosome map of the Siberian sturgeon genome supported by molecular markers. The application of these data will facilitate genomic studies in other recent polyploid sturgeon species. |
format |
Article in Journal/Newspaper |
author |
Larisa S. Biltueva Dmitry Yu. Prokopov Svetlana A. Romanenko Elena A. Interesova Manfred Schartl Vladimir A. Trifonov |
author_facet |
Larisa S. Biltueva Dmitry Yu. Prokopov Svetlana A. Romanenko Elena A. Interesova Manfred Schartl Vladimir A. Trifonov |
author_sort |
Larisa S. Biltueva |
title |
Chromosome Distribution of Highly Conserved Tandemly Arranged Repetitive DNAs in the Siberian Sturgeon ( Acipenser baerii ) |
title_short |
Chromosome Distribution of Highly Conserved Tandemly Arranged Repetitive DNAs in the Siberian Sturgeon ( Acipenser baerii ) |
title_full |
Chromosome Distribution of Highly Conserved Tandemly Arranged Repetitive DNAs in the Siberian Sturgeon ( Acipenser baerii ) |
title_fullStr |
Chromosome Distribution of Highly Conserved Tandemly Arranged Repetitive DNAs in the Siberian Sturgeon ( Acipenser baerii ) |
title_full_unstemmed |
Chromosome Distribution of Highly Conserved Tandemly Arranged Repetitive DNAs in the Siberian Sturgeon ( Acipenser baerii ) |
title_sort |
chromosome distribution of highly conserved tandemly arranged repetitive dnas in the siberian sturgeon ( acipenser baerii ) |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/genes11111375 https://doaj.org/article/dba4e9eaafc247eca0c6485c68d647ba |
genre |
Acipenser baerii Siberian sturgeon |
genre_facet |
Acipenser baerii Siberian sturgeon |
op_source |
Genes, Vol 11, Iss 1375, p 1375 (2020) |
op_relation |
https://www.mdpi.com/2073-4425/11/11/1375 https://doaj.org/toc/2073-4425 doi:10.3390/genes11111375 2073-4425 https://doaj.org/article/dba4e9eaafc247eca0c6485c68d647ba |
op_doi |
https://doi.org/10.3390/genes11111375 |
container_title |
Genes |
container_volume |
11 |
container_issue |
11 |
container_start_page |
1375 |
_version_ |
1766281809555881984 |