Herbivore-induced chemical and molecular responses of the kelps Laminaria digitata and Lessonia spicata.
Kelps are founding species of temperate marine ecosystems, living in intertidal coastal areas where they are often challenged by generalist and specialist herbivores. As most sessile organisms, kelps develop defensive strategies to restrain grazing damage and preserve their own fitness during intera...
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ftdoajarticles:oai:doaj.org/article:dba1f1454bb447e1a09e27caa8cd54eb 2023-05-15T17:35:32+02:00 Herbivore-induced chemical and molecular responses of the kelps Laminaria digitata and Lessonia spicata. Andrés Ritter Léa Cabioch Loraine Brillet-Guéguen Erwan Corre Audrey Cosse Laurence Dartevelle Harold Duruflé Carina Fasshauer Sophie Goulitquer François Thomas Juan A Correa Philippe Potin Sylvain Faugeron Catherine Leblanc 2017-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0173315 https://doaj.org/article/dba1f1454bb447e1a09e27caa8cd54eb EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5333891?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0173315 https://doaj.org/article/dba1f1454bb447e1a09e27caa8cd54eb PLoS ONE, Vol 12, Iss 3, p e0173315 (2017) Medicine R Science Q article 2017 ftdoajarticles https://doi.org/10.1371/journal.pone.0173315 2022-12-31T03:55:23Z Kelps are founding species of temperate marine ecosystems, living in intertidal coastal areas where they are often challenged by generalist and specialist herbivores. As most sessile organisms, kelps develop defensive strategies to restrain grazing damage and preserve their own fitness during interactions with herbivores. To decipher some inducible defense and signaling mechanisms, we carried out metabolome and transcriptome analyses in two emblematic kelp species, Lessonia spicata from South Pacific coasts and Laminaria digitata from North Atlantic, when challenged with their main specialist herbivores. Mass spectrometry based metabolomics revealed large metabolic changes induced in these two brown algae following challenges with their own specialist herbivores. Targeted metabolic profiling of L. spicata further showed that free fatty acid (FFA) and amino acid (AA) metabolisms were particularly regulated under grazing. An early stress response was illustrated by the accumulation of Sulphur containing amino acids in the first twelve hours of herbivory pressure. At latter time periods (after 24 hours), we observed FFA liberation and eicosanoid oxylipins synthesis likely representing metabolites related to stress. Global transcriptomic analysis identified sets of candidate genes specifically induced by grazing in both kelps. qPCR analysis of the top candidate genes during a 48-hours time course validated the results. Most of these genes were particularly activated by herbivore challenge after 24 hours, suggesting that transcriptional reprogramming could be operated at this time period. We demonstrated the potential utility of these genes as molecular markers for herbivory by measuring their inductions in grazed individuals of field harvested L. digitata and L. spicata. By unravelling the regulation of some metabolites and genes following grazing pressure in two kelps representative of the two hemispheres, this work contributes to provide a set of herbivore-induced chemical and molecular responses in kelp species, ... Article in Journal/Newspaper North Atlantic Directory of Open Access Journals: DOAJ Articles Pacific PLOS ONE 12 3 e0173315 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Andrés Ritter Léa Cabioch Loraine Brillet-Guéguen Erwan Corre Audrey Cosse Laurence Dartevelle Harold Duruflé Carina Fasshauer Sophie Goulitquer François Thomas Juan A Correa Philippe Potin Sylvain Faugeron Catherine Leblanc Herbivore-induced chemical and molecular responses of the kelps Laminaria digitata and Lessonia spicata. |
topic_facet |
Medicine R Science Q |
description |
Kelps are founding species of temperate marine ecosystems, living in intertidal coastal areas where they are often challenged by generalist and specialist herbivores. As most sessile organisms, kelps develop defensive strategies to restrain grazing damage and preserve their own fitness during interactions with herbivores. To decipher some inducible defense and signaling mechanisms, we carried out metabolome and transcriptome analyses in two emblematic kelp species, Lessonia spicata from South Pacific coasts and Laminaria digitata from North Atlantic, when challenged with their main specialist herbivores. Mass spectrometry based metabolomics revealed large metabolic changes induced in these two brown algae following challenges with their own specialist herbivores. Targeted metabolic profiling of L. spicata further showed that free fatty acid (FFA) and amino acid (AA) metabolisms were particularly regulated under grazing. An early stress response was illustrated by the accumulation of Sulphur containing amino acids in the first twelve hours of herbivory pressure. At latter time periods (after 24 hours), we observed FFA liberation and eicosanoid oxylipins synthesis likely representing metabolites related to stress. Global transcriptomic analysis identified sets of candidate genes specifically induced by grazing in both kelps. qPCR analysis of the top candidate genes during a 48-hours time course validated the results. Most of these genes were particularly activated by herbivore challenge after 24 hours, suggesting that transcriptional reprogramming could be operated at this time period. We demonstrated the potential utility of these genes as molecular markers for herbivory by measuring their inductions in grazed individuals of field harvested L. digitata and L. spicata. By unravelling the regulation of some metabolites and genes following grazing pressure in two kelps representative of the two hemispheres, this work contributes to provide a set of herbivore-induced chemical and molecular responses in kelp species, ... |
format |
Article in Journal/Newspaper |
author |
Andrés Ritter Léa Cabioch Loraine Brillet-Guéguen Erwan Corre Audrey Cosse Laurence Dartevelle Harold Duruflé Carina Fasshauer Sophie Goulitquer François Thomas Juan A Correa Philippe Potin Sylvain Faugeron Catherine Leblanc |
author_facet |
Andrés Ritter Léa Cabioch Loraine Brillet-Guéguen Erwan Corre Audrey Cosse Laurence Dartevelle Harold Duruflé Carina Fasshauer Sophie Goulitquer François Thomas Juan A Correa Philippe Potin Sylvain Faugeron Catherine Leblanc |
author_sort |
Andrés Ritter |
title |
Herbivore-induced chemical and molecular responses of the kelps Laminaria digitata and Lessonia spicata. |
title_short |
Herbivore-induced chemical and molecular responses of the kelps Laminaria digitata and Lessonia spicata. |
title_full |
Herbivore-induced chemical and molecular responses of the kelps Laminaria digitata and Lessonia spicata. |
title_fullStr |
Herbivore-induced chemical and molecular responses of the kelps Laminaria digitata and Lessonia spicata. |
title_full_unstemmed |
Herbivore-induced chemical and molecular responses of the kelps Laminaria digitata and Lessonia spicata. |
title_sort |
herbivore-induced chemical and molecular responses of the kelps laminaria digitata and lessonia spicata. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pone.0173315 https://doaj.org/article/dba1f1454bb447e1a09e27caa8cd54eb |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
PLoS ONE, Vol 12, Iss 3, p e0173315 (2017) |
op_relation |
http://europepmc.org/articles/PMC5333891?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0173315 https://doaj.org/article/dba1f1454bb447e1a09e27caa8cd54eb |
op_doi |
https://doi.org/10.1371/journal.pone.0173315 |
container_title |
PLOS ONE |
container_volume |
12 |
container_issue |
3 |
container_start_page |
e0173315 |
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1766134727923728384 |