Polyphasic Analysis Reveals Potential Petroleum Hydrocarbon Degradation and Biosurfactant Production by Rare Biosphere Thermophilic Bacteria From Deception Island, an Active Antarctic Volcano
Extreme temperature gradients in polar volcanoes are capable of selecting different types of extremophiles. Deception Island is a marine stratovolcano located in maritime Antarctica. The volcano has pronounced temperature gradients over very short distances, from as high as 100°C in the fumaroles to...
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2022
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Online Access: | https://doi.org/10.3389/fmicb.2022.885557 https://doaj.org/article/db9580230cd04699bec19f7c0e8b05c6 |
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ftdoajarticles:oai:doaj.org/article:db9580230cd04699bec19f7c0e8b05c6 2023-05-15T14:04:27+02:00 Polyphasic Analysis Reveals Potential Petroleum Hydrocarbon Degradation and Biosurfactant Production by Rare Biosphere Thermophilic Bacteria From Deception Island, an Active Antarctic Volcano Júnia Schultz Isabella Campelo Vilardi Argentino René Kallies Ulisses Nunes da Rocha Alexandre Soares Rosado 2022-05-01T00:00:00Z https://doi.org/10.3389/fmicb.2022.885557 https://doaj.org/article/db9580230cd04699bec19f7c0e8b05c6 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fmicb.2022.885557/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2022.885557 https://doaj.org/article/db9580230cd04699bec19f7c0e8b05c6 Frontiers in Microbiology, Vol 13 (2022) extremophiles thermophiles polar volcano Antarctica biosurfactants oil degradation Microbiology QR1-502 article 2022 ftdoajarticles https://doi.org/10.3389/fmicb.2022.885557 2022-12-31T02:33:12Z Extreme temperature gradients in polar volcanoes are capable of selecting different types of extremophiles. Deception Island is a marine stratovolcano located in maritime Antarctica. The volcano has pronounced temperature gradients over very short distances, from as high as 100°C in the fumaroles to subzero next to the glaciers. These characteristics make Deception a promising source of a variety of bioproducts for use in different biotechnological areas. In this study, we isolated thermophilic bacteria from sediments in fumaroles at two geothermal sites on Deception Island with temperatures between 50 and 100°C, to evaluate the potential capacity of these bacteria to degrade petroleum hydrocarbons and produce biosurfactants under thermophilic conditions. We isolated 126 thermophilic bacterial strains and identified them molecularly as members of genera Geobacillus, Anoxybacillus, and Brevibacillus (all in phylum Firmicutes). Seventy-six strains grew in a culture medium supplemented with crude oil as the only carbon source, and 30 of them showed particularly good results for oil degradation. Of 50 strains tested for biosurfactant production, 13 showed good results, with an emulsification index of 50% or higher of a petroleum hydrocarbon source (crude oil and diesel), emulsification stability at 100°C, and positive results in drop-collapse, oil spreading, and hemolytic activity tests. Four of these isolates showed great capability of degrade crude oil: FB2_38 (Geobacillus), FB3_54 (Geobacillus), FB4_88 (Anoxybacillus), and WB1_122 (Geobacillus). Genomic analysis of the oil-degrading and biosurfactant-producer strain FB4_88 identified it as Anoxybacillus flavithermus, with a high genetic and functional diversity potential for biotechnological applications. These initial culturomic and genomic data suggest that thermophilic bacteria from this Antarctic volcano have potential applications in the petroleum industry, for bioremediation in extreme environments and for microbial enhanced oil recovery (MEOR) in ... Article in Journal/Newspaper Antarc* Antarctic Antarctica Deception Island Directory of Open Access Journals: DOAJ Articles Antarctic Deception Island ENVELOPE(-60.633,-60.633,-62.950,-62.950) Frontiers in Microbiology 13 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
extremophiles thermophiles polar volcano Antarctica biosurfactants oil degradation Microbiology QR1-502 |
spellingShingle |
extremophiles thermophiles polar volcano Antarctica biosurfactants oil degradation Microbiology QR1-502 Júnia Schultz Isabella Campelo Vilardi Argentino René Kallies Ulisses Nunes da Rocha Alexandre Soares Rosado Polyphasic Analysis Reveals Potential Petroleum Hydrocarbon Degradation and Biosurfactant Production by Rare Biosphere Thermophilic Bacteria From Deception Island, an Active Antarctic Volcano |
topic_facet |
extremophiles thermophiles polar volcano Antarctica biosurfactants oil degradation Microbiology QR1-502 |
description |
Extreme temperature gradients in polar volcanoes are capable of selecting different types of extremophiles. Deception Island is a marine stratovolcano located in maritime Antarctica. The volcano has pronounced temperature gradients over very short distances, from as high as 100°C in the fumaroles to subzero next to the glaciers. These characteristics make Deception a promising source of a variety of bioproducts for use in different biotechnological areas. In this study, we isolated thermophilic bacteria from sediments in fumaroles at two geothermal sites on Deception Island with temperatures between 50 and 100°C, to evaluate the potential capacity of these bacteria to degrade petroleum hydrocarbons and produce biosurfactants under thermophilic conditions. We isolated 126 thermophilic bacterial strains and identified them molecularly as members of genera Geobacillus, Anoxybacillus, and Brevibacillus (all in phylum Firmicutes). Seventy-six strains grew in a culture medium supplemented with crude oil as the only carbon source, and 30 of them showed particularly good results for oil degradation. Of 50 strains tested for biosurfactant production, 13 showed good results, with an emulsification index of 50% or higher of a petroleum hydrocarbon source (crude oil and diesel), emulsification stability at 100°C, and positive results in drop-collapse, oil spreading, and hemolytic activity tests. Four of these isolates showed great capability of degrade crude oil: FB2_38 (Geobacillus), FB3_54 (Geobacillus), FB4_88 (Anoxybacillus), and WB1_122 (Geobacillus). Genomic analysis of the oil-degrading and biosurfactant-producer strain FB4_88 identified it as Anoxybacillus flavithermus, with a high genetic and functional diversity potential for biotechnological applications. These initial culturomic and genomic data suggest that thermophilic bacteria from this Antarctic volcano have potential applications in the petroleum industry, for bioremediation in extreme environments and for microbial enhanced oil recovery (MEOR) in ... |
format |
Article in Journal/Newspaper |
author |
Júnia Schultz Isabella Campelo Vilardi Argentino René Kallies Ulisses Nunes da Rocha Alexandre Soares Rosado |
author_facet |
Júnia Schultz Isabella Campelo Vilardi Argentino René Kallies Ulisses Nunes da Rocha Alexandre Soares Rosado |
author_sort |
Júnia Schultz |
title |
Polyphasic Analysis Reveals Potential Petroleum Hydrocarbon Degradation and Biosurfactant Production by Rare Biosphere Thermophilic Bacteria From Deception Island, an Active Antarctic Volcano |
title_short |
Polyphasic Analysis Reveals Potential Petroleum Hydrocarbon Degradation and Biosurfactant Production by Rare Biosphere Thermophilic Bacteria From Deception Island, an Active Antarctic Volcano |
title_full |
Polyphasic Analysis Reveals Potential Petroleum Hydrocarbon Degradation and Biosurfactant Production by Rare Biosphere Thermophilic Bacteria From Deception Island, an Active Antarctic Volcano |
title_fullStr |
Polyphasic Analysis Reveals Potential Petroleum Hydrocarbon Degradation and Biosurfactant Production by Rare Biosphere Thermophilic Bacteria From Deception Island, an Active Antarctic Volcano |
title_full_unstemmed |
Polyphasic Analysis Reveals Potential Petroleum Hydrocarbon Degradation and Biosurfactant Production by Rare Biosphere Thermophilic Bacteria From Deception Island, an Active Antarctic Volcano |
title_sort |
polyphasic analysis reveals potential petroleum hydrocarbon degradation and biosurfactant production by rare biosphere thermophilic bacteria from deception island, an active antarctic volcano |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
https://doi.org/10.3389/fmicb.2022.885557 https://doaj.org/article/db9580230cd04699bec19f7c0e8b05c6 |
long_lat |
ENVELOPE(-60.633,-60.633,-62.950,-62.950) |
geographic |
Antarctic Deception Island |
geographic_facet |
Antarctic Deception Island |
genre |
Antarc* Antarctic Antarctica Deception Island |
genre_facet |
Antarc* Antarctic Antarctica Deception Island |
op_source |
Frontiers in Microbiology, Vol 13 (2022) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fmicb.2022.885557/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2022.885557 https://doaj.org/article/db9580230cd04699bec19f7c0e8b05c6 |
op_doi |
https://doi.org/10.3389/fmicb.2022.885557 |
container_title |
Frontiers in Microbiology |
container_volume |
13 |
_version_ |
1766275542281093120 |