Insights into the Jasmonate Signaling in Basal Land Plant Revealed by the Multi-Omics Analysis of an Antarctic Moss Pohlia nutans Treated with OPDA
12-oxo-phytodienoic acid (OPDA) is a biosynthetic precursor of jasmonic acid and triggers multiple biological processes from plant development to stress responses. However, the OPDA signaling and relevant regulatory networks were largely unknown in basal land plants. Using an integrated multi-omics...
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ftdoajarticles:oai:doaj.org/article:db93369256db4f498ebd9244c67358d9 2023-05-15T13:42:23+02:00 Insights into the Jasmonate Signaling in Basal Land Plant Revealed by the Multi-Omics Analysis of an Antarctic Moss Pohlia nutans Treated with OPDA Shenghao Liu Tingting Li Pengying Zhang Linlin Zhao Dan Yi Zhaohui Zhang Bailin Cong 2022-11-01T00:00:00Z https://doi.org/10.3390/ijms232113507 https://doaj.org/article/db93369256db4f498ebd9244c67358d9 EN eng MDPI AG https://www.mdpi.com/1422-0067/23/21/13507 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms232113507 1422-0067 1661-6596 https://doaj.org/article/db93369256db4f498ebd9244c67358d9 International Journal of Molecular Sciences, Vol 23, Iss 13507, p 13507 (2022) jasmonate flavonoids metabolomic profiling transcriptomic sequencing bryophytes Biology (General) QH301-705.5 Chemistry QD1-999 article 2022 ftdoajarticles https://doi.org/10.3390/ijms232113507 2022-12-30T19:39:04Z 12-oxo-phytodienoic acid (OPDA) is a biosynthetic precursor of jasmonic acid and triggers multiple biological processes from plant development to stress responses. However, the OPDA signaling and relevant regulatory networks were largely unknown in basal land plants. Using an integrated multi-omics technique, we investigated the global features in metabolites and transcriptional profiles of an Antarctic moss ( Pohlia nutans ) in response to OPDA treatment. We detected 676 metabolites based on the widely targeted metabolomics approach. A total of 82 significantly changed metabolites were observed, including fatty acids, flavonoids, phenolic acids, amino acids and derivatives, and alkaloids. In addition, the transcriptome sequencing was conducted to uncover the global transcriptional profiles. The representative differentially expressed genes were summarized into functions including Ca 2+ signaling, abscisic acid signaling, jasmonate signaling, lipid and fatty acid biosynthesis, transcription factors, antioxidant enzymes, and detoxification proteins. The integrated multi-omics analysis revealed that the pathways of jasmonate and ABA signaling, lipid and fatty acid biosynthesis, and flavonoid biosynthesis might dominate the molecular responses to OPDA. Taken together, these observations provide insights into the molecular evolution of jasmonate signaling and the adaptation mechanisms of Antarctic moss to terrestrial habitats. Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic International Journal of Molecular Sciences 23 21 13507 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
jasmonate flavonoids metabolomic profiling transcriptomic sequencing bryophytes Biology (General) QH301-705.5 Chemistry QD1-999 |
spellingShingle |
jasmonate flavonoids metabolomic profiling transcriptomic sequencing bryophytes Biology (General) QH301-705.5 Chemistry QD1-999 Shenghao Liu Tingting Li Pengying Zhang Linlin Zhao Dan Yi Zhaohui Zhang Bailin Cong Insights into the Jasmonate Signaling in Basal Land Plant Revealed by the Multi-Omics Analysis of an Antarctic Moss Pohlia nutans Treated with OPDA |
topic_facet |
jasmonate flavonoids metabolomic profiling transcriptomic sequencing bryophytes Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
12-oxo-phytodienoic acid (OPDA) is a biosynthetic precursor of jasmonic acid and triggers multiple biological processes from plant development to stress responses. However, the OPDA signaling and relevant regulatory networks were largely unknown in basal land plants. Using an integrated multi-omics technique, we investigated the global features in metabolites and transcriptional profiles of an Antarctic moss ( Pohlia nutans ) in response to OPDA treatment. We detected 676 metabolites based on the widely targeted metabolomics approach. A total of 82 significantly changed metabolites were observed, including fatty acids, flavonoids, phenolic acids, amino acids and derivatives, and alkaloids. In addition, the transcriptome sequencing was conducted to uncover the global transcriptional profiles. The representative differentially expressed genes were summarized into functions including Ca 2+ signaling, abscisic acid signaling, jasmonate signaling, lipid and fatty acid biosynthesis, transcription factors, antioxidant enzymes, and detoxification proteins. The integrated multi-omics analysis revealed that the pathways of jasmonate and ABA signaling, lipid and fatty acid biosynthesis, and flavonoid biosynthesis might dominate the molecular responses to OPDA. Taken together, these observations provide insights into the molecular evolution of jasmonate signaling and the adaptation mechanisms of Antarctic moss to terrestrial habitats. |
format |
Article in Journal/Newspaper |
author |
Shenghao Liu Tingting Li Pengying Zhang Linlin Zhao Dan Yi Zhaohui Zhang Bailin Cong |
author_facet |
Shenghao Liu Tingting Li Pengying Zhang Linlin Zhao Dan Yi Zhaohui Zhang Bailin Cong |
author_sort |
Shenghao Liu |
title |
Insights into the Jasmonate Signaling in Basal Land Plant Revealed by the Multi-Omics Analysis of an Antarctic Moss Pohlia nutans Treated with OPDA |
title_short |
Insights into the Jasmonate Signaling in Basal Land Plant Revealed by the Multi-Omics Analysis of an Antarctic Moss Pohlia nutans Treated with OPDA |
title_full |
Insights into the Jasmonate Signaling in Basal Land Plant Revealed by the Multi-Omics Analysis of an Antarctic Moss Pohlia nutans Treated with OPDA |
title_fullStr |
Insights into the Jasmonate Signaling in Basal Land Plant Revealed by the Multi-Omics Analysis of an Antarctic Moss Pohlia nutans Treated with OPDA |
title_full_unstemmed |
Insights into the Jasmonate Signaling in Basal Land Plant Revealed by the Multi-Omics Analysis of an Antarctic Moss Pohlia nutans Treated with OPDA |
title_sort |
insights into the jasmonate signaling in basal land plant revealed by the multi-omics analysis of an antarctic moss pohlia nutans treated with opda |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/ijms232113507 https://doaj.org/article/db93369256db4f498ebd9244c67358d9 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
International Journal of Molecular Sciences, Vol 23, Iss 13507, p 13507 (2022) |
op_relation |
https://www.mdpi.com/1422-0067/23/21/13507 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms232113507 1422-0067 1661-6596 https://doaj.org/article/db93369256db4f498ebd9244c67358d9 |
op_doi |
https://doi.org/10.3390/ijms232113507 |
container_title |
International Journal of Molecular Sciences |
container_volume |
23 |
container_issue |
21 |
container_start_page |
13507 |
_version_ |
1766167452263120896 |