A novel, sequencing-free strategy for the functional characterization of Taenia solium proteomic fingerprint.
The flatworm Taenia solium causes human and pig cysticercosis. When cysticerci are established in the human central nervous system, they cause neurocysticercosis, a potentially fatal disease. Neurocysticercosis is a persisting public health problem in rural regions of Mexico and other developing cou...
Published in: | PLOS Neglected Tropical Diseases |
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Online Access: | https://doi.org/10.1371/journal.pntd.0009104 https://doaj.org/article/db8c8e7e1d74409c9a7fa9c016faa4b9 |
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ftdoajarticles:oai:doaj.org/article:db8c8e7e1d74409c9a7fa9c016faa4b9 2023-05-15T15:12:48+02:00 A novel, sequencing-free strategy for the functional characterization of Taenia solium proteomic fingerprint. Sandra Gomez-Fuentes Sarah Hernández-de la Fuente Valeria Morales-Ruiz Dina López-Recinos Adrián Guevara-Salinas María Cristina Parada-Colin Clara Espitia Adrián Ochoa-Leyva Filiberto Sánchez Nelly Villalobos Asiel Arce-Sillas Marisela Hernández Silvia Ivonne Mora Gladis Fragoso Edda Sciutto Laura Adalid-Peralta 2021-02-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009104 https://doaj.org/article/db8c8e7e1d74409c9a7fa9c016faa4b9 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009104 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009104 https://doaj.org/article/db8c8e7e1d74409c9a7fa9c016faa4b9 PLoS Neglected Tropical Diseases, Vol 15, Iss 2, p e0009104 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009104 2022-12-31T07:36:41Z The flatworm Taenia solium causes human and pig cysticercosis. When cysticerci are established in the human central nervous system, they cause neurocysticercosis, a potentially fatal disease. Neurocysticercosis is a persisting public health problem in rural regions of Mexico and other developing countries of Latin America, Asia, and Africa, where the infection is endemic. The great variability observed in the phenotypic and genotypic traits of cysticerci result in a great heterogeneity in the patterns of molecules secreted by them within their host. This work is aimed to identify and characterize cysticercal secretion proteins of T. solium cysticerci obtained from 5 naturally infected pigs from Guerrero, Mexico, using 2D-PAGE proteomic analysis. The isoelectric point (IP) and molecular weight (MW) of the spots were identified using the software ImageMaster 2D Platinum v.7.0. Since most secreted proteins are impossible to identify by mass spectrometry (MS) due to their low concentration in the sample, a novel strategy to predict their sequence was applied. In total, 108 conserved and 186 differential proteins were identified in five cysticercus cultures. Interestingly, we predicted the sequence of 14 proteins that were common in four out of five cysticercus cultures, which could be used to design vaccines or diagnostic methods for neurocysticercosis. A functional characterization of all sequences was performed using the algorithms SecretomeP, SignalP, and BlastKOALA. We found a possible link between signal transduction pathways in parasite cells and human cancer due to deregulation in signal transduction pathways. Bioinformatics analysis also demonstrated that the parasite release proteins by an exosome-like mechanism, which could be of biological interest. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 2 e0009104 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Sandra Gomez-Fuentes Sarah Hernández-de la Fuente Valeria Morales-Ruiz Dina López-Recinos Adrián Guevara-Salinas María Cristina Parada-Colin Clara Espitia Adrián Ochoa-Leyva Filiberto Sánchez Nelly Villalobos Asiel Arce-Sillas Marisela Hernández Silvia Ivonne Mora Gladis Fragoso Edda Sciutto Laura Adalid-Peralta A novel, sequencing-free strategy for the functional characterization of Taenia solium proteomic fingerprint. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The flatworm Taenia solium causes human and pig cysticercosis. When cysticerci are established in the human central nervous system, they cause neurocysticercosis, a potentially fatal disease. Neurocysticercosis is a persisting public health problem in rural regions of Mexico and other developing countries of Latin America, Asia, and Africa, where the infection is endemic. The great variability observed in the phenotypic and genotypic traits of cysticerci result in a great heterogeneity in the patterns of molecules secreted by them within their host. This work is aimed to identify and characterize cysticercal secretion proteins of T. solium cysticerci obtained from 5 naturally infected pigs from Guerrero, Mexico, using 2D-PAGE proteomic analysis. The isoelectric point (IP) and molecular weight (MW) of the spots were identified using the software ImageMaster 2D Platinum v.7.0. Since most secreted proteins are impossible to identify by mass spectrometry (MS) due to their low concentration in the sample, a novel strategy to predict their sequence was applied. In total, 108 conserved and 186 differential proteins were identified in five cysticercus cultures. Interestingly, we predicted the sequence of 14 proteins that were common in four out of five cysticercus cultures, which could be used to design vaccines or diagnostic methods for neurocysticercosis. A functional characterization of all sequences was performed using the algorithms SecretomeP, SignalP, and BlastKOALA. We found a possible link between signal transduction pathways in parasite cells and human cancer due to deregulation in signal transduction pathways. Bioinformatics analysis also demonstrated that the parasite release proteins by an exosome-like mechanism, which could be of biological interest. |
format |
Article in Journal/Newspaper |
author |
Sandra Gomez-Fuentes Sarah Hernández-de la Fuente Valeria Morales-Ruiz Dina López-Recinos Adrián Guevara-Salinas María Cristina Parada-Colin Clara Espitia Adrián Ochoa-Leyva Filiberto Sánchez Nelly Villalobos Asiel Arce-Sillas Marisela Hernández Silvia Ivonne Mora Gladis Fragoso Edda Sciutto Laura Adalid-Peralta |
author_facet |
Sandra Gomez-Fuentes Sarah Hernández-de la Fuente Valeria Morales-Ruiz Dina López-Recinos Adrián Guevara-Salinas María Cristina Parada-Colin Clara Espitia Adrián Ochoa-Leyva Filiberto Sánchez Nelly Villalobos Asiel Arce-Sillas Marisela Hernández Silvia Ivonne Mora Gladis Fragoso Edda Sciutto Laura Adalid-Peralta |
author_sort |
Sandra Gomez-Fuentes |
title |
A novel, sequencing-free strategy for the functional characterization of Taenia solium proteomic fingerprint. |
title_short |
A novel, sequencing-free strategy for the functional characterization of Taenia solium proteomic fingerprint. |
title_full |
A novel, sequencing-free strategy for the functional characterization of Taenia solium proteomic fingerprint. |
title_fullStr |
A novel, sequencing-free strategy for the functional characterization of Taenia solium proteomic fingerprint. |
title_full_unstemmed |
A novel, sequencing-free strategy for the functional characterization of Taenia solium proteomic fingerprint. |
title_sort |
novel, sequencing-free strategy for the functional characterization of taenia solium proteomic fingerprint. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009104 https://doaj.org/article/db8c8e7e1d74409c9a7fa9c016faa4b9 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 2, p e0009104 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009104 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009104 https://doaj.org/article/db8c8e7e1d74409c9a7fa9c016faa4b9 |
op_doi |
https://doi.org/10.1371/journal.pntd.0009104 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
15 |
container_issue |
2 |
container_start_page |
e0009104 |
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1766343436302024704 |