Vitamin D status contributes to the antimicrobial activity of macrophages against Mycobacterium leprae.
BACKGROUND:The immune system depends on effector pathways to eliminate invading pathogens from the host in vivo. Macrophages (MΦ) of the innate immune system are armed with vitamin D-dependent antimicrobial responses to kill intracellular microbes. However, how the physiological levels of vitamin D...
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ftdoajarticles:oai:doaj.org/article:db646e59030f48d098f3f8035be0704e 2023-05-15T15:10:37+02:00 Vitamin D status contributes to the antimicrobial activity of macrophages against Mycobacterium leprae. Elliot W Kim Rosane M B Teles Salem Haile Philip T Liu Robert L Modlin 2018-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006608 https://doaj.org/article/db646e59030f48d098f3f8035be0704e EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC6044553?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006608 https://doaj.org/article/db646e59030f48d098f3f8035be0704e PLoS Neglected Tropical Diseases, Vol 12, Iss 7, p e0006608 (2018) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2018 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006608 2022-12-31T16:28:27Z BACKGROUND:The immune system depends on effector pathways to eliminate invading pathogens from the host in vivo. Macrophages (MΦ) of the innate immune system are armed with vitamin D-dependent antimicrobial responses to kill intracellular microbes. However, how the physiological levels of vitamin D during MΦ differentiation affect phenotype and function is unknown. METHODOLOGY/PRINCIPAL:The human innate immune system consists of divergent MΦ subsets that serve distinct functions in vivo. Both IL-15 and IL-10 induce MΦ differentiation, but IL-15 induces primary human monocytes to differentiate into antimicrobial MΦ (IL-15 MΦ) that robustly express the vitamin D pathway. However, how vitamin D status alters IL-15 MΦ phenotype and function is unknown. In this study, we found that adding 25-hydroxyvitamin D3 (25D3) during the IL-15 induced differentiation of monocytes into MΦ increased the expression of the antimicrobial peptide cathelicidin, including both CAMP mRNA and the encoded protein cathelicidin in a dose-dependent manner. The presence of physiological levels of 25D during differentiation of IL-15 MΦ led to a significant vitamin D-dependent antimicrobial response against intracellular Mycobacterium leprae but did not change the phenotype or phagocytic function of these MΦ. These data suggest that activation of the vitamin D pathway during IL-15 MΦ differentiation augments the antimicrobial response against M. leprae infection. CONCLUSIONS/SIGNIFICANCE:Our data demonstrates that the presence of vitamin D during MΦ differentiation bestows the capacity to mount an antimicrobial response against M. leprae. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 12 7 e0006608 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Elliot W Kim Rosane M B Teles Salem Haile Philip T Liu Robert L Modlin Vitamin D status contributes to the antimicrobial activity of macrophages against Mycobacterium leprae. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:The immune system depends on effector pathways to eliminate invading pathogens from the host in vivo. Macrophages (MΦ) of the innate immune system are armed with vitamin D-dependent antimicrobial responses to kill intracellular microbes. However, how the physiological levels of vitamin D during MΦ differentiation affect phenotype and function is unknown. METHODOLOGY/PRINCIPAL:The human innate immune system consists of divergent MΦ subsets that serve distinct functions in vivo. Both IL-15 and IL-10 induce MΦ differentiation, but IL-15 induces primary human monocytes to differentiate into antimicrobial MΦ (IL-15 MΦ) that robustly express the vitamin D pathway. However, how vitamin D status alters IL-15 MΦ phenotype and function is unknown. In this study, we found that adding 25-hydroxyvitamin D3 (25D3) during the IL-15 induced differentiation of monocytes into MΦ increased the expression of the antimicrobial peptide cathelicidin, including both CAMP mRNA and the encoded protein cathelicidin in a dose-dependent manner. The presence of physiological levels of 25D during differentiation of IL-15 MΦ led to a significant vitamin D-dependent antimicrobial response against intracellular Mycobacterium leprae but did not change the phenotype or phagocytic function of these MΦ. These data suggest that activation of the vitamin D pathway during IL-15 MΦ differentiation augments the antimicrobial response against M. leprae infection. CONCLUSIONS/SIGNIFICANCE:Our data demonstrates that the presence of vitamin D during MΦ differentiation bestows the capacity to mount an antimicrobial response against M. leprae. |
format |
Article in Journal/Newspaper |
author |
Elliot W Kim Rosane M B Teles Salem Haile Philip T Liu Robert L Modlin |
author_facet |
Elliot W Kim Rosane M B Teles Salem Haile Philip T Liu Robert L Modlin |
author_sort |
Elliot W Kim |
title |
Vitamin D status contributes to the antimicrobial activity of macrophages against Mycobacterium leprae. |
title_short |
Vitamin D status contributes to the antimicrobial activity of macrophages against Mycobacterium leprae. |
title_full |
Vitamin D status contributes to the antimicrobial activity of macrophages against Mycobacterium leprae. |
title_fullStr |
Vitamin D status contributes to the antimicrobial activity of macrophages against Mycobacterium leprae. |
title_full_unstemmed |
Vitamin D status contributes to the antimicrobial activity of macrophages against Mycobacterium leprae. |
title_sort |
vitamin d status contributes to the antimicrobial activity of macrophages against mycobacterium leprae. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pntd.0006608 https://doaj.org/article/db646e59030f48d098f3f8035be0704e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 12, Iss 7, p e0006608 (2018) |
op_relation |
http://europepmc.org/articles/PMC6044553?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006608 https://doaj.org/article/db646e59030f48d098f3f8035be0704e |
op_doi |
https://doi.org/10.1371/journal.pntd.0006608 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
12 |
container_issue |
7 |
container_start_page |
e0006608 |
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1766341618422513664 |