Assessment of virus and Leptospira carriage in bats in France.
With over 1,400 species worldwide, bats represent the second largest order of mammals after rodents, and are known to host major zoonotic pathogens. Here, we estimate the presence of pathogens in autochthonous bat populations. First, we set out to check our samples for PCR amplification efficiency b...
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ftdoajarticles:oai:doaj.org/article:db592102ed21424eb174db1aa9bf2118 2023-12-03T10:20:04+01:00 Assessment of virus and Leptospira carriage in bats in France. Youssef Arnaout Evelyne Picard-Meyer Emmanuelle Robardet Julien Cappelle Florence Cliquet Frédéric Touzalin Giacomo Jimenez Zouheira Djelouadji 2023-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0292840 https://doaj.org/article/db592102ed21424eb174db1aa9bf2118 EN eng Public Library of Science (PLoS) https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0292840&type=printable https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0292840 https://doaj.org/article/db592102ed21424eb174db1aa9bf2118 PLoS ONE, Vol 18, Iss 10, p e0292840 (2023) Medicine R Science Q article 2023 ftdoajarticles https://doi.org/10.1371/journal.pone.0292840 2023-11-05T01:35:36Z With over 1,400 species worldwide, bats represent the second largest order of mammals after rodents, and are known to host major zoonotic pathogens. Here, we estimate the presence of pathogens in autochthonous bat populations. First, we set out to check our samples for PCR amplification efficiency by assessing the occurrence of inhibited PCR reactions from different types of bat samples with amplifying the housekeeping gene β-actin. Second, we investigated the presence of five targeted pathogens in a French bat population using PCR. We targeted viral RNA of Canine distemper virus, Alphacoronavirus, Lyssavirus, Rotavirus and bacterial Leptospira DNA. To do so, we screened for these viruses in bat faecal samples as well as in oropharyngeal swab samples. The presence of Leptospira was assessed in urine, kidney, lung and faecal samples. Results showed a frequency of inhibited reactions ranging from 5 to 60% of samples, varying according to the sample itself and also suspected to vary according to sampling method and the storage buffer solution used, demonstrating the importance of the sampling and storage on the probability of obtaining negative PCR results. For pathogen assessment, rotavirus and alphacoronavirus RNA were detected in Myotis myotis, Myotis daubentonii, Myotis emarginatus and Rhinolophus ferrumequinum bats. Rotaviruses were also detected in Barbastella barbastellus. The presence of alphacoronavirus also varied seasonally, with higher frequencies in late summer and October, suggesting that juveniles potentially play an important role in the dynamics of these viruses. Leptospira DNA was detected in M. myotis and M. daubentonii colonies. The 16S rRNA sequences obtained from Leptospira positive samples showed 100% genetic identity with L. borgpetersenii. Neither canine distemper virus nor lyssavirus RNA were detected in any of the tested samples. This study is the first to show the presence of Leptospira in autochthonous French bats in addition to coronavirus and rotavirus RNA previously reported in ... Article in Journal/Newspaper Barbastella barbastellus Directory of Open Access Journals: DOAJ Articles PLOS ONE 18 10 e0292840 |
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Medicine R Science Q Youssef Arnaout Evelyne Picard-Meyer Emmanuelle Robardet Julien Cappelle Florence Cliquet Frédéric Touzalin Giacomo Jimenez Zouheira Djelouadji Assessment of virus and Leptospira carriage in bats in France. |
topic_facet |
Medicine R Science Q |
description |
With over 1,400 species worldwide, bats represent the second largest order of mammals after rodents, and are known to host major zoonotic pathogens. Here, we estimate the presence of pathogens in autochthonous bat populations. First, we set out to check our samples for PCR amplification efficiency by assessing the occurrence of inhibited PCR reactions from different types of bat samples with amplifying the housekeeping gene β-actin. Second, we investigated the presence of five targeted pathogens in a French bat population using PCR. We targeted viral RNA of Canine distemper virus, Alphacoronavirus, Lyssavirus, Rotavirus and bacterial Leptospira DNA. To do so, we screened for these viruses in bat faecal samples as well as in oropharyngeal swab samples. The presence of Leptospira was assessed in urine, kidney, lung and faecal samples. Results showed a frequency of inhibited reactions ranging from 5 to 60% of samples, varying according to the sample itself and also suspected to vary according to sampling method and the storage buffer solution used, demonstrating the importance of the sampling and storage on the probability of obtaining negative PCR results. For pathogen assessment, rotavirus and alphacoronavirus RNA were detected in Myotis myotis, Myotis daubentonii, Myotis emarginatus and Rhinolophus ferrumequinum bats. Rotaviruses were also detected in Barbastella barbastellus. The presence of alphacoronavirus also varied seasonally, with higher frequencies in late summer and October, suggesting that juveniles potentially play an important role in the dynamics of these viruses. Leptospira DNA was detected in M. myotis and M. daubentonii colonies. The 16S rRNA sequences obtained from Leptospira positive samples showed 100% genetic identity with L. borgpetersenii. Neither canine distemper virus nor lyssavirus RNA were detected in any of the tested samples. This study is the first to show the presence of Leptospira in autochthonous French bats in addition to coronavirus and rotavirus RNA previously reported in ... |
format |
Article in Journal/Newspaper |
author |
Youssef Arnaout Evelyne Picard-Meyer Emmanuelle Robardet Julien Cappelle Florence Cliquet Frédéric Touzalin Giacomo Jimenez Zouheira Djelouadji |
author_facet |
Youssef Arnaout Evelyne Picard-Meyer Emmanuelle Robardet Julien Cappelle Florence Cliquet Frédéric Touzalin Giacomo Jimenez Zouheira Djelouadji |
author_sort |
Youssef Arnaout |
title |
Assessment of virus and Leptospira carriage in bats in France. |
title_short |
Assessment of virus and Leptospira carriage in bats in France. |
title_full |
Assessment of virus and Leptospira carriage in bats in France. |
title_fullStr |
Assessment of virus and Leptospira carriage in bats in France. |
title_full_unstemmed |
Assessment of virus and Leptospira carriage in bats in France. |
title_sort |
assessment of virus and leptospira carriage in bats in france. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2023 |
url |
https://doi.org/10.1371/journal.pone.0292840 https://doaj.org/article/db592102ed21424eb174db1aa9bf2118 |
genre |
Barbastella barbastellus |
genre_facet |
Barbastella barbastellus |
op_source |
PLoS ONE, Vol 18, Iss 10, p e0292840 (2023) |
op_relation |
https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0292840&type=printable https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0292840 https://doaj.org/article/db592102ed21424eb174db1aa9bf2118 |
op_doi |
https://doi.org/10.1371/journal.pone.0292840 |
container_title |
PLOS ONE |
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18 |
container_issue |
10 |
container_start_page |
e0292840 |
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