Identification and functional characterization of two executioner caspases in Crassostrea gigas.
Caspase-3 and caspase-7 are two key effector caspases that play important roles in apoptotic pathways that maintain normal tissue and organ development and homeostasis. However, little is known about the sequence, structure, activity, and function of effector caspases upon apoptosis in mollusks, esp...
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ftdoajarticles:oai:doaj.org/article:db45caa4add546a69970c4df0aa75fc7 2023-05-15T15:58:15+02:00 Identification and functional characterization of two executioner caspases in Crassostrea gigas. Tao Qu Baoyu Huang Linlin Zhang Li Li Fei Xu Wen Huang Chunyan Li Yishuai Du Guofan Zhang 2014-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0089040 https://doaj.org/article/db45caa4add546a69970c4df0aa75fc7 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3923871?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0089040 https://doaj.org/article/db45caa4add546a69970c4df0aa75fc7 PLoS ONE, Vol 9, Iss 2, p e89040 (2014) Medicine R Science Q article 2014 ftdoajarticles https://doi.org/10.1371/journal.pone.0089040 2022-12-31T01:50:13Z Caspase-3 and caspase-7 are two key effector caspases that play important roles in apoptotic pathways that maintain normal tissue and organ development and homeostasis. However, little is known about the sequence, structure, activity, and function of effector caspases upon apoptosis in mollusks, especially marine bivalves. In this study, we investigated the possible roles of two executioner caspases in the regulation of apoptosis in the Pacific oyster Crassostrea gigas. A full-length caspase-3-like gene named Cgcaspase-3 was cloned from C.gigas cDNA, encoding a predicted protein containing caspase family p20 and p10 domain profiles and a conserved caspase active site motif. Phylogenetic analysis demonstrated that both Cgcaspase-3 and Cgcaspase-1 may function as effector caspases clustered in the invertebrate branch. Although the sequence identities between the two caspases was low, both enzymes possessed executioner caspase activity and were capable of inducing cell death. These results suggested that Cgcaspase-3 and Cgcaspase-1 were two effector caspases in C. gigas. We also observed that nucleus-localized Cgcaspase-3, may function as a caspase-3-like protein and cytoplasm-localized Cgcaspase-1 may function as a caspase-7-like protein. Both Cgcaspase-3 and Cgcaspase-1 mRNA expression increased after larvae settled on the substratum, suggesting that both caspases acted in several tissues or organs that degenerated after oyster larvae settlement. The highest caspase expression levels were observed in the gills indicating that both effector caspases were likely involved in immune or metabolic processes in C. gigas. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific PLoS ONE 9 2 e89040 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
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topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Tao Qu Baoyu Huang Linlin Zhang Li Li Fei Xu Wen Huang Chunyan Li Yishuai Du Guofan Zhang Identification and functional characterization of two executioner caspases in Crassostrea gigas. |
topic_facet |
Medicine R Science Q |
description |
Caspase-3 and caspase-7 are two key effector caspases that play important roles in apoptotic pathways that maintain normal tissue and organ development and homeostasis. However, little is known about the sequence, structure, activity, and function of effector caspases upon apoptosis in mollusks, especially marine bivalves. In this study, we investigated the possible roles of two executioner caspases in the regulation of apoptosis in the Pacific oyster Crassostrea gigas. A full-length caspase-3-like gene named Cgcaspase-3 was cloned from C.gigas cDNA, encoding a predicted protein containing caspase family p20 and p10 domain profiles and a conserved caspase active site motif. Phylogenetic analysis demonstrated that both Cgcaspase-3 and Cgcaspase-1 may function as effector caspases clustered in the invertebrate branch. Although the sequence identities between the two caspases was low, both enzymes possessed executioner caspase activity and were capable of inducing cell death. These results suggested that Cgcaspase-3 and Cgcaspase-1 were two effector caspases in C. gigas. We also observed that nucleus-localized Cgcaspase-3, may function as a caspase-3-like protein and cytoplasm-localized Cgcaspase-1 may function as a caspase-7-like protein. Both Cgcaspase-3 and Cgcaspase-1 mRNA expression increased after larvae settled on the substratum, suggesting that both caspases acted in several tissues or organs that degenerated after oyster larvae settlement. The highest caspase expression levels were observed in the gills indicating that both effector caspases were likely involved in immune or metabolic processes in C. gigas. |
format |
Article in Journal/Newspaper |
author |
Tao Qu Baoyu Huang Linlin Zhang Li Li Fei Xu Wen Huang Chunyan Li Yishuai Du Guofan Zhang |
author_facet |
Tao Qu Baoyu Huang Linlin Zhang Li Li Fei Xu Wen Huang Chunyan Li Yishuai Du Guofan Zhang |
author_sort |
Tao Qu |
title |
Identification and functional characterization of two executioner caspases in Crassostrea gigas. |
title_short |
Identification and functional characterization of two executioner caspases in Crassostrea gigas. |
title_full |
Identification and functional characterization of two executioner caspases in Crassostrea gigas. |
title_fullStr |
Identification and functional characterization of two executioner caspases in Crassostrea gigas. |
title_full_unstemmed |
Identification and functional characterization of two executioner caspases in Crassostrea gigas. |
title_sort |
identification and functional characterization of two executioner caspases in crassostrea gigas. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pone.0089040 https://doaj.org/article/db45caa4add546a69970c4df0aa75fc7 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
PLoS ONE, Vol 9, Iss 2, p e89040 (2014) |
op_relation |
http://europepmc.org/articles/PMC3923871?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0089040 https://doaj.org/article/db45caa4add546a69970c4df0aa75fc7 |
op_doi |
https://doi.org/10.1371/journal.pone.0089040 |
container_title |
PLoS ONE |
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9 |
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2 |
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e89040 |
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1766393977859211264 |