Neuritogenic effect of sea cucumber glucocerebrosides on NGF-induced PC12 cells via activation of the TrkA/CREB/BDNF signalling pathway
Sea cucumber glucocerebrosides (SCGs), an important sphingolipid, have extensive functions in inhibiting the growth of cancer cells, but their potential to promote neuronal outgrowth hasn’t been well studied. Herein, we evaluated the neuritogenic effects of three Cucumaria frondosa SCGs series (SCG-...
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ftdoajarticles:oai:doaj.org/article:db314bde19e4483eb402f9668d7965cb 2023-05-15T15:59:38+02:00 Neuritogenic effect of sea cucumber glucocerebrosides on NGF-induced PC12 cells via activation of the TrkA/CREB/BDNF signalling pathway Xiaoxu Wang Peixu Cong Yanjun Liu Suyuan Tao Qinsheng Chen Jingfeng Wang Jie Xu Changhu Xue 2018-07-01T00:00:00Z https://doi.org/10.1016/j.jff.2018.04.035 https://doaj.org/article/db314bde19e4483eb402f9668d7965cb EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S1756464618301725 https://doaj.org/toc/1756-4646 1756-4646 doi:10.1016/j.jff.2018.04.035 https://doaj.org/article/db314bde19e4483eb402f9668d7965cb Journal of Functional Foods, Vol 46, Iss , Pp 175-184 (2018) PC12 cells Sea cucumber cerebrosides Neuritogenic effect TrkA CREB BDNF Nutrition. Foods and food supply TX341-641 article 2018 ftdoajarticles https://doi.org/10.1016/j.jff.2018.04.035 2022-12-31T06:52:49Z Sea cucumber glucocerebrosides (SCGs), an important sphingolipid, have extensive functions in inhibiting the growth of cancer cells, but their potential to promote neuronal outgrowth hasn’t been well studied. Herein, we evaluated the neuritogenic effects of three Cucumaria frondosa SCGs series (SCG-1, SCG-2, and SCG-3) in NGF-induced PC12 cells. These SCGs exhibited significant neuritogenic effects in a dose-dependent and structure-selective manner, which increased the ratio of neurite-bearing cells and expression of axonal (GAP-43) and synaptic (synaptophysin) proteins. Mechanistic studies suggested that SCGs reinforced the NGF-induced phosphorylation of TrkA, coupled with ERK1/2 activation, then triggered the activation of CREB, finally resulted in a marked increase of BDNF expression. Furthermore, the neuritogenic effects of SCGs could be suppressed when accompanied by the additions of TrkA inhibitor GW441765 or MEK inhibitor U0126. In conclusion, SCGs possess a marked neuritogenic effect that promotes the signalling downstream from TrkA, likely via the MEK/ERK1/2/CREB/BDNF pathway. Article in Journal/Newspaper Cucumaria frondosa Directory of Open Access Journals: DOAJ Articles Mek ENVELOPE(7.086,7.086,62.735,62.735) Journal of Functional Foods 46 175 184 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
PC12 cells Sea cucumber cerebrosides Neuritogenic effect TrkA CREB BDNF Nutrition. Foods and food supply TX341-641 |
spellingShingle |
PC12 cells Sea cucumber cerebrosides Neuritogenic effect TrkA CREB BDNF Nutrition. Foods and food supply TX341-641 Xiaoxu Wang Peixu Cong Yanjun Liu Suyuan Tao Qinsheng Chen Jingfeng Wang Jie Xu Changhu Xue Neuritogenic effect of sea cucumber glucocerebrosides on NGF-induced PC12 cells via activation of the TrkA/CREB/BDNF signalling pathway |
topic_facet |
PC12 cells Sea cucumber cerebrosides Neuritogenic effect TrkA CREB BDNF Nutrition. Foods and food supply TX341-641 |
description |
Sea cucumber glucocerebrosides (SCGs), an important sphingolipid, have extensive functions in inhibiting the growth of cancer cells, but their potential to promote neuronal outgrowth hasn’t been well studied. Herein, we evaluated the neuritogenic effects of three Cucumaria frondosa SCGs series (SCG-1, SCG-2, and SCG-3) in NGF-induced PC12 cells. These SCGs exhibited significant neuritogenic effects in a dose-dependent and structure-selective manner, which increased the ratio of neurite-bearing cells and expression of axonal (GAP-43) and synaptic (synaptophysin) proteins. Mechanistic studies suggested that SCGs reinforced the NGF-induced phosphorylation of TrkA, coupled with ERK1/2 activation, then triggered the activation of CREB, finally resulted in a marked increase of BDNF expression. Furthermore, the neuritogenic effects of SCGs could be suppressed when accompanied by the additions of TrkA inhibitor GW441765 or MEK inhibitor U0126. In conclusion, SCGs possess a marked neuritogenic effect that promotes the signalling downstream from TrkA, likely via the MEK/ERK1/2/CREB/BDNF pathway. |
format |
Article in Journal/Newspaper |
author |
Xiaoxu Wang Peixu Cong Yanjun Liu Suyuan Tao Qinsheng Chen Jingfeng Wang Jie Xu Changhu Xue |
author_facet |
Xiaoxu Wang Peixu Cong Yanjun Liu Suyuan Tao Qinsheng Chen Jingfeng Wang Jie Xu Changhu Xue |
author_sort |
Xiaoxu Wang |
title |
Neuritogenic effect of sea cucumber glucocerebrosides on NGF-induced PC12 cells via activation of the TrkA/CREB/BDNF signalling pathway |
title_short |
Neuritogenic effect of sea cucumber glucocerebrosides on NGF-induced PC12 cells via activation of the TrkA/CREB/BDNF signalling pathway |
title_full |
Neuritogenic effect of sea cucumber glucocerebrosides on NGF-induced PC12 cells via activation of the TrkA/CREB/BDNF signalling pathway |
title_fullStr |
Neuritogenic effect of sea cucumber glucocerebrosides on NGF-induced PC12 cells via activation of the TrkA/CREB/BDNF signalling pathway |
title_full_unstemmed |
Neuritogenic effect of sea cucumber glucocerebrosides on NGF-induced PC12 cells via activation of the TrkA/CREB/BDNF signalling pathway |
title_sort |
neuritogenic effect of sea cucumber glucocerebrosides on ngf-induced pc12 cells via activation of the trka/creb/bdnf signalling pathway |
publisher |
Elsevier |
publishDate |
2018 |
url |
https://doi.org/10.1016/j.jff.2018.04.035 https://doaj.org/article/db314bde19e4483eb402f9668d7965cb |
long_lat |
ENVELOPE(7.086,7.086,62.735,62.735) |
geographic |
Mek |
geographic_facet |
Mek |
genre |
Cucumaria frondosa |
genre_facet |
Cucumaria frondosa |
op_source |
Journal of Functional Foods, Vol 46, Iss , Pp 175-184 (2018) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S1756464618301725 https://doaj.org/toc/1756-4646 1756-4646 doi:10.1016/j.jff.2018.04.035 https://doaj.org/article/db314bde19e4483eb402f9668d7965cb |
op_doi |
https://doi.org/10.1016/j.jff.2018.04.035 |
container_title |
Journal of Functional Foods |
container_volume |
46 |
container_start_page |
175 |
op_container_end_page |
184 |
_version_ |
1766395568427368448 |