Prevalence of resistance phenotypes in Staphylococcus aureus and coagulase-negative isolates of venous ulcers of primary healthcare patients
INTRODUCTION: In venous ulcers, the presence of Staphylococcus aureus and coagulase-negative staphylococcus resistance phenotypes can aggravate and limit the choices for treatment. METHODS: Staphylococcus isolated from 69 patients (98 ulcers) between October of 2009 and October of 2010 were tested....
Published in: | Revista da Sociedade Brasileira de Medicina Tropical |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Sociedade Brasileira de Medicina Tropical (SBMT)
2012
|
Subjects: | |
Online Access: | https://doi.org/10.1590/S0037-86822012000600012 https://doaj.org/article/db1e1c085d734f4da6b5e40f09b15482 |
id |
ftdoajarticles:oai:doaj.org/article:db1e1c085d734f4da6b5e40f09b15482 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:db1e1c085d734f4da6b5e40f09b15482 2023-05-15T15:12:52+02:00 Prevalence of resistance phenotypes in Staphylococcus aureus and coagulase-negative isolates of venous ulcers of primary healthcare patients Marlene Andrade Martins Silvana de Lima Vieira dos Santos Lara Stefânia Netto de Oliveira Leão Nayara Portilho Araújo Maria Márcia Bachion 2012-12-01T00:00:00Z https://doi.org/10.1590/S0037-86822012000600012 https://doaj.org/article/db1e1c085d734f4da6b5e40f09b15482 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000600012&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/S0037-86822012000600012 https://doaj.org/article/db1e1c085d734f4da6b5e40f09b15482 Revista da Sociedade Brasileira de Medicina Tropical, Vol 45, Iss 6, Pp 717-722 (2012) Staphylococcus aureus resistente à meticilina Resistência bacteriana a fármacos Úlcera varicosa Atenção primária à saúde Arctic medicine. Tropical medicine RC955-962 article 2012 ftdoajarticles https://doi.org/10.1590/S0037-86822012000600012 2022-12-31T02:53:15Z INTRODUCTION: In venous ulcers, the presence of Staphylococcus aureus and coagulase-negative staphylococcus resistance phenotypes can aggravate and limit the choices for treatment. METHODS: Staphylococcus isolated from 69 patients (98 ulcers) between October of 2009 and October of 2010 were tested. The macrolide, lincosamide, streptogramin B (MLS B) group resistance phenotype detection was performed using the D-test. Isolates resistant to cefoxitin and/or oxacillin (disk-diffusion) were subjected to the confirmatory test to detect minimum inhibitory concentration (MIC), using oxacillin strips (E-test®). RESULTS: The prevalence of S. aureus was 83%, and 15% of coagulase-negative staphylococcus (CoNS). In addition were detected 28% of methicillin-resistant Staphylococcus aureus (MRSA) and 47% of methicillin-resistant coagulase-negative staphylococcus (MRCoNS). Among the S. aureus, 69.6% were resistant to erythromycin, 69.6% to clindamycin, 69.6% to gentamicin, and 100% to ciprofloxacin. Considering the MRSA, 74% were highly resistant to oxacillin, MIC ≥ 256µg/mL, and the MLS Bc constitutive resistance predominated in 65.2%. Among the 20 isolates sensitive to clindamycin, 12 presented an inducible MLS B phenotype. Of the MRCoNS, 71.4%were resistant to erythromycin, ciprofloxacin and gentamicin. Considering the isolates positive for β-lactamases, the MIC breakpoint was between 0.5 and 2µg/mL. CONCLUSIONS: The results point to a high occurrence of multi-drug resistant bacteria in venous ulcers in primary healthcare patients, thus evidencing the need for preventive measures to avoid outbreaks caused by multi-drug resistant pathogens, and the importance of healthcare professionals being able to identifying colonized versus infected venous ulcers as an essential criteria to implementing systemic antibacterial therapy. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 45 6 717 722 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Staphylococcus aureus resistente à meticilina Resistência bacteriana a fármacos Úlcera varicosa Atenção primária à saúde Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Staphylococcus aureus resistente à meticilina Resistência bacteriana a fármacos Úlcera varicosa Atenção primária à saúde Arctic medicine. Tropical medicine RC955-962 Marlene Andrade Martins Silvana de Lima Vieira dos Santos Lara Stefânia Netto de Oliveira Leão Nayara Portilho Araújo Maria Márcia Bachion Prevalence of resistance phenotypes in Staphylococcus aureus and coagulase-negative isolates of venous ulcers of primary healthcare patients |
topic_facet |
Staphylococcus aureus resistente à meticilina Resistência bacteriana a fármacos Úlcera varicosa Atenção primária à saúde Arctic medicine. Tropical medicine RC955-962 |
description |
INTRODUCTION: In venous ulcers, the presence of Staphylococcus aureus and coagulase-negative staphylococcus resistance phenotypes can aggravate and limit the choices for treatment. METHODS: Staphylococcus isolated from 69 patients (98 ulcers) between October of 2009 and October of 2010 were tested. The macrolide, lincosamide, streptogramin B (MLS B) group resistance phenotype detection was performed using the D-test. Isolates resistant to cefoxitin and/or oxacillin (disk-diffusion) were subjected to the confirmatory test to detect minimum inhibitory concentration (MIC), using oxacillin strips (E-test®). RESULTS: The prevalence of S. aureus was 83%, and 15% of coagulase-negative staphylococcus (CoNS). In addition were detected 28% of methicillin-resistant Staphylococcus aureus (MRSA) and 47% of methicillin-resistant coagulase-negative staphylococcus (MRCoNS). Among the S. aureus, 69.6% were resistant to erythromycin, 69.6% to clindamycin, 69.6% to gentamicin, and 100% to ciprofloxacin. Considering the MRSA, 74% were highly resistant to oxacillin, MIC ≥ 256µg/mL, and the MLS Bc constitutive resistance predominated in 65.2%. Among the 20 isolates sensitive to clindamycin, 12 presented an inducible MLS B phenotype. Of the MRCoNS, 71.4%were resistant to erythromycin, ciprofloxacin and gentamicin. Considering the isolates positive for β-lactamases, the MIC breakpoint was between 0.5 and 2µg/mL. CONCLUSIONS: The results point to a high occurrence of multi-drug resistant bacteria in venous ulcers in primary healthcare patients, thus evidencing the need for preventive measures to avoid outbreaks caused by multi-drug resistant pathogens, and the importance of healthcare professionals being able to identifying colonized versus infected venous ulcers as an essential criteria to implementing systemic antibacterial therapy. |
format |
Article in Journal/Newspaper |
author |
Marlene Andrade Martins Silvana de Lima Vieira dos Santos Lara Stefânia Netto de Oliveira Leão Nayara Portilho Araújo Maria Márcia Bachion |
author_facet |
Marlene Andrade Martins Silvana de Lima Vieira dos Santos Lara Stefânia Netto de Oliveira Leão Nayara Portilho Araújo Maria Márcia Bachion |
author_sort |
Marlene Andrade Martins |
title |
Prevalence of resistance phenotypes in Staphylococcus aureus and coagulase-negative isolates of venous ulcers of primary healthcare patients |
title_short |
Prevalence of resistance phenotypes in Staphylococcus aureus and coagulase-negative isolates of venous ulcers of primary healthcare patients |
title_full |
Prevalence of resistance phenotypes in Staphylococcus aureus and coagulase-negative isolates of venous ulcers of primary healthcare patients |
title_fullStr |
Prevalence of resistance phenotypes in Staphylococcus aureus and coagulase-negative isolates of venous ulcers of primary healthcare patients |
title_full_unstemmed |
Prevalence of resistance phenotypes in Staphylococcus aureus and coagulase-negative isolates of venous ulcers of primary healthcare patients |
title_sort |
prevalence of resistance phenotypes in staphylococcus aureus and coagulase-negative isolates of venous ulcers of primary healthcare patients |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2012 |
url |
https://doi.org/10.1590/S0037-86822012000600012 https://doaj.org/article/db1e1c085d734f4da6b5e40f09b15482 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 45, Iss 6, Pp 717-722 (2012) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000600012&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/S0037-86822012000600012 https://doaj.org/article/db1e1c085d734f4da6b5e40f09b15482 |
op_doi |
https://doi.org/10.1590/S0037-86822012000600012 |
container_title |
Revista da Sociedade Brasileira de Medicina Tropical |
container_volume |
45 |
container_issue |
6 |
container_start_page |
717 |
op_container_end_page |
722 |
_version_ |
1766343496372846592 |