S100, CD68, and MHC class II molecule expression in cervical high- and low-grade HPV-induced lesions
INTRODUCTION: Some human papillomavirus (HPV) types are involved in malignant processes in the cervical epithelium, with 99% of cases attributed to oncogenic HPV infection. This study aimed to detect S100, CD68, and major histocompatibility complex class II (MHC-II) molecules in cervical uterine epi...
Published in: | Revista da Sociedade Brasileira de Medicina Tropical |
---|---|
Main Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Sociedade Brasileira de Medicina Tropical (SBMT)
2012
|
Subjects: | |
Online Access: | https://doi.org/10.1590/S0037-86822012000100002 https://doaj.org/article/dae18cedf4dd4bb6afed381e9006356e |
id |
ftdoajarticles:oai:doaj.org/article:dae18cedf4dd4bb6afed381e9006356e |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:dae18cedf4dd4bb6afed381e9006356e 2023-05-15T15:09:26+02:00 S100, CD68, and MHC class II molecule expression in cervical high- and low-grade HPV-induced lesions Fernanda Cassandri Inês Aparecida Tozetti Carlos Eurico dos Santos Fernandes Flávia Gatto de Almeida Gustavo Ribeiro Falcão Ilzia Doraci Lins Scapulatempo Thiago Theodoro Martins Prata Cacilda Tezelli Junqueira Padovani Daniella Borges Alves Alda Teixeira Ferreira Maria Auxiliadora Gomes Sandim Abdo 2012-02-01T00:00:00Z https://doi.org/10.1590/S0037-86822012000100002 https://doaj.org/article/dae18cedf4dd4bb6afed381e9006356e EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000100002&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/S0037-86822012000100002 https://doaj.org/article/dae18cedf4dd4bb6afed381e9006356e Revista da Sociedade Brasileira de Medicina Tropical, Vol 45, Iss 1, Pp 3-8 (2012) Papilomavírus humano Resposta immune Imunohistoquímica Arctic medicine. Tropical medicine RC955-962 article 2012 ftdoajarticles https://doi.org/10.1590/S0037-86822012000100002 2022-12-31T02:52:53Z INTRODUCTION: Some human papillomavirus (HPV) types are involved in malignant processes in the cervical epithelium, with 99% of cases attributed to oncogenic HPV infection. This study aimed to detect S100, CD68, and major histocompatibility complex class II (MHC-II) molecules in cervical uterine epithelial samples in patients with high- and low-grade lesions induced by HPV. METHODS: Fifty-eight samples from patients who were confirmed positive or negative for high-risk oncogenic HPV DNA, had histopathological diagnosis of cervical intraepithelial neoplasia (CIN) of grades I, II, or III, or were negative for intraepithelial lesion or malignancy were subjected to immunohistochemistry reaction to S100 protein, CD68, and MHC-II (HLA-DR alpha chain). RESULTS: The presence of MHC-II predominated in samples exhibiting histopathological alterations (p < 0.05). S100 detection was more numerous in carcinoma samples (CIN III) (75%). Presence of this protein correlated significantly (p < 0.05) with histopathological findings and viral load. CONCLUSIONS: A small expression of CD68 was observed, which may be explained by the observation in our study having been made on random microscopic fields and not on specific areas. The findings, such as the presence of S100 protein and MHC-II expression in samples with histological alterations, could suggest that the immune system fails to control HPV replication at the early stages of infection. Further studies with larger prospective data are necessary to confirm this result. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 45 1 3 8 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Papilomavírus humano Resposta immune Imunohistoquímica Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Papilomavírus humano Resposta immune Imunohistoquímica Arctic medicine. Tropical medicine RC955-962 Fernanda Cassandri Inês Aparecida Tozetti Carlos Eurico dos Santos Fernandes Flávia Gatto de Almeida Gustavo Ribeiro Falcão Ilzia Doraci Lins Scapulatempo Thiago Theodoro Martins Prata Cacilda Tezelli Junqueira Padovani Daniella Borges Alves Alda Teixeira Ferreira Maria Auxiliadora Gomes Sandim Abdo S100, CD68, and MHC class II molecule expression in cervical high- and low-grade HPV-induced lesions |
topic_facet |
Papilomavírus humano Resposta immune Imunohistoquímica Arctic medicine. Tropical medicine RC955-962 |
description |
INTRODUCTION: Some human papillomavirus (HPV) types are involved in malignant processes in the cervical epithelium, with 99% of cases attributed to oncogenic HPV infection. This study aimed to detect S100, CD68, and major histocompatibility complex class II (MHC-II) molecules in cervical uterine epithelial samples in patients with high- and low-grade lesions induced by HPV. METHODS: Fifty-eight samples from patients who were confirmed positive or negative for high-risk oncogenic HPV DNA, had histopathological diagnosis of cervical intraepithelial neoplasia (CIN) of grades I, II, or III, or were negative for intraepithelial lesion or malignancy were subjected to immunohistochemistry reaction to S100 protein, CD68, and MHC-II (HLA-DR alpha chain). RESULTS: The presence of MHC-II predominated in samples exhibiting histopathological alterations (p < 0.05). S100 detection was more numerous in carcinoma samples (CIN III) (75%). Presence of this protein correlated significantly (p < 0.05) with histopathological findings and viral load. CONCLUSIONS: A small expression of CD68 was observed, which may be explained by the observation in our study having been made on random microscopic fields and not on specific areas. The findings, such as the presence of S100 protein and MHC-II expression in samples with histological alterations, could suggest that the immune system fails to control HPV replication at the early stages of infection. Further studies with larger prospective data are necessary to confirm this result. |
format |
Article in Journal/Newspaper |
author |
Fernanda Cassandri Inês Aparecida Tozetti Carlos Eurico dos Santos Fernandes Flávia Gatto de Almeida Gustavo Ribeiro Falcão Ilzia Doraci Lins Scapulatempo Thiago Theodoro Martins Prata Cacilda Tezelli Junqueira Padovani Daniella Borges Alves Alda Teixeira Ferreira Maria Auxiliadora Gomes Sandim Abdo |
author_facet |
Fernanda Cassandri Inês Aparecida Tozetti Carlos Eurico dos Santos Fernandes Flávia Gatto de Almeida Gustavo Ribeiro Falcão Ilzia Doraci Lins Scapulatempo Thiago Theodoro Martins Prata Cacilda Tezelli Junqueira Padovani Daniella Borges Alves Alda Teixeira Ferreira Maria Auxiliadora Gomes Sandim Abdo |
author_sort |
Fernanda Cassandri |
title |
S100, CD68, and MHC class II molecule expression in cervical high- and low-grade HPV-induced lesions |
title_short |
S100, CD68, and MHC class II molecule expression in cervical high- and low-grade HPV-induced lesions |
title_full |
S100, CD68, and MHC class II molecule expression in cervical high- and low-grade HPV-induced lesions |
title_fullStr |
S100, CD68, and MHC class II molecule expression in cervical high- and low-grade HPV-induced lesions |
title_full_unstemmed |
S100, CD68, and MHC class II molecule expression in cervical high- and low-grade HPV-induced lesions |
title_sort |
s100, cd68, and mhc class ii molecule expression in cervical high- and low-grade hpv-induced lesions |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2012 |
url |
https://doi.org/10.1590/S0037-86822012000100002 https://doaj.org/article/dae18cedf4dd4bb6afed381e9006356e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 45, Iss 1, Pp 3-8 (2012) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000100002&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/S0037-86822012000100002 https://doaj.org/article/dae18cedf4dd4bb6afed381e9006356e |
op_doi |
https://doi.org/10.1590/S0037-86822012000100002 |
container_title |
Revista da Sociedade Brasileira de Medicina Tropical |
container_volume |
45 |
container_issue |
1 |
container_start_page |
3 |
op_container_end_page |
8 |
_version_ |
1766340631915921408 |