First Description of a New Disease in Rainbow Trout (Oncorhynchus mykiss (Walbaum)) Similar to Heart and Skeletal Muscle Inflammation (HSMI) and Detection of a Gene Sequence Related to Piscine Orthoreovirus (PRV).
In fall 2013, anorexia, lethargy and mortalities up to 10-12,000 dead fish per week were observed in rainbow trout Oncorhynchus mykiss in three fresh water hatcheries (salinity 0-1 ‰) on the west coast of Norway. The fish (25-100 g) showed signs of circulatory failure with haemorrhages, ascites and...
Published in: | PLOS ONE |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2015
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pone.0131638 https://doaj.org/article/dac31c4aebca47c9a22cfde90c6268de |
id |
ftdoajarticles:oai:doaj.org/article:dac31c4aebca47c9a22cfde90c6268de |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:dac31c4aebca47c9a22cfde90c6268de 2023-05-15T15:32:47+02:00 First Description of a New Disease in Rainbow Trout (Oncorhynchus mykiss (Walbaum)) Similar to Heart and Skeletal Muscle Inflammation (HSMI) and Detection of a Gene Sequence Related to Piscine Orthoreovirus (PRV). Anne Berit Olsen Monika Hjortaas Torstein Tengs Hege Hellberg Renate Johansen 2015-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0131638 https://doaj.org/article/dac31c4aebca47c9a22cfde90c6268de EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4503464?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0131638 https://doaj.org/article/dac31c4aebca47c9a22cfde90c6268de PLoS ONE, Vol 10, Iss 7, p e0131638 (2015) Medicine R Science Q article 2015 ftdoajarticles https://doi.org/10.1371/journal.pone.0131638 2022-12-31T12:24:22Z In fall 2013, anorexia, lethargy and mortalities up to 10-12,000 dead fish per week were observed in rainbow trout Oncorhynchus mykiss in three fresh water hatcheries (salinity 0-1 ‰) on the west coast of Norway. The fish (25-100 g) showed signs of circulatory failure with haemorrhages, ascites and anaemia. The histopathological findings comprised inflammation of the heart and red muscle and liver necrosis. The affected fish had a common origin. Disease and mortalities were also observed up to four months after sea water transfer. Microbiological examination did not reveal presence of any known pathogens. Based on histopathological similarities to heart and skeletal inflammation (HSMI) in Atlantic salmon, associated with piscine orthoreovirus (PRV), extended investigations to detect a virus within the family Reoviridae were conducted. By the use of primer sets targeting the PRV genome, a sequence with 85% identity to a part of segment S1 of PRV was obtained. Further analysis showed that the virus sequence could only be aligned with PRV and no other reoviruses both on amino acid and nucleotide level. Two PCR assays were developed for specific detection of the virus. High amounts of the virus were detected in diseased fish at all affected farms and low amounts were detected in low prevalence at the broodfish farms. Further investigations are needed to determine if the virus is associated with the new disease in rainbow trout and to further characterize the virus with respect to classification, relationship with PRV, virulence, pathology and epidemiology. Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles Norway PLOS ONE 10 7 e0131638 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Anne Berit Olsen Monika Hjortaas Torstein Tengs Hege Hellberg Renate Johansen First Description of a New Disease in Rainbow Trout (Oncorhynchus mykiss (Walbaum)) Similar to Heart and Skeletal Muscle Inflammation (HSMI) and Detection of a Gene Sequence Related to Piscine Orthoreovirus (PRV). |
topic_facet |
Medicine R Science Q |
description |
In fall 2013, anorexia, lethargy and mortalities up to 10-12,000 dead fish per week were observed in rainbow trout Oncorhynchus mykiss in three fresh water hatcheries (salinity 0-1 ‰) on the west coast of Norway. The fish (25-100 g) showed signs of circulatory failure with haemorrhages, ascites and anaemia. The histopathological findings comprised inflammation of the heart and red muscle and liver necrosis. The affected fish had a common origin. Disease and mortalities were also observed up to four months after sea water transfer. Microbiological examination did not reveal presence of any known pathogens. Based on histopathological similarities to heart and skeletal inflammation (HSMI) in Atlantic salmon, associated with piscine orthoreovirus (PRV), extended investigations to detect a virus within the family Reoviridae were conducted. By the use of primer sets targeting the PRV genome, a sequence with 85% identity to a part of segment S1 of PRV was obtained. Further analysis showed that the virus sequence could only be aligned with PRV and no other reoviruses both on amino acid and nucleotide level. Two PCR assays were developed for specific detection of the virus. High amounts of the virus were detected in diseased fish at all affected farms and low amounts were detected in low prevalence at the broodfish farms. Further investigations are needed to determine if the virus is associated with the new disease in rainbow trout and to further characterize the virus with respect to classification, relationship with PRV, virulence, pathology and epidemiology. |
format |
Article in Journal/Newspaper |
author |
Anne Berit Olsen Monika Hjortaas Torstein Tengs Hege Hellberg Renate Johansen |
author_facet |
Anne Berit Olsen Monika Hjortaas Torstein Tengs Hege Hellberg Renate Johansen |
author_sort |
Anne Berit Olsen |
title |
First Description of a New Disease in Rainbow Trout (Oncorhynchus mykiss (Walbaum)) Similar to Heart and Skeletal Muscle Inflammation (HSMI) and Detection of a Gene Sequence Related to Piscine Orthoreovirus (PRV). |
title_short |
First Description of a New Disease in Rainbow Trout (Oncorhynchus mykiss (Walbaum)) Similar to Heart and Skeletal Muscle Inflammation (HSMI) and Detection of a Gene Sequence Related to Piscine Orthoreovirus (PRV). |
title_full |
First Description of a New Disease in Rainbow Trout (Oncorhynchus mykiss (Walbaum)) Similar to Heart and Skeletal Muscle Inflammation (HSMI) and Detection of a Gene Sequence Related to Piscine Orthoreovirus (PRV). |
title_fullStr |
First Description of a New Disease in Rainbow Trout (Oncorhynchus mykiss (Walbaum)) Similar to Heart and Skeletal Muscle Inflammation (HSMI) and Detection of a Gene Sequence Related to Piscine Orthoreovirus (PRV). |
title_full_unstemmed |
First Description of a New Disease in Rainbow Trout (Oncorhynchus mykiss (Walbaum)) Similar to Heart and Skeletal Muscle Inflammation (HSMI) and Detection of a Gene Sequence Related to Piscine Orthoreovirus (PRV). |
title_sort |
first description of a new disease in rainbow trout (oncorhynchus mykiss (walbaum)) similar to heart and skeletal muscle inflammation (hsmi) and detection of a gene sequence related to piscine orthoreovirus (prv). |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pone.0131638 https://doaj.org/article/dac31c4aebca47c9a22cfde90c6268de |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
PLoS ONE, Vol 10, Iss 7, p e0131638 (2015) |
op_relation |
http://europepmc.org/articles/PMC4503464?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0131638 https://doaj.org/article/dac31c4aebca47c9a22cfde90c6268de |
op_doi |
https://doi.org/10.1371/journal.pone.0131638 |
container_title |
PLOS ONE |
container_volume |
10 |
container_issue |
7 |
container_start_page |
e0131638 |
_version_ |
1766363272484749312 |