Bacteria of the genus Pseudomonas isolated from Antarctic substrates
The study’s primary purposes were establishing the number of microorganisms that exhibit hydrolytic activity in Antarctic soil and mosses samples, isolation of metal-resistant strains of bacteria, and description of their physiological and biochemical properties. Samples collected during the XXIII U...
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State Institution National Antarctic Scientific Center
2021
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ftdoajarticles:oai:doaj.org/article:da94d2c8d1114195844aaa4bc9c0079c 2023-05-15T13:56:11+02:00 Bacteria of the genus Pseudomonas isolated from Antarctic substrates S. Hnatush S. Komplikevych O. Maslovska O. Moroz T. Peretyatko А. Dzhulai T. Krasnozhon 2021-12-01T00:00:00Z https://doi.org/10.33275/1727-7485.2.2021.678 https://doaj.org/article/da94d2c8d1114195844aaa4bc9c0079c EN UK eng ukr State Institution National Antarctic Scientific Center http://uaj.uac.gov.ua/index.php/uaj/article/view/678 https://doaj.org/toc/1727-7485 https://doaj.org/toc/2415-3087 1727-7485 2415-3087 doi:10.33275/1727-7485.2.2021.678 https://doaj.org/article/da94d2c8d1114195844aaa4bc9c0079c Український антарктичний журнал, Iss 2, Pp 58-75 (2021) enzymatic activity exopolysaccharides metal resistance phylogenetic reconstruction pseudomonas arsenicoxidans pseudomonas yamanorum Meteorology. Climatology QC851-999 Geophysics. Cosmic physics QC801-809 article 2021 ftdoajarticles https://doi.org/10.33275/1727-7485.2.2021.678 2022-12-31T15:08:42Z The study’s primary purposes were establishing the number of microorganisms that exhibit hydrolytic activity in Antarctic soil and mosses samples, isolation of metal-resistant strains of bacteria, and description of their physiological and biochemical properties. Samples collected during the XXIII Ukrainian Antarctic Expedition in 2019 were used. The number of colony-forming units of microorganisms exhibiting proteolytic, amylolytic, cellulase, lipolytic activity was studied. Pure bacterial cultures were isolated using standard microbiological methods. Determination of resistance of isolates to heavy metals was estimated after their cultivation during ten days on agar plates with different concentrations of CdCl2 ⋅ 2.5H2O, CoCl2 ⋅ 6H2O, K2Cr2O7, FeSO4 ⋅ 7H2O, CuCl2 ⋅ 2H2O. Identification of strains was based on the sequencing of the 16S rRNA gene, morphological, physiological, and biochemical properties. Among the 23 isolates, nine metal-resistant strains were selected, four of which were identified as Pseudomonas yamanorum ІМV B-7916 and 79_102, and as P. arsenicoxidans 5A_1N_24, and 89_1T_89. Among the selected strains, the most resistant to heavy metals was P. yamanorum 79_102. All studied strains synthesize lipases during growth on medium with tween-20, which contains 0.5–1 mM of ferrous sulfate and copper (II) chloride. The studied strains produce exopolysaccharides during growth at 6 and 22 °C. The most effective among these strains exopolysaccharides are synthesized by P. arsenicoxidans 5A_1N_24 — 768 mg/g of dry weight. Our results expand the knowledge about the diversity of microorganisms of extreme biotopes, their properties, resistance to heavy metal compounds. Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic Ukrainian Antarctic Journal 2 58 75 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Ukrainian |
topic |
enzymatic activity exopolysaccharides metal resistance phylogenetic reconstruction pseudomonas arsenicoxidans pseudomonas yamanorum Meteorology. Climatology QC851-999 Geophysics. Cosmic physics QC801-809 |
spellingShingle |
enzymatic activity exopolysaccharides metal resistance phylogenetic reconstruction pseudomonas arsenicoxidans pseudomonas yamanorum Meteorology. Climatology QC851-999 Geophysics. Cosmic physics QC801-809 S. Hnatush S. Komplikevych O. Maslovska O. Moroz T. Peretyatko А. Dzhulai T. Krasnozhon Bacteria of the genus Pseudomonas isolated from Antarctic substrates |
topic_facet |
enzymatic activity exopolysaccharides metal resistance phylogenetic reconstruction pseudomonas arsenicoxidans pseudomonas yamanorum Meteorology. Climatology QC851-999 Geophysics. Cosmic physics QC801-809 |
description |
The study’s primary purposes were establishing the number of microorganisms that exhibit hydrolytic activity in Antarctic soil and mosses samples, isolation of metal-resistant strains of bacteria, and description of their physiological and biochemical properties. Samples collected during the XXIII Ukrainian Antarctic Expedition in 2019 were used. The number of colony-forming units of microorganisms exhibiting proteolytic, amylolytic, cellulase, lipolytic activity was studied. Pure bacterial cultures were isolated using standard microbiological methods. Determination of resistance of isolates to heavy metals was estimated after their cultivation during ten days on agar plates with different concentrations of CdCl2 ⋅ 2.5H2O, CoCl2 ⋅ 6H2O, K2Cr2O7, FeSO4 ⋅ 7H2O, CuCl2 ⋅ 2H2O. Identification of strains was based on the sequencing of the 16S rRNA gene, morphological, physiological, and biochemical properties. Among the 23 isolates, nine metal-resistant strains were selected, four of which were identified as Pseudomonas yamanorum ІМV B-7916 and 79_102, and as P. arsenicoxidans 5A_1N_24, and 89_1T_89. Among the selected strains, the most resistant to heavy metals was P. yamanorum 79_102. All studied strains synthesize lipases during growth on medium with tween-20, which contains 0.5–1 mM of ferrous sulfate and copper (II) chloride. The studied strains produce exopolysaccharides during growth at 6 and 22 °C. The most effective among these strains exopolysaccharides are synthesized by P. arsenicoxidans 5A_1N_24 — 768 mg/g of dry weight. Our results expand the knowledge about the diversity of microorganisms of extreme biotopes, their properties, resistance to heavy metal compounds. |
format |
Article in Journal/Newspaper |
author |
S. Hnatush S. Komplikevych O. Maslovska O. Moroz T. Peretyatko А. Dzhulai T. Krasnozhon |
author_facet |
S. Hnatush S. Komplikevych O. Maslovska O. Moroz T. Peretyatko А. Dzhulai T. Krasnozhon |
author_sort |
S. Hnatush |
title |
Bacteria of the genus Pseudomonas isolated from Antarctic substrates |
title_short |
Bacteria of the genus Pseudomonas isolated from Antarctic substrates |
title_full |
Bacteria of the genus Pseudomonas isolated from Antarctic substrates |
title_fullStr |
Bacteria of the genus Pseudomonas isolated from Antarctic substrates |
title_full_unstemmed |
Bacteria of the genus Pseudomonas isolated from Antarctic substrates |
title_sort |
bacteria of the genus pseudomonas isolated from antarctic substrates |
publisher |
State Institution National Antarctic Scientific Center |
publishDate |
2021 |
url |
https://doi.org/10.33275/1727-7485.2.2021.678 https://doaj.org/article/da94d2c8d1114195844aaa4bc9c0079c |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Український антарктичний журнал, Iss 2, Pp 58-75 (2021) |
op_relation |
http://uaj.uac.gov.ua/index.php/uaj/article/view/678 https://doaj.org/toc/1727-7485 https://doaj.org/toc/2415-3087 1727-7485 2415-3087 doi:10.33275/1727-7485.2.2021.678 https://doaj.org/article/da94d2c8d1114195844aaa4bc9c0079c |
op_doi |
https://doi.org/10.33275/1727-7485.2.2021.678 |
container_title |
Ukrainian Antarctic Journal |
container_issue |
2 |
container_start_page |
58 |
op_container_end_page |
75 |
_version_ |
1766263559308705792 |