Cytokine Profiles and Cell Proliferation Responses to Truncated ORF2 Protein in Iranian Patients Recovered from Hepatitis E Infection
Background. The aim of this study was to evaluate hepatitis E virus (HEV) specific cellular immune responses to truncated ORF2 protein in Iranian patients recovered from HEV infection. Information about HEV-specific immune responses could be useful in finding an effective way for development of HEV...
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ftdoajarticles:oai:doaj.org/article:da7bf915b76548158b5c9ebd084dfff6 2024-09-09T19:26:01+00:00 Cytokine Profiles and Cell Proliferation Responses to Truncated ORF2 Protein in Iranian Patients Recovered from Hepatitis E Infection Reza Taherkhani Fatemeh Farshadpour Manoochehr Makvandi Hamid Rajabi Memari Ali Reza Samarbafzadeh Nasrin Sharifi Behrouz Naeimi Saeed Tajbakhsh Samad Akbarzadeh 2015-01-01T00:00:00Z https://doi.org/10.1155/2015/523560 https://doaj.org/article/da7bf915b76548158b5c9ebd084dfff6 EN eng Wiley http://dx.doi.org/10.1155/2015/523560 https://doaj.org/toc/1687-9686 https://doaj.org/toc/1687-9694 1687-9686 1687-9694 doi:10.1155/2015/523560 https://doaj.org/article/da7bf915b76548158b5c9ebd084dfff6 Journal of Tropical Medicine, Vol 2015 (2015) Arctic medicine. Tropical medicine RC955-962 article 2015 ftdoajarticles https://doi.org/10.1155/2015/523560 2024-08-05T17:48:44Z Background. The aim of this study was to evaluate hepatitis E virus (HEV) specific cellular immune responses to truncated ORF2 protein in Iranian patients recovered from HEV infection. Information about HEV-specific immune responses could be useful in finding an effective way for development of HEV vaccine. Methods. A truncated form of HEV ORF2 protein containing amino acids 112-608 was used to stimulate peripheral blood mononuclear cells (PBMCs) separated from HEV-recovered and control groups. Finally, the levels of four cytokines, IFN-γ ELISPOT, and cell proliferative responses following stimulation with the truncated ORF2 protein were assessed in the both groups. Results. The truncated ORF2 protein was able to induce IFN-γ ELISPOT and cell proliferation responses and to produce significant amounts of IFN-γ and IL-12 cytokines, but low amounts of IL-10 and IL-4 cytokines in vitro. These responses were significantly higher in the recovered group compared to the control group. These results indicate the antigenic nature of the truncated ORF2 protein and production of T helper type 1 cytokines. Conclusion. The truncated ORF2 protein can effectively induce significant cellular immune responsesand can be introduced as a potential vaccine candidate. However, further studies are required to evaluate this protein in vivo. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Tropical Medicine 2015 1 7 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Reza Taherkhani Fatemeh Farshadpour Manoochehr Makvandi Hamid Rajabi Memari Ali Reza Samarbafzadeh Nasrin Sharifi Behrouz Naeimi Saeed Tajbakhsh Samad Akbarzadeh Cytokine Profiles and Cell Proliferation Responses to Truncated ORF2 Protein in Iranian Patients Recovered from Hepatitis E Infection |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 |
description |
Background. The aim of this study was to evaluate hepatitis E virus (HEV) specific cellular immune responses to truncated ORF2 protein in Iranian patients recovered from HEV infection. Information about HEV-specific immune responses could be useful in finding an effective way for development of HEV vaccine. Methods. A truncated form of HEV ORF2 protein containing amino acids 112-608 was used to stimulate peripheral blood mononuclear cells (PBMCs) separated from HEV-recovered and control groups. Finally, the levels of four cytokines, IFN-γ ELISPOT, and cell proliferative responses following stimulation with the truncated ORF2 protein were assessed in the both groups. Results. The truncated ORF2 protein was able to induce IFN-γ ELISPOT and cell proliferation responses and to produce significant amounts of IFN-γ and IL-12 cytokines, but low amounts of IL-10 and IL-4 cytokines in vitro. These responses were significantly higher in the recovered group compared to the control group. These results indicate the antigenic nature of the truncated ORF2 protein and production of T helper type 1 cytokines. Conclusion. The truncated ORF2 protein can effectively induce significant cellular immune responsesand can be introduced as a potential vaccine candidate. However, further studies are required to evaluate this protein in vivo. |
format |
Article in Journal/Newspaper |
author |
Reza Taherkhani Fatemeh Farshadpour Manoochehr Makvandi Hamid Rajabi Memari Ali Reza Samarbafzadeh Nasrin Sharifi Behrouz Naeimi Saeed Tajbakhsh Samad Akbarzadeh |
author_facet |
Reza Taherkhani Fatemeh Farshadpour Manoochehr Makvandi Hamid Rajabi Memari Ali Reza Samarbafzadeh Nasrin Sharifi Behrouz Naeimi Saeed Tajbakhsh Samad Akbarzadeh |
author_sort |
Reza Taherkhani |
title |
Cytokine Profiles and Cell Proliferation Responses to Truncated ORF2 Protein in Iranian Patients Recovered from Hepatitis E Infection |
title_short |
Cytokine Profiles and Cell Proliferation Responses to Truncated ORF2 Protein in Iranian Patients Recovered from Hepatitis E Infection |
title_full |
Cytokine Profiles and Cell Proliferation Responses to Truncated ORF2 Protein in Iranian Patients Recovered from Hepatitis E Infection |
title_fullStr |
Cytokine Profiles and Cell Proliferation Responses to Truncated ORF2 Protein in Iranian Patients Recovered from Hepatitis E Infection |
title_full_unstemmed |
Cytokine Profiles and Cell Proliferation Responses to Truncated ORF2 Protein in Iranian Patients Recovered from Hepatitis E Infection |
title_sort |
cytokine profiles and cell proliferation responses to truncated orf2 protein in iranian patients recovered from hepatitis e infection |
publisher |
Wiley |
publishDate |
2015 |
url |
https://doi.org/10.1155/2015/523560 https://doaj.org/article/da7bf915b76548158b5c9ebd084dfff6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Tropical Medicine, Vol 2015 (2015) |
op_relation |
http://dx.doi.org/10.1155/2015/523560 https://doaj.org/toc/1687-9686 https://doaj.org/toc/1687-9694 1687-9686 1687-9694 doi:10.1155/2015/523560 https://doaj.org/article/da7bf915b76548158b5c9ebd084dfff6 |
op_doi |
https://doi.org/10.1155/2015/523560 |
container_title |
Journal of Tropical Medicine |
container_volume |
2015 |
container_start_page |
1 |
op_container_end_page |
7 |
_version_ |
1809895723524685824 |