Extreme Viral Partitioning in a Marine-Derived High Arctic Lake
ABSTRACT High-latitude, perennially stratified (meromictic) lakes are likely to be especially vulnerable to climate warming because of the importance of ice in maintaining their water column structure and associated distribution of microbial communities. This study aimed to characterize viral abunda...
Published in: | mSphere |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
American Society for Microbiology
2020
|
Subjects: | |
Online Access: | https://doi.org/10.1128/mSphere.00334-20 https://doaj.org/article/da3ec7af94de4ecca4aae615141f4162 |
id |
ftdoajarticles:oai:doaj.org/article:da3ec7af94de4ecca4aae615141f4162 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:da3ec7af94de4ecca4aae615141f4162 2023-05-15T13:51:42+02:00 Extreme Viral Partitioning in a Marine-Derived High Arctic Lake Myriam Labbé Catherine Girard Warwick F. Vincent Alexander I. Culley 2020-06-01T00:00:00Z https://doi.org/10.1128/mSphere.00334-20 https://doaj.org/article/da3ec7af94de4ecca4aae615141f4162 EN eng American Society for Microbiology https://journals.asm.org/doi/10.1128/mSphere.00334-20 https://doaj.org/toc/2379-5042 doi:10.1128/mSphere.00334-20 2379-5042 https://doaj.org/article/da3ec7af94de4ecca4aae615141f4162 mSphere, Vol 5, Iss 3 (2020) aquatic viral ecology limnology polar science viromics Microbiology QR1-502 article 2020 ftdoajarticles https://doi.org/10.1128/mSphere.00334-20 2022-12-31T11:28:27Z ABSTRACT High-latitude, perennially stratified (meromictic) lakes are likely to be especially vulnerable to climate warming because of the importance of ice in maintaining their water column structure and associated distribution of microbial communities. This study aimed to characterize viral abundance, diversity, and distribution in a meromictic lake of marine origin on the far northern coast of Ellesmere Island, in the Canadian High Arctic. We collected triplicate samples for double-stranded DNA (dsDNA) viromics from five depths that encompassed the major features of the lake, as determined by limnological profiling of the water column. Viral abundance and virus-to-prokaryote ratios were highest at greater depths, while bacterial and cyanobacterial counts were greatest in the surface waters. The viral communities from each zone of the lake defined by salinity, temperature, and dissolved oxygen concentrations were markedly distinct, suggesting that there was little exchange of viral types among lake strata. Ten viral assembled genomes were obtained from our libraries, and these also segregated with depth. This well-defined structure of viral communities was consistent with that of potential hosts. Viruses from the monimolimnion, a deep layer of ancient Arctic Ocean seawater, were more diverse and relatively abundant, with few similarities to available viral sequences. The Lake A viral communities also differed from published records from the Arctic Ocean and meromictic Ace Lake in Antarctica. This first characterization of viral diversity from this sentinel environment underscores the microbial richness and complexity of an ecosystem type that is increasingly exposed to major perturbations in the fast-changing Arctic. IMPORTANCE The Arctic is warming at an accelerating pace, and the rise in temperature has increasing impacts on the Arctic biome. Lakes are integrators of their surroundings and thus excellent sentinels of environmental change. Despite their importance in the regulation of key microbial processes, ... Article in Journal/Newspaper Antarc* Antarctica Arctic Arctic Ocean Ellesmere Island Directory of Open Access Journals: DOAJ Articles Ace Lake ENVELOPE(78.188,78.188,-68.472,-68.472) Arctic Arctic Lake ENVELOPE(-130.826,-130.826,57.231,57.231) Arctic Ocean Ellesmere Island mSphere 5 3 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
aquatic viral ecology limnology polar science viromics Microbiology QR1-502 |
spellingShingle |
aquatic viral ecology limnology polar science viromics Microbiology QR1-502 Myriam Labbé Catherine Girard Warwick F. Vincent Alexander I. Culley Extreme Viral Partitioning in a Marine-Derived High Arctic Lake |
topic_facet |
aquatic viral ecology limnology polar science viromics Microbiology QR1-502 |
description |
ABSTRACT High-latitude, perennially stratified (meromictic) lakes are likely to be especially vulnerable to climate warming because of the importance of ice in maintaining their water column structure and associated distribution of microbial communities. This study aimed to characterize viral abundance, diversity, and distribution in a meromictic lake of marine origin on the far northern coast of Ellesmere Island, in the Canadian High Arctic. We collected triplicate samples for double-stranded DNA (dsDNA) viromics from five depths that encompassed the major features of the lake, as determined by limnological profiling of the water column. Viral abundance and virus-to-prokaryote ratios were highest at greater depths, while bacterial and cyanobacterial counts were greatest in the surface waters. The viral communities from each zone of the lake defined by salinity, temperature, and dissolved oxygen concentrations were markedly distinct, suggesting that there was little exchange of viral types among lake strata. Ten viral assembled genomes were obtained from our libraries, and these also segregated with depth. This well-defined structure of viral communities was consistent with that of potential hosts. Viruses from the monimolimnion, a deep layer of ancient Arctic Ocean seawater, were more diverse and relatively abundant, with few similarities to available viral sequences. The Lake A viral communities also differed from published records from the Arctic Ocean and meromictic Ace Lake in Antarctica. This first characterization of viral diversity from this sentinel environment underscores the microbial richness and complexity of an ecosystem type that is increasingly exposed to major perturbations in the fast-changing Arctic. IMPORTANCE The Arctic is warming at an accelerating pace, and the rise in temperature has increasing impacts on the Arctic biome. Lakes are integrators of their surroundings and thus excellent sentinels of environmental change. Despite their importance in the regulation of key microbial processes, ... |
format |
Article in Journal/Newspaper |
author |
Myriam Labbé Catherine Girard Warwick F. Vincent Alexander I. Culley |
author_facet |
Myriam Labbé Catherine Girard Warwick F. Vincent Alexander I. Culley |
author_sort |
Myriam Labbé |
title |
Extreme Viral Partitioning in a Marine-Derived High Arctic Lake |
title_short |
Extreme Viral Partitioning in a Marine-Derived High Arctic Lake |
title_full |
Extreme Viral Partitioning in a Marine-Derived High Arctic Lake |
title_fullStr |
Extreme Viral Partitioning in a Marine-Derived High Arctic Lake |
title_full_unstemmed |
Extreme Viral Partitioning in a Marine-Derived High Arctic Lake |
title_sort |
extreme viral partitioning in a marine-derived high arctic lake |
publisher |
American Society for Microbiology |
publishDate |
2020 |
url |
https://doi.org/10.1128/mSphere.00334-20 https://doaj.org/article/da3ec7af94de4ecca4aae615141f4162 |
long_lat |
ENVELOPE(78.188,78.188,-68.472,-68.472) ENVELOPE(-130.826,-130.826,57.231,57.231) |
geographic |
Ace Lake Arctic Arctic Lake Arctic Ocean Ellesmere Island |
geographic_facet |
Ace Lake Arctic Arctic Lake Arctic Ocean Ellesmere Island |
genre |
Antarc* Antarctica Arctic Arctic Ocean Ellesmere Island |
genre_facet |
Antarc* Antarctica Arctic Arctic Ocean Ellesmere Island |
op_source |
mSphere, Vol 5, Iss 3 (2020) |
op_relation |
https://journals.asm.org/doi/10.1128/mSphere.00334-20 https://doaj.org/toc/2379-5042 doi:10.1128/mSphere.00334-20 2379-5042 https://doaj.org/article/da3ec7af94de4ecca4aae615141f4162 |
op_doi |
https://doi.org/10.1128/mSphere.00334-20 |
container_title |
mSphere |
container_volume |
5 |
container_issue |
3 |
_version_ |
1766255725635436544 |