Genomic analysis of natural intra-specific hybrids among Ethiopian isolates of Leishmania donovani.
Parasites of the genus Leishmania (Kinetoplastida: Trypanosomatidae) cause widespread and devastating human diseases. Visceral leishmaniasis due to Leishmania donovani is endemic in Ethiopia where it has also been responsible for major epidemics. The presence of hybrid genotypes has been widely repo...
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ftdoajarticles:oai:doaj.org/article:d9f15aeeab6442db9ad35fa34fc31606 2023-05-15T15:10:02+02:00 Genomic analysis of natural intra-specific hybrids among Ethiopian isolates of Leishmania donovani. James A Cotton Caroline Durrant Susanne U Franssen Tesfaye Gelanew Asrat Hailu David Mateus Mandy J Sanders Matthew Berriman Petr Volf Michael A Miles Matthew Yeo 2020-04-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007143 https://doaj.org/article/d9f15aeeab6442db9ad35fa34fc31606 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007143 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007143 https://doaj.org/article/d9f15aeeab6442db9ad35fa34fc31606 PLoS Neglected Tropical Diseases, Vol 14, Iss 4, p e0007143 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007143 2022-12-31T07:16:57Z Parasites of the genus Leishmania (Kinetoplastida: Trypanosomatidae) cause widespread and devastating human diseases. Visceral leishmaniasis due to Leishmania donovani is endemic in Ethiopia where it has also been responsible for major epidemics. The presence of hybrid genotypes has been widely reported in surveys of natural populations, genetic variation reported in a number of Leishmania species, and the extant capacity for genetic exchange demonstrated in laboratory experiments. However, patterns of recombination and the evolutionary history of admixture that produced these hybrid populations remain unclear. Here, we use whole-genome sequence data to investigate Ethiopian L. donovani isolates previously characterized as hybrids by microsatellite and multi-locus sequencing. To date there is only one previous study on a natural population of Leishmania hybrids based on whole-genome sequences. We propose that these hybrids originate from recombination between two different lineages of Ethiopian L. donovani occurring in the same region. Patterns of inheritance are more complex than previously reported with multiple, apparently independent, origins from similar parents that include backcrossing with parental types. Analysis indicates that hybrids are representative of at least three different histories. Furthermore, isolates were highly polysomic at the level of chromosomes with differences between parasites recovered from a recrudescent infection from a previously treated individual. The results demonstrate that recombination is a significant feature of natural populations and contributes to the growing body of data that shows how recombination, and gene flow, shape natural populations of Leishmania. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 4 e0007143 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 James A Cotton Caroline Durrant Susanne U Franssen Tesfaye Gelanew Asrat Hailu David Mateus Mandy J Sanders Matthew Berriman Petr Volf Michael A Miles Matthew Yeo Genomic analysis of natural intra-specific hybrids among Ethiopian isolates of Leishmania donovani. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Parasites of the genus Leishmania (Kinetoplastida: Trypanosomatidae) cause widespread and devastating human diseases. Visceral leishmaniasis due to Leishmania donovani is endemic in Ethiopia where it has also been responsible for major epidemics. The presence of hybrid genotypes has been widely reported in surveys of natural populations, genetic variation reported in a number of Leishmania species, and the extant capacity for genetic exchange demonstrated in laboratory experiments. However, patterns of recombination and the evolutionary history of admixture that produced these hybrid populations remain unclear. Here, we use whole-genome sequence data to investigate Ethiopian L. donovani isolates previously characterized as hybrids by microsatellite and multi-locus sequencing. To date there is only one previous study on a natural population of Leishmania hybrids based on whole-genome sequences. We propose that these hybrids originate from recombination between two different lineages of Ethiopian L. donovani occurring in the same region. Patterns of inheritance are more complex than previously reported with multiple, apparently independent, origins from similar parents that include backcrossing with parental types. Analysis indicates that hybrids are representative of at least three different histories. Furthermore, isolates were highly polysomic at the level of chromosomes with differences between parasites recovered from a recrudescent infection from a previously treated individual. The results demonstrate that recombination is a significant feature of natural populations and contributes to the growing body of data that shows how recombination, and gene flow, shape natural populations of Leishmania. |
format |
Article in Journal/Newspaper |
author |
James A Cotton Caroline Durrant Susanne U Franssen Tesfaye Gelanew Asrat Hailu David Mateus Mandy J Sanders Matthew Berriman Petr Volf Michael A Miles Matthew Yeo |
author_facet |
James A Cotton Caroline Durrant Susanne U Franssen Tesfaye Gelanew Asrat Hailu David Mateus Mandy J Sanders Matthew Berriman Petr Volf Michael A Miles Matthew Yeo |
author_sort |
James A Cotton |
title |
Genomic analysis of natural intra-specific hybrids among Ethiopian isolates of Leishmania donovani. |
title_short |
Genomic analysis of natural intra-specific hybrids among Ethiopian isolates of Leishmania donovani. |
title_full |
Genomic analysis of natural intra-specific hybrids among Ethiopian isolates of Leishmania donovani. |
title_fullStr |
Genomic analysis of natural intra-specific hybrids among Ethiopian isolates of Leishmania donovani. |
title_full_unstemmed |
Genomic analysis of natural intra-specific hybrids among Ethiopian isolates of Leishmania donovani. |
title_sort |
genomic analysis of natural intra-specific hybrids among ethiopian isolates of leishmania donovani. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0007143 https://doaj.org/article/d9f15aeeab6442db9ad35fa34fc31606 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 4, p e0007143 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007143 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007143 https://doaj.org/article/d9f15aeeab6442db9ad35fa34fc31606 |
op_doi |
https://doi.org/10.1371/journal.pntd.0007143 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
14 |
container_issue |
4 |
container_start_page |
e0007143 |
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1766341110161997824 |