Host Response of Atlantic Salmon ( Salmo salar ) Re-Inoculated with Paramoeba perurans
In aquaculture, recurrence rates of amoebic gill disease (AGD) caused by the ectoparasite Paramoeba perurans are high and no prophylactic strategies exist for disease prevention. In this study, Atlantic salmon ( Salmo salar ) were initially inoculated with P. perurans and following the development o...
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2021
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ftdoajarticles:oai:doaj.org/article:d9cdeee86b9e4b92ac6df194b40874cd 2023-05-15T15:31:19+02:00 Host Response of Atlantic Salmon ( Salmo salar ) Re-Inoculated with Paramoeba perurans Michelle McCormack Anita Talbot Eugene Dillon Ian O’Connor Eugene MacCarthy 2021-05-01T00:00:00Z https://doi.org/10.3390/microorganisms9050993 https://doaj.org/article/d9cdeee86b9e4b92ac6df194b40874cd EN eng MDPI AG https://www.mdpi.com/2076-2607/9/5/993 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms9050993 2076-2607 https://doaj.org/article/d9cdeee86b9e4b92ac6df194b40874cd Microorganisms, Vol 9, Iss 993, p 993 (2021) amoebic gill disease immune response gill proteome repeat inoculation Biology (General) QH301-705.5 article 2021 ftdoajarticles https://doi.org/10.3390/microorganisms9050993 2022-12-31T13:51:00Z In aquaculture, recurrence rates of amoebic gill disease (AGD) caused by the ectoparasite Paramoeba perurans are high and no prophylactic strategies exist for disease prevention. In this study, Atlantic salmon ( Salmo salar ) were initially inoculated with P. perurans and following the development of amoebic gill disease were treated with freshwater immersion on day 21 and day 35 post inoculation. Fish were re-inoculated following a negative qPCR analysis for the presence of P. perurans . The gill host immune response was investigated at 7, 14, and 18 days post re-inoculation. Differential proteome expression of immune related proteins was assessed by comparison of each time point against naïve controls. In the gill, some proteins of the innate immune system were expressed in response to gill re-colonization by P. perurans , while no features of adaptive immunity were found to be differentially expressed. Many of the proteins identified are novel in the context of AGD and their expression profiles suggest that their roles in the response to disease development and progression in single or multiple infections warrant further investigation. Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles Microorganisms 9 5 993 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
amoebic gill disease immune response gill proteome repeat inoculation Biology (General) QH301-705.5 |
spellingShingle |
amoebic gill disease immune response gill proteome repeat inoculation Biology (General) QH301-705.5 Michelle McCormack Anita Talbot Eugene Dillon Ian O’Connor Eugene MacCarthy Host Response of Atlantic Salmon ( Salmo salar ) Re-Inoculated with Paramoeba perurans |
topic_facet |
amoebic gill disease immune response gill proteome repeat inoculation Biology (General) QH301-705.5 |
description |
In aquaculture, recurrence rates of amoebic gill disease (AGD) caused by the ectoparasite Paramoeba perurans are high and no prophylactic strategies exist for disease prevention. In this study, Atlantic salmon ( Salmo salar ) were initially inoculated with P. perurans and following the development of amoebic gill disease were treated with freshwater immersion on day 21 and day 35 post inoculation. Fish were re-inoculated following a negative qPCR analysis for the presence of P. perurans . The gill host immune response was investigated at 7, 14, and 18 days post re-inoculation. Differential proteome expression of immune related proteins was assessed by comparison of each time point against naïve controls. In the gill, some proteins of the innate immune system were expressed in response to gill re-colonization by P. perurans , while no features of adaptive immunity were found to be differentially expressed. Many of the proteins identified are novel in the context of AGD and their expression profiles suggest that their roles in the response to disease development and progression in single or multiple infections warrant further investigation. |
format |
Article in Journal/Newspaper |
author |
Michelle McCormack Anita Talbot Eugene Dillon Ian O’Connor Eugene MacCarthy |
author_facet |
Michelle McCormack Anita Talbot Eugene Dillon Ian O’Connor Eugene MacCarthy |
author_sort |
Michelle McCormack |
title |
Host Response of Atlantic Salmon ( Salmo salar ) Re-Inoculated with Paramoeba perurans |
title_short |
Host Response of Atlantic Salmon ( Salmo salar ) Re-Inoculated with Paramoeba perurans |
title_full |
Host Response of Atlantic Salmon ( Salmo salar ) Re-Inoculated with Paramoeba perurans |
title_fullStr |
Host Response of Atlantic Salmon ( Salmo salar ) Re-Inoculated with Paramoeba perurans |
title_full_unstemmed |
Host Response of Atlantic Salmon ( Salmo salar ) Re-Inoculated with Paramoeba perurans |
title_sort |
host response of atlantic salmon ( salmo salar ) re-inoculated with paramoeba perurans |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/microorganisms9050993 https://doaj.org/article/d9cdeee86b9e4b92ac6df194b40874cd |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Microorganisms, Vol 9, Iss 993, p 993 (2021) |
op_relation |
https://www.mdpi.com/2076-2607/9/5/993 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms9050993 2076-2607 https://doaj.org/article/d9cdeee86b9e4b92ac6df194b40874cd |
op_doi |
https://doi.org/10.3390/microorganisms9050993 |
container_title |
Microorganisms |
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9 |
container_issue |
5 |
container_start_page |
993 |
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1766361811347570688 |