Differential Regulation of the Expression of the Two Thyrotropin Beta Subunit Paralogs by Salmon Pituitary Cells In Vitro
We recently characterized two paralogs of the thyrotropin (TSH) beta subunit in Atlantic salmon, tshβa and tshβb, issued from teleost-specific whole genome duplication. The transcript expression of tshβb, but not of tshβa, peaks at the time of smoltification, which revealed a specific involvement of...
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ftdoajarticles:oai:doaj.org/article:d9b5471aa314425484bb5cc7de8dff3c 2023-05-15T15:32:09+02:00 Differential Regulation of the Expression of the Two Thyrotropin Beta Subunit Paralogs by Salmon Pituitary Cells In Vitro Mitchell Stewart Fleming Gersende Maugars Patrick Martin Sylvie Dufour Karine Rousseau 2020-11-01T00:00:00Z https://doi.org/10.3389/fendo.2020.603538 https://doaj.org/article/d9b5471aa314425484bb5cc7de8dff3c EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fendo.2020.603538/full https://doaj.org/toc/1664-2392 1664-2392 doi:10.3389/fendo.2020.603538 https://doaj.org/article/d9b5471aa314425484bb5cc7de8dff3c Frontiers in Endocrinology, Vol 11 (2020) tshβ paralogs gh thyroid hormones cortisol IGF1 CRH Diseases of the endocrine glands. Clinical endocrinology RC648-665 article 2020 ftdoajarticles https://doi.org/10.3389/fendo.2020.603538 2022-12-31T09:51:57Z We recently characterized two paralogs of the thyrotropin (TSH) beta subunit in Atlantic salmon, tshβa and tshβb, issued from teleost-specific whole genome duplication. The transcript expression of tshβb, but not of tshβa, peaks at the time of smoltification, which revealed a specific involvement of tshβb paralog in this metamorphic event. Tshβa and tshβb are expressed by distinct pituitary cells in salmon, likely related to TSH cells from the pars distalis and pars tuberalis, respectively, in mammals and birds. The present study aimed at investigating the neuroendocrine and endocrine factors potentially involved in the differential regulation of tshβa and tshβb paralogs, using primary cultures of Atlantic salmon pituitary cells. The effects of various neurohormones and endocrine factors potentially involved in the control of development, growth, and metabolism were tested. Transcript levels of tshβa and tshβb were measured by qPCR, as well as those of growth hormone (gh), for comparison and validation. Corticotropin-releasing hormone (CRH) stimulated tshβa transcript levels in agreement with its potential role in the thyrotropic axis in teleosts, but had no effect on tshβb paralog, while it also stimulated gh transcript levels. Thyrotropin-releasing hormone (TRH) had no effect on neither tshβ paralogs nor gh. Somatostatin (SRIH) had no effects on both tshβ paralogs, while it exerted a canonical inhibitory effect on gh transcript levels. Thyroid hormones [triiodothyronine (T3) and thyroxine (T4)] inhibited transcript levels of both tshβ paralogs, as well as gh, but with a much stronger effect on tshβa than on tshβb and gh. Conversely, cortisol had a stronger inhibitory effect on tshβb than tshβa, while no effect on gh. Remarkably, insulin-like growth factor 1 (IGF1) dose-dependently stimulated tshβb transcript levels, while it had no effect on tshβa, and a classical inhibitory effect on gh. This study provides the first data on the neuroendocrine factors involved in the differential regulation of the expression ... Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles Frontiers in Endocrinology 11 |
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English |
topic |
tshβ paralogs gh thyroid hormones cortisol IGF1 CRH Diseases of the endocrine glands. Clinical endocrinology RC648-665 |
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tshβ paralogs gh thyroid hormones cortisol IGF1 CRH Diseases of the endocrine glands. Clinical endocrinology RC648-665 Mitchell Stewart Fleming Gersende Maugars Patrick Martin Sylvie Dufour Karine Rousseau Differential Regulation of the Expression of the Two Thyrotropin Beta Subunit Paralogs by Salmon Pituitary Cells In Vitro |
topic_facet |
tshβ paralogs gh thyroid hormones cortisol IGF1 CRH Diseases of the endocrine glands. Clinical endocrinology RC648-665 |
description |
We recently characterized two paralogs of the thyrotropin (TSH) beta subunit in Atlantic salmon, tshβa and tshβb, issued from teleost-specific whole genome duplication. The transcript expression of tshβb, but not of tshβa, peaks at the time of smoltification, which revealed a specific involvement of tshβb paralog in this metamorphic event. Tshβa and tshβb are expressed by distinct pituitary cells in salmon, likely related to TSH cells from the pars distalis and pars tuberalis, respectively, in mammals and birds. The present study aimed at investigating the neuroendocrine and endocrine factors potentially involved in the differential regulation of tshβa and tshβb paralogs, using primary cultures of Atlantic salmon pituitary cells. The effects of various neurohormones and endocrine factors potentially involved in the control of development, growth, and metabolism were tested. Transcript levels of tshβa and tshβb were measured by qPCR, as well as those of growth hormone (gh), for comparison and validation. Corticotropin-releasing hormone (CRH) stimulated tshβa transcript levels in agreement with its potential role in the thyrotropic axis in teleosts, but had no effect on tshβb paralog, while it also stimulated gh transcript levels. Thyrotropin-releasing hormone (TRH) had no effect on neither tshβ paralogs nor gh. Somatostatin (SRIH) had no effects on both tshβ paralogs, while it exerted a canonical inhibitory effect on gh transcript levels. Thyroid hormones [triiodothyronine (T3) and thyroxine (T4)] inhibited transcript levels of both tshβ paralogs, as well as gh, but with a much stronger effect on tshβa than on tshβb and gh. Conversely, cortisol had a stronger inhibitory effect on tshβb than tshβa, while no effect on gh. Remarkably, insulin-like growth factor 1 (IGF1) dose-dependently stimulated tshβb transcript levels, while it had no effect on tshβa, and a classical inhibitory effect on gh. This study provides the first data on the neuroendocrine factors involved in the differential regulation of the expression ... |
format |
Article in Journal/Newspaper |
author |
Mitchell Stewart Fleming Gersende Maugars Patrick Martin Sylvie Dufour Karine Rousseau |
author_facet |
Mitchell Stewart Fleming Gersende Maugars Patrick Martin Sylvie Dufour Karine Rousseau |
author_sort |
Mitchell Stewart Fleming |
title |
Differential Regulation of the Expression of the Two Thyrotropin Beta Subunit Paralogs by Salmon Pituitary Cells In Vitro |
title_short |
Differential Regulation of the Expression of the Two Thyrotropin Beta Subunit Paralogs by Salmon Pituitary Cells In Vitro |
title_full |
Differential Regulation of the Expression of the Two Thyrotropin Beta Subunit Paralogs by Salmon Pituitary Cells In Vitro |
title_fullStr |
Differential Regulation of the Expression of the Two Thyrotropin Beta Subunit Paralogs by Salmon Pituitary Cells In Vitro |
title_full_unstemmed |
Differential Regulation of the Expression of the Two Thyrotropin Beta Subunit Paralogs by Salmon Pituitary Cells In Vitro |
title_sort |
differential regulation of the expression of the two thyrotropin beta subunit paralogs by salmon pituitary cells in vitro |
publisher |
Frontiers Media S.A. |
publishDate |
2020 |
url |
https://doi.org/10.3389/fendo.2020.603538 https://doaj.org/article/d9b5471aa314425484bb5cc7de8dff3c |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Frontiers in Endocrinology, Vol 11 (2020) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fendo.2020.603538/full https://doaj.org/toc/1664-2392 1664-2392 doi:10.3389/fendo.2020.603538 https://doaj.org/article/d9b5471aa314425484bb5cc7de8dff3c |
op_doi |
https://doi.org/10.3389/fendo.2020.603538 |
container_title |
Frontiers in Endocrinology |
container_volume |
11 |
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1766362662583664640 |