Immunophenotypic characterization of patients with American cutaneous leishmaniasis prior to and after treatment in Pernambuco, Brazil
Leishmania infections induce a specific activation of host immunological response, particularly characterized by T cell expansion. Studies indicate the importance of the balance between CD4+ and CD8+ T cells, in which the first ones would have their number reduced during the healing process. Meanwhi...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:d9b3e148c05c4d7785b655b2dfab3613 2023-05-15T15:08:05+02:00 Immunophenotypic characterization of patients with American cutaneous leishmaniasis prior to and after treatment in Pernambuco, Brazil AF Almeida MCAB Castro AP Oliveira MA Souza VRA Pereira 2011-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992011000200016 https://doaj.org/article/d9b3e148c05c4d7785b655b2dfab3613 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200016 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992011000200016 1678-9199 https://doaj.org/article/d9b3e148c05c4d7785b655b2dfab3613 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 17, Iss 2, Pp 230-234 (2011) American tegumentary leishmaniasis cellular immune response flow cytometry Leishmania (Viannia) braziliensis Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2011 ftdoajarticles https://doi.org/10.1590/S1678-91992011000200016 2022-12-31T01:59:55Z Leishmania infections induce a specific activation of host immunological response, particularly characterized by T cell expansion. Studies indicate the importance of the balance between CD4+ and CD8+ T cells, in which the first ones would have their number reduced during the healing process. Meanwhile, CD25+ T cells have been associated with the suppression of the immune response. Since the immune response has an essential role in both healing and progression of diseases, this study aimed to identify the percentage of CD3+, CD4+, CD8+, CD16+ and CD25+ T cells in the peripheral blood of patients afflicted with American cutaneous leishmaniasis (ACL) - before and after treatment - and healthy controls. Peripheral blood was collected and transferred to cytometry tubes containing monoclonal antibodies specific for cell surface markers CD3, CD4, CD8, CD16 e CD25. The immunophenotypic and morphometric parameters of cells were determined by flow cytometry and the results demonstrated a significant increase in the number of T CD8+ cells after treatment, suggesting a cytotoxic T cell response. An increase in CD25+ T cells in patients with active ACL and after treatment was also observed, suggesting the participation of these cells in the modulation of the exacerbated effector response. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 17 2 230 234 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
American tegumentary leishmaniasis cellular immune response flow cytometry Leishmania (Viannia) braziliensis Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
American tegumentary leishmaniasis cellular immune response flow cytometry Leishmania (Viannia) braziliensis Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 AF Almeida MCAB Castro AP Oliveira MA Souza VRA Pereira Immunophenotypic characterization of patients with American cutaneous leishmaniasis prior to and after treatment in Pernambuco, Brazil |
topic_facet |
American tegumentary leishmaniasis cellular immune response flow cytometry Leishmania (Viannia) braziliensis Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Leishmania infections induce a specific activation of host immunological response, particularly characterized by T cell expansion. Studies indicate the importance of the balance between CD4+ and CD8+ T cells, in which the first ones would have their number reduced during the healing process. Meanwhile, CD25+ T cells have been associated with the suppression of the immune response. Since the immune response has an essential role in both healing and progression of diseases, this study aimed to identify the percentage of CD3+, CD4+, CD8+, CD16+ and CD25+ T cells in the peripheral blood of patients afflicted with American cutaneous leishmaniasis (ACL) - before and after treatment - and healthy controls. Peripheral blood was collected and transferred to cytometry tubes containing monoclonal antibodies specific for cell surface markers CD3, CD4, CD8, CD16 e CD25. The immunophenotypic and morphometric parameters of cells were determined by flow cytometry and the results demonstrated a significant increase in the number of T CD8+ cells after treatment, suggesting a cytotoxic T cell response. An increase in CD25+ T cells in patients with active ACL and after treatment was also observed, suggesting the participation of these cells in the modulation of the exacerbated effector response. |
format |
Article in Journal/Newspaper |
author |
AF Almeida MCAB Castro AP Oliveira MA Souza VRA Pereira |
author_facet |
AF Almeida MCAB Castro AP Oliveira MA Souza VRA Pereira |
author_sort |
AF Almeida |
title |
Immunophenotypic characterization of patients with American cutaneous leishmaniasis prior to and after treatment in Pernambuco, Brazil |
title_short |
Immunophenotypic characterization of patients with American cutaneous leishmaniasis prior to and after treatment in Pernambuco, Brazil |
title_full |
Immunophenotypic characterization of patients with American cutaneous leishmaniasis prior to and after treatment in Pernambuco, Brazil |
title_fullStr |
Immunophenotypic characterization of patients with American cutaneous leishmaniasis prior to and after treatment in Pernambuco, Brazil |
title_full_unstemmed |
Immunophenotypic characterization of patients with American cutaneous leishmaniasis prior to and after treatment in Pernambuco, Brazil |
title_sort |
immunophenotypic characterization of patients with american cutaneous leishmaniasis prior to and after treatment in pernambuco, brazil |
publisher |
SciELO |
publishDate |
2011 |
url |
https://doi.org/10.1590/S1678-91992011000200016 https://doaj.org/article/d9b3e148c05c4d7785b655b2dfab3613 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 17, Iss 2, Pp 230-234 (2011) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200016 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992011000200016 1678-9199 https://doaj.org/article/d9b3e148c05c4d7785b655b2dfab3613 |
op_doi |
https://doi.org/10.1590/S1678-91992011000200016 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
17 |
container_issue |
2 |
container_start_page |
230 |
op_container_end_page |
234 |
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1766339512202428416 |