Isolation and characterization of fast‐growing green snow bacteria from coastal East Antarctica
Abstract Snow microorganisms play a significant role in climate change and affecting the snow melting rate in the Arctic and Antarctic regions. While research on algae inhabiting green and red snow has been performed extensively, bacteria dwelling in this biotope have been studied to a much lesser e...
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ftdoajarticles:oai:doaj.org/article:d98f3370788d40699e346b74e7a7fc7f 2023-05-15T13:59:28+02:00 Isolation and characterization of fast‐growing green snow bacteria from coastal East Antarctica Margarita Smirnova Uladzislau Miamin Achim Kohler Leonid Valentovich Artur Akhremchuk Anastasiya Sidarenka Andrey Dolgikh Volha Shapaval 2021-02-01T00:00:00Z https://doi.org/10.1002/mbo3.1152 https://doaj.org/article/d98f3370788d40699e346b74e7a7fc7f EN eng Wiley https://doi.org/10.1002/mbo3.1152 https://doaj.org/toc/2045-8827 2045-8827 doi:10.1002/mbo3.1152 https://doaj.org/article/d98f3370788d40699e346b74e7a7fc7f MicrobiologyOpen, Vol 10, Iss 1, Pp n/a-n/a (2021) 16S rRNA gene amplicon sequencing 16S rRNA gene sequencing Antarctic bacteria Fourier transform infrared (FTIR) spectroscopy green snow principal component analysis Microbiology QR1-502 article 2021 ftdoajarticles https://doi.org/10.1002/mbo3.1152 2022-12-31T06:32:09Z Abstract Snow microorganisms play a significant role in climate change and affecting the snow melting rate in the Arctic and Antarctic regions. While research on algae inhabiting green and red snow has been performed extensively, bacteria dwelling in this biotope have been studied to a much lesser extent. In this study, we performed 16S rRNA gene amplicon sequencing of two green snow samples collected from the coastal area of the eastern part of Antarctica and conducted genotypic and phenotypic profiling of 45 fast‐growing bacteria isolated from these samples. 16S rRNA gene amplicon sequencing of two green snow samples showed that bacteria inhabiting these samples are mostly represented by families Burkholderiaceae (46.31%), Flavobacteriaceae (22.98%), and Pseudomonadaceae (17.66%). Identification of 45 fast‐growing bacteria isolated from green snow was performed using 16S rRNA gene sequencing. We demonstrated that they belong to the phyla Actinobacteria and Proteobacteria, and are represented by the genera Arthrobacter, Cryobacterium, Leifsonia, Salinibacterium, Paeniglutamicibacter, Rhodococcus, Polaromonas, Pseudomonas, and Psychrobacter. Nearly all bacterial isolates exhibited various growth temperatures from 4°C to 25°C, and some isolates were characterized by a high level of enzymatic activity. Phenotyping using Fourier transform infrared (FTIR) spectroscopy revealed a possible accumulation of intracellular polymer polyhydroxyalkanoates (PHA) or lipids in some isolates. The bacteria showed different lipids/PHA and protein profiles. It was shown that lipid/PHA and protein spectral regions are the most discriminative for differentiating the isolates. Article in Journal/Newspaper Antarc* Antarctic Antarctica Arctic Climate change East Antarctica Directory of Open Access Journals: DOAJ Articles Antarctic Arctic East Antarctica MicrobiologyOpen 10 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
16S rRNA gene amplicon sequencing 16S rRNA gene sequencing Antarctic bacteria Fourier transform infrared (FTIR) spectroscopy green snow principal component analysis Microbiology QR1-502 |
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16S rRNA gene amplicon sequencing 16S rRNA gene sequencing Antarctic bacteria Fourier transform infrared (FTIR) spectroscopy green snow principal component analysis Microbiology QR1-502 Margarita Smirnova Uladzislau Miamin Achim Kohler Leonid Valentovich Artur Akhremchuk Anastasiya Sidarenka Andrey Dolgikh Volha Shapaval Isolation and characterization of fast‐growing green snow bacteria from coastal East Antarctica |
topic_facet |
16S rRNA gene amplicon sequencing 16S rRNA gene sequencing Antarctic bacteria Fourier transform infrared (FTIR) spectroscopy green snow principal component analysis Microbiology QR1-502 |
description |
Abstract Snow microorganisms play a significant role in climate change and affecting the snow melting rate in the Arctic and Antarctic regions. While research on algae inhabiting green and red snow has been performed extensively, bacteria dwelling in this biotope have been studied to a much lesser extent. In this study, we performed 16S rRNA gene amplicon sequencing of two green snow samples collected from the coastal area of the eastern part of Antarctica and conducted genotypic and phenotypic profiling of 45 fast‐growing bacteria isolated from these samples. 16S rRNA gene amplicon sequencing of two green snow samples showed that bacteria inhabiting these samples are mostly represented by families Burkholderiaceae (46.31%), Flavobacteriaceae (22.98%), and Pseudomonadaceae (17.66%). Identification of 45 fast‐growing bacteria isolated from green snow was performed using 16S rRNA gene sequencing. We demonstrated that they belong to the phyla Actinobacteria and Proteobacteria, and are represented by the genera Arthrobacter, Cryobacterium, Leifsonia, Salinibacterium, Paeniglutamicibacter, Rhodococcus, Polaromonas, Pseudomonas, and Psychrobacter. Nearly all bacterial isolates exhibited various growth temperatures from 4°C to 25°C, and some isolates were characterized by a high level of enzymatic activity. Phenotyping using Fourier transform infrared (FTIR) spectroscopy revealed a possible accumulation of intracellular polymer polyhydroxyalkanoates (PHA) or lipids in some isolates. The bacteria showed different lipids/PHA and protein profiles. It was shown that lipid/PHA and protein spectral regions are the most discriminative for differentiating the isolates. |
format |
Article in Journal/Newspaper |
author |
Margarita Smirnova Uladzislau Miamin Achim Kohler Leonid Valentovich Artur Akhremchuk Anastasiya Sidarenka Andrey Dolgikh Volha Shapaval |
author_facet |
Margarita Smirnova Uladzislau Miamin Achim Kohler Leonid Valentovich Artur Akhremchuk Anastasiya Sidarenka Andrey Dolgikh Volha Shapaval |
author_sort |
Margarita Smirnova |
title |
Isolation and characterization of fast‐growing green snow bacteria from coastal East Antarctica |
title_short |
Isolation and characterization of fast‐growing green snow bacteria from coastal East Antarctica |
title_full |
Isolation and characterization of fast‐growing green snow bacteria from coastal East Antarctica |
title_fullStr |
Isolation and characterization of fast‐growing green snow bacteria from coastal East Antarctica |
title_full_unstemmed |
Isolation and characterization of fast‐growing green snow bacteria from coastal East Antarctica |
title_sort |
isolation and characterization of fast‐growing green snow bacteria from coastal east antarctica |
publisher |
Wiley |
publishDate |
2021 |
url |
https://doi.org/10.1002/mbo3.1152 https://doaj.org/article/d98f3370788d40699e346b74e7a7fc7f |
geographic |
Antarctic Arctic East Antarctica |
geographic_facet |
Antarctic Arctic East Antarctica |
genre |
Antarc* Antarctic Antarctica Arctic Climate change East Antarctica |
genre_facet |
Antarc* Antarctic Antarctica Arctic Climate change East Antarctica |
op_source |
MicrobiologyOpen, Vol 10, Iss 1, Pp n/a-n/a (2021) |
op_relation |
https://doi.org/10.1002/mbo3.1152 https://doaj.org/toc/2045-8827 2045-8827 doi:10.1002/mbo3.1152 https://doaj.org/article/d98f3370788d40699e346b74e7a7fc7f |
op_doi |
https://doi.org/10.1002/mbo3.1152 |
container_title |
MicrobiologyOpen |
container_volume |
10 |
container_issue |
1 |
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1766268036581425152 |