Chagas' disease in the Brazilian Amazon: II. A serological survey Doença de Chagas na Amazônia brasileira: II. Inquérito sorológico
A serological survey, involving indirect immunofluorescence testing of blood sera samples, was carried out on the residents of one in every five dwellings in the town of Barcelos (in the northern part of the State of Amazonas, on the right bank of the Rio Negro, 490 Km from Manaus by river) and on t...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
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Language: | English |
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Universidade de São Paulo (USP)
1995
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Online Access: | https://doi.org/10.1590/S0036-46651995000200003 https://doaj.org/article/d9724aefae3e4040922f2bf72fe6d556 |
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ftdoajarticles:oai:doaj.org/article:d9724aefae3e4040922f2bf72fe6d556 2024-09-09T19:28:40+00:00 Chagas' disease in the Brazilian Amazon: II. A serological survey Doença de Chagas na Amazônia brasileira: II. Inquérito sorológico José Rodrigues Coura Margarita Arboleda Naranjo Henry Percy Faraco Willcox 1995-04-01T00:00:00Z https://doi.org/10.1590/S0036-46651995000200003 https://doaj.org/article/d9724aefae3e4040922f2bf72fe6d556 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651995000200003 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651995000200003 0036-4665 1678-9946 https://doaj.org/article/d9724aefae3e4040922f2bf72fe6d556 Revista do Instituto de Medicina Tropical de São Paulo, Vol 37, Iss 2, Pp 103-107 (1995) Chagas' disease Serological survey Brazilian Amazon Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 1995 ftdoajarticles https://doi.org/10.1590/S0036-46651995000200003 2024-08-05T17:49:30Z A serological survey, involving indirect immunofluorescence testing of blood sera samples, was carried out on the residents of one in every five dwellings in the town of Barcelos (in the northern part of the State of Amazonas, on the right bank of the Rio Negro, 490 Km from Manaus by river) and on the rural populations of the villages of Piloto and Marará (also on the right bank of the Rio Negro, 30 minutes by boat from Barcelos). A total of 710 sera samples were tested, 628 from the resident population in the town of Barcelos, 35 from Piloto and 47 from Marará. The tests were carried out using human anti-gammaglobulin type IgG (Biolab) and antigen from formolized culture of T.cruzi Y strain. The sera were serially diluted from 1:40 to 1:320 in PBS 7.2. Of the 710 samples examined 89(12.5%) were positive for anti-T.cruzi antibodies: 2 of these (2.2%) at a dilution of 1:320; 12(13.4%) at 1:160; 38 (42.6%) at 1:80; and the remainder at 1:40, giving a median serological dilution of 1:80. The following questions are discussed: the high serological prevalence for Chagas'infection found in our survey; the possibility of serological cross-reactions; the need for confirmatory tests for the positives reactions; and the strong correlation between our results and preliminary epidemiological data (such as the level of human contact with wild triatominae, know locally as "Piacava's lice". We draw attention to the isolation by xenodiagnosis of one strain of T.cruzi from a patient with positive serology for Chagas' infection. Foi realizado um inquérito sorológico através da reação de imunofluorescência indireta em uma amostra de soro sanguíneo da população residente em uma em cada cinco casas habitadas na sede do município de Barcelos, localizado no norte do Estado do Amazonas, na margem direita do Rio Negro, a 490 km de Manaus por via fluvial e na população das localidades rurais de Piloto e Marará, localizadas também na margem direita do Rio Negro, a 30 minutos por via fluvial da sede do município de Barcelos. No total foram ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 37 2 103 107 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Chagas' disease Serological survey Brazilian Amazon Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Chagas' disease Serological survey Brazilian Amazon Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 José Rodrigues Coura Margarita Arboleda Naranjo Henry Percy Faraco Willcox Chagas' disease in the Brazilian Amazon: II. A serological survey Doença de Chagas na Amazônia brasileira: II. Inquérito sorológico |
topic_facet |
Chagas' disease Serological survey Brazilian Amazon Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
A serological survey, involving indirect immunofluorescence testing of blood sera samples, was carried out on the residents of one in every five dwellings in the town of Barcelos (in the northern part of the State of Amazonas, on the right bank of the Rio Negro, 490 Km from Manaus by river) and on the rural populations of the villages of Piloto and Marará (also on the right bank of the Rio Negro, 30 minutes by boat from Barcelos). A total of 710 sera samples were tested, 628 from the resident population in the town of Barcelos, 35 from Piloto and 47 from Marará. The tests were carried out using human anti-gammaglobulin type IgG (Biolab) and antigen from formolized culture of T.cruzi Y strain. The sera were serially diluted from 1:40 to 1:320 in PBS 7.2. Of the 710 samples examined 89(12.5%) were positive for anti-T.cruzi antibodies: 2 of these (2.2%) at a dilution of 1:320; 12(13.4%) at 1:160; 38 (42.6%) at 1:80; and the remainder at 1:40, giving a median serological dilution of 1:80. The following questions are discussed: the high serological prevalence for Chagas'infection found in our survey; the possibility of serological cross-reactions; the need for confirmatory tests for the positives reactions; and the strong correlation between our results and preliminary epidemiological data (such as the level of human contact with wild triatominae, know locally as "Piacava's lice". We draw attention to the isolation by xenodiagnosis of one strain of T.cruzi from a patient with positive serology for Chagas' infection. Foi realizado um inquérito sorológico através da reação de imunofluorescência indireta em uma amostra de soro sanguíneo da população residente em uma em cada cinco casas habitadas na sede do município de Barcelos, localizado no norte do Estado do Amazonas, na margem direita do Rio Negro, a 490 km de Manaus por via fluvial e na população das localidades rurais de Piloto e Marará, localizadas também na margem direita do Rio Negro, a 30 minutos por via fluvial da sede do município de Barcelos. No total foram ... |
format |
Article in Journal/Newspaper |
author |
José Rodrigues Coura Margarita Arboleda Naranjo Henry Percy Faraco Willcox |
author_facet |
José Rodrigues Coura Margarita Arboleda Naranjo Henry Percy Faraco Willcox |
author_sort |
José Rodrigues Coura |
title |
Chagas' disease in the Brazilian Amazon: II. A serological survey Doença de Chagas na Amazônia brasileira: II. Inquérito sorológico |
title_short |
Chagas' disease in the Brazilian Amazon: II. A serological survey Doença de Chagas na Amazônia brasileira: II. Inquérito sorológico |
title_full |
Chagas' disease in the Brazilian Amazon: II. A serological survey Doença de Chagas na Amazônia brasileira: II. Inquérito sorológico |
title_fullStr |
Chagas' disease in the Brazilian Amazon: II. A serological survey Doença de Chagas na Amazônia brasileira: II. Inquérito sorológico |
title_full_unstemmed |
Chagas' disease in the Brazilian Amazon: II. A serological survey Doença de Chagas na Amazônia brasileira: II. Inquérito sorológico |
title_sort |
chagas' disease in the brazilian amazon: ii. a serological survey doença de chagas na amazônia brasileira: ii. inquérito sorológico |
publisher |
Universidade de São Paulo (USP) |
publishDate |
1995 |
url |
https://doi.org/10.1590/S0036-46651995000200003 https://doaj.org/article/d9724aefae3e4040922f2bf72fe6d556 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 37, Iss 2, Pp 103-107 (1995) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651995000200003 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651995000200003 0036-4665 1678-9946 https://doaj.org/article/d9724aefae3e4040922f2bf72fe6d556 |
op_doi |
https://doi.org/10.1590/S0036-46651995000200003 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
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37 |
container_issue |
2 |
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103 |
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107 |
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