High-throughput analysis of synthetic peptides for the immunodiagnosis of canine visceral leishmaniasis.
BACKGROUND: Visceral leishmaniasis is the most severe form of leishmaniasis. Approximately 20% of zoonotic human visceral leishmaniasis worldwide is caused by Leishmania infantum, which is also known as Leishmania chagasi in Latin America, and disease incidence is increasing in urban and peri-urban...
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ftdoajarticles:oai:doaj.org/article:d94142dd8df2458b99f2c8c51584bfbd 2023-05-15T15:15:56+02:00 High-throughput analysis of synthetic peptides for the immunodiagnosis of canine visceral leishmaniasis. Angélica R Faria Míriam M Costa Mário S Giusta Gabriel Grimaldi Marcus L O Penido Ricardo T Gazzinelli Hélida M Andrade 2011-09-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001310 https://doaj.org/article/d94142dd8df2458b99f2c8c51584bfbd EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3172188?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001310 https://doaj.org/article/d94142dd8df2458b99f2c8c51584bfbd PLoS Neglected Tropical Diseases, Vol 5, Iss 9, p e1310 (2011) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2011 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001310 2022-12-31T15:02:20Z BACKGROUND: Visceral leishmaniasis is the most severe form of leishmaniasis. Approximately 20% of zoonotic human visceral leishmaniasis worldwide is caused by Leishmania infantum, which is also known as Leishmania chagasi in Latin America, and disease incidence is increasing in urban and peri-urban areas of the tropics. In this form of disease, dogs are the main reservoirs. Diagnostic methods used to identify Leishmania infected animals are not able to detect all of the infected ones, which can compromise the effectiveness of disease control. Therefore, to contribute to the improvement of diagnostic methods for canine visceral leishmaniasis (CVL), we aimed to identify and test novel antigens using high-throughput analysis. METHODOLOGY/PRINCIPAL FINDINGS: Immunodominant proteins from L. infantum were mapped in silico to predict B cell epitopes, and the 360 predicted peptides were synthesized on cellulose membranes. Immunoassays were used to select the most reactive peptides, which were then investigated with canine sera. Next, the 10 most reactive peptides were synthesized using solid phase peptide synthesis protocol and tested using ELISA. The sensitivity and specificity of these peptides were also compared to the EIE-LVC Bio-Manguinhos kit, which is recommended by the Brazilian Ministry of Health for use in leishmaniasis control programs. The sensitivity and specificity of the selected synthesized peptides was as high as 88.70% and 95.00%, respectively, whereas the EIE-LVC kit had a sensitivity of 13.08% and 100.00% of specificity. Although the tests based on synthetic peptides were able to diagnose up to 94.80% of asymptomatic dogs with leishmaniasis, the EIE-LVC kit failed to detect the disease in any of the infected asymptomatic dogs. CONCLUSIONS/SIGNIFICANCE: Our study shows that ELISA using synthetic peptides is a technique with great potential for diagnosing CVL; furthermore, the use of these peptides in other diagnostic methodologies, such as immunochromatographic tests, could be beneficial to CVL ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Eie ENVELOPE(7.983,7.983,63.033,63.033) PLoS Neglected Tropical Diseases 5 9 e1310 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Angélica R Faria Míriam M Costa Mário S Giusta Gabriel Grimaldi Marcus L O Penido Ricardo T Gazzinelli Hélida M Andrade High-throughput analysis of synthetic peptides for the immunodiagnosis of canine visceral leishmaniasis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: Visceral leishmaniasis is the most severe form of leishmaniasis. Approximately 20% of zoonotic human visceral leishmaniasis worldwide is caused by Leishmania infantum, which is also known as Leishmania chagasi in Latin America, and disease incidence is increasing in urban and peri-urban areas of the tropics. In this form of disease, dogs are the main reservoirs. Diagnostic methods used to identify Leishmania infected animals are not able to detect all of the infected ones, which can compromise the effectiveness of disease control. Therefore, to contribute to the improvement of diagnostic methods for canine visceral leishmaniasis (CVL), we aimed to identify and test novel antigens using high-throughput analysis. METHODOLOGY/PRINCIPAL FINDINGS: Immunodominant proteins from L. infantum were mapped in silico to predict B cell epitopes, and the 360 predicted peptides were synthesized on cellulose membranes. Immunoassays were used to select the most reactive peptides, which were then investigated with canine sera. Next, the 10 most reactive peptides were synthesized using solid phase peptide synthesis protocol and tested using ELISA. The sensitivity and specificity of these peptides were also compared to the EIE-LVC Bio-Manguinhos kit, which is recommended by the Brazilian Ministry of Health for use in leishmaniasis control programs. The sensitivity and specificity of the selected synthesized peptides was as high as 88.70% and 95.00%, respectively, whereas the EIE-LVC kit had a sensitivity of 13.08% and 100.00% of specificity. Although the tests based on synthetic peptides were able to diagnose up to 94.80% of asymptomatic dogs with leishmaniasis, the EIE-LVC kit failed to detect the disease in any of the infected asymptomatic dogs. CONCLUSIONS/SIGNIFICANCE: Our study shows that ELISA using synthetic peptides is a technique with great potential for diagnosing CVL; furthermore, the use of these peptides in other diagnostic methodologies, such as immunochromatographic tests, could be beneficial to CVL ... |
format |
Article in Journal/Newspaper |
author |
Angélica R Faria Míriam M Costa Mário S Giusta Gabriel Grimaldi Marcus L O Penido Ricardo T Gazzinelli Hélida M Andrade |
author_facet |
Angélica R Faria Míriam M Costa Mário S Giusta Gabriel Grimaldi Marcus L O Penido Ricardo T Gazzinelli Hélida M Andrade |
author_sort |
Angélica R Faria |
title |
High-throughput analysis of synthetic peptides for the immunodiagnosis of canine visceral leishmaniasis. |
title_short |
High-throughput analysis of synthetic peptides for the immunodiagnosis of canine visceral leishmaniasis. |
title_full |
High-throughput analysis of synthetic peptides for the immunodiagnosis of canine visceral leishmaniasis. |
title_fullStr |
High-throughput analysis of synthetic peptides for the immunodiagnosis of canine visceral leishmaniasis. |
title_full_unstemmed |
High-throughput analysis of synthetic peptides for the immunodiagnosis of canine visceral leishmaniasis. |
title_sort |
high-throughput analysis of synthetic peptides for the immunodiagnosis of canine visceral leishmaniasis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2011 |
url |
https://doi.org/10.1371/journal.pntd.0001310 https://doaj.org/article/d94142dd8df2458b99f2c8c51584bfbd |
long_lat |
ENVELOPE(7.983,7.983,63.033,63.033) |
geographic |
Arctic Eie |
geographic_facet |
Arctic Eie |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 5, Iss 9, p e1310 (2011) |
op_relation |
http://europepmc.org/articles/PMC3172188?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001310 https://doaj.org/article/d94142dd8df2458b99f2c8c51584bfbd |
op_doi |
https://doi.org/10.1371/journal.pntd.0001310 |
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PLoS Neglected Tropical Diseases |
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5 |
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9 |
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e1310 |
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1766346251841830912 |